95 results about "Albumin nanoparticles" patented technology

The present invention provides combination therapy methods of treating proliferative diseases (such as cancer) comprising a first therapy comprising administering to an individual an effective amount of a taxane in a nanoparticle composition, and a second therapy which may include, for example, radiation, surgery, administration of chemotherapeutic agents (such as an anti-VEGF antibody), or combinations thereof. Also provided are methods of administering to an individual a drug taxane in a nanoparticle composition based on a metronomic dosing regime.

A process for producing nanoparticles of paclitaxel and albumin having antitumor properties, by which a mixture obtained by adding paclitaxel in powder form to an aqueous solution of albumin with chloroform is subjected to high pressure homogenization treatment.

The invention relates to a method for preparing a stable albumin nanoparticle, and belongs to the technical field of preparation of biomedical materials. The method comprises the following steps: pretreating albumin by using glutathione and cysteine without biotoxicity; opening an intramolecular disulfide bond; precipitating the albumin by using anti-solvents such as alcohol and the like; and carrying out exchange reaction on a sulfydryl-disulfide bond to obtain the albumin nanoparticle containing the intramolecular disulfide bond. The prepared albumin nanoparticle can be used for the delivery of pharmacological active substances and / or diagnostic agents in an organism. The albumin nanoparticle provided by the invention has the advantages that the albumin nanoparticle has good stability under a dilution condition and gives an oxidation reduction response in a reduced environment. Based on the characteristics, the albumin nanoparticle can stably exist in a blood circulation system of the organism, and can carry out a degradation reaction in a cell under the action of reduced glutathione so as to release a wrapped medicine.

The present invention discloses Near Infrared (NIR) fluorescent albumin nanoparticles having a structure selected from a core structure or a core-shell structure. Also disclosed are a process of preparing these NIR fluorescent albumin nanoparticles, and a method of in vivo detection of pathologies, in particular cancer pathology, by using administering these NIR fluorescent albumin nanoparticles to a patient.

The invention provides a pharmaceutical composition of curcumin and albumin nano particles, and a preparation method of the pharmaceutical composition. The albumin is made from human serum albumin or bovine serum albumin, or is prepared by taking ovalbumin as a raw material; and preferentially, the albumin is made from human serum albumin. The pharmaceutical composition prepared by the invention has better encapsulation rate.

The invention belongs to the field of preparation of biological medical materials, and relates to a method for preparing stable albumin nano-particles by virtue of thermal denaturation. The method comprises the following steps: (1) adding vanillic aldehyde or an analogue thereof to form intermolecular disulfide bonds by virtue of inter-reaction of free sulfhydryl groups on albumin molecules under a heating condition; (2) enabling amino groups inside and among molecules to react with carboxyl so as to form amido bonds; and (3) enabling amino groups on the albumin molecules to react with aldehyde groups on vanillic aldehyde or the analogue thereof to form chemical bonds of Schiff base and the like so as to form stable nano-particles in an aqueous solution. Any organic solvent is not introduced during preparation, so that the prepared nano-particles are safe and nontoxic, and can well entrap antitumor drugs including paclitaxel, doxorubicin hydrochloride and the like. Moreover, the carrier has an oxidation reduction response in a tumor cell internal environment, and can open disulfide bonds to release drugs under the action of reducing glutathione in cells. The method provided by the invention is simple in process, convenient to operate and suitable for industrial mass production.

The invention provides a cabazitaxel albumin nanoparticle preparation and a preparation method thereof. The cabazitaxel albumin nanoparticle preparation comprises cabazitaxel, albumin and pharmaceutically necessary auxiliary materials and can be prepared with a high-pressure homogenization method, a film shearing method, a solvent evaporation method and the like. According to the cabazitaxel albumin nanoparticle preparation and the preparation method thereof, problems of the drug loading capacity and the stability of existing albumin nanoparticles are solved; a low-toxicity organic solvent is adopted, so that highly allergic reactions due to the fact that Tween-80 is adopted in a cabazitaxel injection liquid can be avoided; by the aid of the high permeability and the retention effect of the nanoparticle on tumors, more drugs can be targeted passively to be concentrated in tumor tissue, so that the anti-tumor effect is improved; the preparation method is simple and suitable for industrial mass production.

