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Preparation method of novel pesticide bovine serum albumin-thiacloprid nanoparticles

A bovine serum albumin and nanoparticle technology, which is applied in the field of preparation of new-type pesticide bovine serum albumin-thiacloprid nanoparticles, can solve the problems of difficult transportation, difficult long-term storage, obvious burst release phenomenon, etc. The effect of lower equipment requirements and lower manufacturing costs

Active Publication Date: 2017-05-31
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there are various thiacloprid dosage forms on the market, such as powder, wettable powder, granule, water suspension, microcapsule powder, microcapsule water suspension, etc., all of which have certain transportation difficulties and obvious burst release phenomenon. Not easy to store for a long time and many other problems

Method used

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  • Preparation method of novel pesticide bovine serum albumin-thiacloprid nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Take 0.08g of bovine serum albumin in 4ml of double distilled water to prepare the water phase, 50 o C water bath 30min, obtain clear and transparent bovine serum albumin aqueous solution;

[0026] (2) Take another 1mg of thiacloprid, add 0.167ml of acetonitrile for ultrasonic dissolution for 5min, and prepare 6mg / mL thiacloprid-acetonitrile oil phase;

[0027] (3) Adjust the speed of the water phase to 1000rpm, add the thiacloprid-acetonitrile oil phase drop by drop under high-speed stirring, emulsify for 30min, and take out the emulsion quickly within 0-4 o Cool at 10°C for 10min, then take out the ultrasonic wave for 10min to obtain bovine serum albumin-thiacloprid supermicroemulsion;

[0028] (4) Dialyze the supermicroemulsion dialysis membrane (MWCO=5000) overnight, take out the dialysate, freeze it, and dry it to obtain a white powder, that is, thiacloprid albumin nanoparticles.

Embodiment 2

[0030] (1) Take 0.08g of bovine serum albumin in 4ml of double distilled water to prepare the water phase, 50 o C water bath 30min, obtain clear and transparent bovine serum albumin aqueous solution;

[0031] (2) Take another 2 mg of thiacloprid, add 0.334 ml of acetonitrile for ultrasonic dissolution for 5 minutes, and prepare 6 mg / mL thiacloprid-acetonitrile oil phase;

[0032] (3) Adjust the rotation speed of the water phase to 1000rpm, add the thiacloprid-acetonitrile oil phase dropwise under high-speed stirring, emulsify for 40min, and take out the emulsion quickly within 0-4 o Cool at 10°C for 10min, then take out the ultrasonic wave for 10min to obtain bovine serum albumin-thiacloprid supermicroemulsion;

[0033] (4) Dialyze the supermicroemulsion dialysis membrane (MWCO=5000) overnight, take out the dialysate, freeze it, and dry it to obtain a white powder, that is, thiacloprid albumin nanoparticles.

Embodiment 3

[0035] (1) Take 0.08g of bovine serum albumin in 4ml of double distilled water to prepare the water phase, 50 o C water bath 30min, obtain clear and transparent bovine serum albumin aqueous solution;

[0036] (2) Take another 3 mg of thiacloprid, add 0.501 ml of acetonitrile to ultrasonically dissolve for 10 min, and prepare 6 mg / mL thiacloprid-acetonitrile oil phase;

[0037] (3) Adjust the rotation speed of the water phase to 1200rpm, add the thiacloprid-acetonitrile oil phase drop by drop under high-speed stirring, emulsify for 40min, and take out the emulsion quickly within 0-4 o Cooling for 5min under C, then take out the ultrasonic wave for 10min to obtain bovine serum albumin-thiacloprid supermicroemulsion;

[0038] (4) Dialyze the supermicroemulsion dialysis membrane (MWCO=5000) overnight, take out the dialysate, freeze it, and dry it to obtain a white powder, that is, thiacloprid albumin nanoparticles.

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Abstract

The invention discloses a preparation method of novel bovine serum albumin covered thiacloprid pesticide nanoparticles. An emulsifying-curing method is adopted in a process of covering thiacloprid with bovine serum albumin; the preparation method comprises the following steps: firstly, dissolving a certain amount of the bovine serum albumin into double distilled water to prepare a water phase; dissolving the thiacloprid into acetonitrile to obtain an oil phase; stirring at a high speed and dropwise adding the oil phase into the water phase; emulsifying for a period of time and rapidly cooling; carrying out ultrasonic crushing to obtain super-micro emulsion; freezing and coldly drying to obtain thiacloprid albumin nanoparticles. According to a novel albumin nanometer preparation technology adopted by the method, pesticide is loaded into albumin under the condition that the structure of the albumin is not changed and the pesticide loading amount is increased; meanwhile, a product has a slow releasing function. A nanotechnology is adopted so that a pesticide product is miniaturized, the resource waste is reduced, the cost is reduced and the pesticide effect is remarkably improved; the contact area between the pesticide and target insects is