An arsenic compound solution, and an albumin nanoparticle and a lyophilized preparation prepared using same and entrapping an arsenic compound. The arsenic compound solution is prepared using the following method: adding As2O3 powder in sterile deionized water to obtain a suspension, 6 to 25 mg of As2O3 powder being added in every mL of sterile deionized water; dripping an NaOH solution to the obtained suspension until the powder is fully dissolved and adjusting the pH of the solution to 7.5 to 9, and making the concentration of the obtained arsenic compound solution be 5 to 20 mg / mL in terms of the added As2O3. The albumin nanoparticle is prepared by mixing an albumin solution and the arsenic solution and the arsenic solution with the mass ratio of the added As2O3 in arsenic compound solution to the albumin ranging from 1:5 to 1:20 and using a method of solvent removing and physical curing.

The present invention discloses albumin nanoparticles, a preparation method and applications, wherein the albumin nanoparticles and an antibody or antibody drug conjugate (such as doxorubicin) are subjected to adsorption connection. According to the present invention, the experiment results show that: compared with the single drug, the nanoparticles modified with the antibody or antibody drug conjugate have advantages of enhancement of targeting property, and reduction of toxic-side effects of drugs on the body; and the formed albumin nanoparticles use the enhanced permeability and retention effect (EPR) of the tumor tissue to enhance the intratumoral accumulation so as to provide a certain slow release effect and a certain drug efflux inhibition effect, enhance the biological utilization rate of the drug, and reduce the drug consumption, such that the efficient and low-toxicity nanometer treatment way is provided for the tumor targeting therapy.

The invention belongs to the technical field of biomedicines and discloses albumin nanoparticles covering pharmacological active substances as well as a preparation method and application thereof. Thepreparation method comprises the following steps: opening an inner space structure of albumin under the action of glutathione to form protein containing a sulfydryl group active radical; adding a selenium compound and the pharmacological active substances; obtaining the albumin nanoparticles covering the pharmacological active substances by utilizing intramolecular or intermolecular sulfydryl-selenium-sulfur bond exchange reaction and sulfydryl-disulfide bond exchange reaction and elemental selenium which is loaded at inner and outer parts of a protein cavity. The method has the advantages ofsimplicity in operation; a novel albumin binding type nano-preparation has the advantages of uniform size, good dispersity and long preservation time at room temperature, and good stability in gastric acid, intestinal juice and blood serum; the solubility, dispersity, stability and bioavailability of lipid-soluble medicines are greatly improved. Meanwhile, the albumin nano-preparation keeps the solubility of albumin and a tumor targeting enriching property very well.

The invention provides an albumin composition highly loaded with cabazitaxel and its preparation and preparation method. In the albumin composition highly loaded with cabazitaxel drug of the present invention, the carrier albumin in the form of albumin nanoparticles and non-nanoparticles encapsulates the drug loading of cabazitaxel up to 13% to 25% (weight), and The carrier albumin in the form of nanoparticles accounts for more than 50% by weight of the total albumin carriers. The composition of the present invention is convenient for clinical use, greatly reduces the amount of albumin as an auxiliary material, and more efficiently entraps drugs, delivers them to lesions, and plays a role.

The invention belongs to the field of medical technology and relates to an integrin targeting drug loaded albumin nanoparticle formulation and its preparation method. According to the invention, a gemcitabine-carried albumin nanoparticle target administration system is prepared by gemcitabine raw medicine, bovine serum albumin BSA, a RGD polypeptide, injection water, a dehydrating agent, a cross-linking agent and sodium hydroxide. The drug-loaded albumin nanoparticles have good dispersibility with a distribution range of particle size being 94-166nm, average particle size being 130nm, Zeta potential being -30.77mV, an encapsulation efficiency being 92.16%, a drug loading capacity being 12.8%, 30min burst release rate being 53.25+ / -2.23% and 8 hours in vitro cumulative release rate reaching 90%. It is verified through experiments that the targeted nanoparticles provided by the invention can significantly increase the drug accumulation in the target sites, have good slow release and tumor targeting effects, can improve therapeutic effects and relieve toxic and side effects of chemotherapeutic drugs and enhance the tolerance of patients to treatment.

A doxorubicin and TRAIL co-supported albumin nanoparticle targeting preparation and a preparation method thereof. The preparation consists of doxorubicin hydrochloride DOX.HCl, human tumor necrosis factor apoptosis ligand TRAIL, bovine serum albumin BSA, polyethyleneimine PEI, carboxymethyl chitosan-folate conjugates CMCS-FA, water for injection and a crosslinking agent, and is a doxorubicin and TRAIL entrapped albumin nanoparticle targeting drug delivery system. The method comprises preparation of doxorubicin supported albumin nanoparticles, synthesis of a carboxymethyl chitosan-folate conjugate (CMCS-FA), and preparation of doxorubicin and TRAIL co-entrapped folate targeting albumin nanoparticles. Folic acid mediated active targeting and tumor pH sensitive release enhance the accumulation of drug carrier in tumors; the supported doxorubicin and TRAIL can produce synergistic anticancer effect and have a strong killing effect on cancer cells (including drug-resistant cells).

The invention discloses albumin nanoparticles realizing co-delivery of an antitumor drug and an MRI (magnetic resonance imaging) contrast medium and a preparation method of the albumin nanoparticles and further provides an albumin nanoparticle vector containing the MRI contrast medium, and drug loading is realized through electrostatic adsorption and coordination between the drug and the vector as well as crosslinking of amino acid residues of the vector. The invention further discloses an optimal preparation technology of the albumin nanoparticles realizing co-delivery of the antitumor drug and the MRI contrast medium and a function of the albumin nanoparticles in diagnosis and treatment combination of tumors. According to the albumin nanoparticles realizing co-delivery of the antitumor drug and the MRI contrast medium, the drug uptake ratio of cells can be increased, and drug efflux caused by drug-resistant cells is reduced, so that drug resistance is reversed, and efficient delivery of the drug is realized; the albumin nanoparticles realizing co-delivery of the antitumor drug and the MRI contrast medium have excellent T1 weighted imaging capability and an effective means is provided for the diagnosis and treatment combination of the tumors.

The invention discloses perfluorocarbon albumin nanoparticles which comprise albumins and perfluorocarbon compounds, wherein the albumins coat the perfluorocarbon compounds, and the mass ratio of theperfluorocarbon compounds to the albumins is (4-40): 1. The perfluorocarbon albumin nanoparticles have good biological safety as a novel blood platelet inhibitor. The perfluorocarbon albumin nanoparticles which are administrated will induce great reduction of quantity of blood platelets in a human body, various functions of the blood platelets can be also inhibited obviously, and the perfluorocarbon albumin nanoparticles have relatively good clinical applications and practical treatment meaning.

The invention provides paclitaxel / resveratrol albumin nanoparticles and a preparation technology. The preparation technology comprises the following steps: (1) preparing a paclitaxel and resveratrol storage solution; (2) preparing an albumin storage solution; (3) preparing double-drug albumin nanoparticles. According to the paclitaxel / resveratrol albumin nanoparticles, an anti-cancer drug paclitaxel and a bioactive substance resveratrol are combined to be administrated, so that the toxic side effect can be reduced and the anti-cancer effect is improved; the prepared double-drug albumin nanoparticles have the encapsulation efficiency of 99 percent and have good stability; the double-drug albumin nanoparticles provided by the invention can be released in sequence; the resveratrol is rapidlyreleased so that the effect of inhibiting P-gp is facilitated; a condition that the paclitaxel which is released subsequently is pumped out from tumor cells is avoided, so that the cytotoxicity on thedrug-resisting tumor cells is increased; an in-vitro cytotoxicity experiment of the double-drug albumin nanoparticles provided by the invention proves that the drug resistance of the tumor cells canbe reversed, and a cooperative anti-tumor effect of the paclitaxel and the resveratrol in the double-drug nanoparticles is realized.

The invention discloses an ultrasound lipid microbubble wrapping drug-carrying albumin nanoparticles and a preparation method thereof. The drug-carrying albumin nanoparticles comprise drugs and albumin. An ultrasound lipid microbubble membrane material mainly comprises phospholipid components and can wrap gas and the drug-carrying albumin nanoparticles simultaneously to form a composite structure with the gas and the nanoparticles in the middle. According to the ultrasound lipid microbubble wrapping the drug-carrying albumin nanoparticles and the preparation method thereof, the drug-carrying capacity of the microbubble is high, the preparation technology is simple, the production cost is low, and the ultrasound lipid microbubble is prone to industrial production, not only can be taken as an ultrasound developer for disease diagnosis, but also can be taken as a drug-carrying preparation for disease treatment.

The invention belongs to the technical field of biomedical engineering nano medicaments and discloses a preparation method of a novel albumin nano medicament under a mild condition of 37 DEG C. The preparation method comprises the following steps of: 1) preparing an albumin aqueous solution; 2) preparing a reducing agent solution, adding the reducing agent solution into the albumin aqueous solution, adding a protein denaturing agent solution, and reacting for 0.5-6 hours at the temperature of between 30 and 80 DEG C to obtain a reduced albumin solution; 3) dialyzing or ultra-filtering the reduced albumin solution, and removing unreacted reducing agent and protein denaturing agent to obtain a reduced albumin nano solution; 4) mixing the reduced albumin nano solution with an acidic buffer solution to enable the pH value to be between 2.8 and 5.0, placing the mixture in an environment of 37 DEG C, stirring to obtain albumin nano particles, dialyzing, and freeze-drying to obtain the finalalbumin nano medicament. The albumin nanoparticles have very good serum stability and storage stability, stably exists in a body circulation system, increases the half-life of the medicament, and havereduction sensitivity characteristics.

The invention discloses a preparation method of hepatic targeting nanoparticles carried with curcumin based on albumin serving as carrier material, belongs to the field of medicine, and in particular relates to prepare the hepatic targeting nanoparticles, curcumin albumin nanoparticles modified by glycyrrhetinic acid, through covalent binding. Each nanoparticle carried with medicine comprises the curcumin, the albumin and the glycyrrhetinic acid. The hepatic targeting nanoparticles are formed by the glycyrrhetinic acid binding to the surfaces of the albumin nanoparticles carried with the medicine. The prepared targeting nanoparticles have the effect of releasing, the glycyrrhetinic acid can mediate the curcumin albumin nanoparticles to target to a hepatic cell, the toxic and side effect of the medicine are lowered, the curative effect of the medicine is improved, the preparation craft is simple, the production cost is low, and wide application prospect is possessed.

The invention relates to the field of pharmaceutical preparations, in particular to glaucocalyxin A-carrying bilirubin albumin nanoparticles, a preparation method thereof and application of the nanoparticles in breast cancer treatment.The prepared glaucocalyxin A-carrying bilirubin albumin nanoparticles can overcome the defects that glaucocalyxin A is poor in water solubility and is quickly removed in a body and difficult to administrate through the characters of slow releasing and targeting of albumin nanoparticles.The glaucocalyxin A-carrying bilirubin albumin nanoparticles have very good application prospects in aspects of medicine solubilizing, slow releasing, targeting and the like.

The invention provides breviscapine albumin nanoparticles prepared by adsorbing breviscapine through albumin nanoparticles. The breviscapine albumin nanoparticles comprise the breviscapine serving as an active ingredient and the albumin nanoparticles, and are obtained by adsorbing the prepared and freeze-dried albumin nanoparticles to the breviscapine under a certain condition to carry medicines. Through the breviscapine albumin nanoparticles prepared by adsorbing the breviscapine through the albumin nanoparticles, the problem of low dissolubility of breviscapine aqueous solution is solved; and the breviscapine albumin nanoparticles have obvious slow release characteristic.

The invention discloses a method for preparing medicine albumin nanoparticles, and belongs to the technical field of medicine preparations. According to the method disclosed by the invention, metal salt without biologic toxicity or a non-salt compound is used as a coagulator, a natural compound without biologic toxicity is used as a cross-linking agent, and a few of solvents which are nearly nontoxic, such as ethanol, are used as a medicine solvent. Nanoparticles are formed through energy instantaneously released through cavitation. The preparation method of the medicine albumin nanoparticles disclosed by the invention has the advantages that the preparation method is environmentally-friendly, organic solvents having medium and high toxins are not used, an emulsifying and volatilizing process is not included, besides, the solidification effect and the dispersion effects of the nanoparticles are completed at the same time, a few technology steps are used, and the time consumption is short. The particle diameter of the medicine albumin nanoparticles prepared by the method is generally smaller than 800nm.

The invention discloses a preparation method of novel bovine serum albumin covered thiacloprid pesticide nanoparticles. An emulsifying-curing method is adopted in a process of covering thiacloprid with bovine serum albumin; the preparation method comprises the following steps: firstly, dissolving a certain amount of the bovine serum albumin into double distilled water to prepare a water phase; dissolving the thiacloprid into acetonitrile to obtain an oil phase; stirring at a high speed and dropwise adding the oil phase into the water phase; emulsifying for a period of time and rapidly cooling; carrying out ultrasonic crushing to obtain super-micro emulsion; freezing and coldly drying to obtain thiacloprid albumin nanoparticles. According to a novel albumin nanometer preparation technology adopted by the method, pesticide is loaded into albumin under the condition that the structure of the albumin is not changed and the pesticide loading amount is increased; meanwhile, a product has a slow releasing function. A nanotechnology is adopted so that a pesticide product is miniaturized, the resource waste is reduced, the cost is reduced and the pesticide effect is remarkably improved; the contact area between the pesticide and target insects is effectively enlarged and the pesticide is more easily swallowed and absorbed; contact toxicity and stomach poisoning actions are expressed better; high efficiency, low toxicity and safety of nano biological pesticide are realized.

The present invention provides a metal-ICG complex, a preparation method of the complex, metal-ICG complex albumin nanoparticles and a preparation method and application of the nanoparticles, and relates to the technical field of nano-drugs. Metal ions of the metal-ICG complex are selected from at least one of gold, platinum, zinc, copper, cobalt, iron, nickel and manganese. The technical problemsof few types, short maximum illumination wavelength and narrow therapeutic window of an existing acoustic sensitive agent are improved; and the metal ions and ICG in the metal-ICG complex provided bythe invention are complexed by coordination bonds, so that the type of acoustic sensitive agents is expanded, the illumination wavelength of the acoustic sensitive agents is increased, the therapeutic window is extended, and the metal-ICG complex has double functions of photosensitivity and acoustic sensitivity and can effectively promote the development of photothermal and sonodynamic therapy.

The invention provides medicine-phospholipid / albumin composite nanoparticles and a preparation technology. The preparation technology comprises the following steps: (1) preparing paclitaxel, phospholipid, cholesterol and a DSPE-PEG2000 (Distearoyl Phosphoethanolamine-Polyethylene Glycol 2000) stock solution; (2) preparing a paclitaxel liposome; (3) preparing albumin (BSA) stock solution; and (4) preparing the paclitaxel-phospholipid / albumin composite nanoparticles. The preparation method provided by the invention is simple and the problem that the solubility of a difficult-to-dissolve medicinein water is poor can be solved; and a preparation does not contain Cremophor EL and a toxic or side effect caused by a solubilizer in an injection process is avoided. Being different from common liposomes and albumin nanoparticles, the medicine-phospholipid / albumin composite nanoparticles prepared by the preparation technology have the characteristics that DSPE-PEG2000 is added into a prescription so that the stability of the nanoparticles is increased; and the encapsulation efficiency is high, the grain diameter is small and uniform, and the grain diameter of the nanoparticles is not obviously changed after the nanoparticles are stored at room temperature for two weeks.

The invention belongs to the field of pharmaceutical preparation and relates to an albumin nanometer particle preparation for soluble injection. The albumin nanometer particle, which is formed by combining albumin, indissolvable erlotinib drugs and adding phospholipid for scattering and stabilizing, is prepared into the albumin nanometer particle preparation for soluble injection. The enhancing permeation and detaining effect of nanometer particle to tumor are utilized by the albumin nanometer particle preparation provided by the invention, so that more drugs are passively targeted and gathered on a tumor tissue and the anti-tumor effect is increased. Meanwhile, the bioavailability of injection mode is high, the albumin nanometer particle preparation for soluble injection has a passive targeting function and the dosage is greatly reduced, so that the concentration of the drugs in non-target part is efficiently reduced, the reducing of the toxic side effect of the drugs is boosted and the clinical application prospect is excellent.

The invention discloses ultrasonic activateable medicine-release albumin nanoparticles as well as a preparation method and application thereof. The ultrasonic activateable medicine-release albumin nanoparticles adopt azo-group-containing small molecules as a chemical cross-linking agent and albumin as a nanoparticle framework, coats an anti-cancer medicine and is prepared by using a solvent removing method; and the nanoparticles have particle sizes of 50-300nm under normal physiological conditions, and are uniform and stable in distribution. Under the action of ultrasonic waves acceptable by aliving body, the azo bond of the cross-linking agent can be broken, then the structure of the albumin nanoparticles can be damaged, medicines coated by the albumin nanoparticles can be rapidly released, the release amount of the medicines can be regulated and controlled through ultrasonic time and intensities, and the maximum release amount is up to 70%. Through intensive permeability of the ultrasonic waves, the albumin nanoparticles are capable of achieving rapid and controlled release of medicines at tumor tissue.

The present invention belongs to the field of bio-pharmaceutical material preparation technology, and relates to a preparation method of albumin nanoparticle carrier entrapping taxane-typed drug. This preparation method rapidly forms nanoparticle solution of albumin entrapping taxane-typed drug under room temperature by adding solvent as the medium. Next, by the second time of freeze-drying, stable powder of albumin nanoparticles entrapping taxane-typed drug is obtained. The final freeze-dried powder only includes two components: albumin and taxane-typed drug. The particles are in regular spherical shape, and the diameter of particle is less than 100 nm. The present invention has high drug loading ratio and entrapment efficiency. The experiment of releasing in vitro shows that the present invention has a good slow-release effect. Taxane-typed drug nanoparticles provided by the present invention improves the safety and compliance of this type of reagent.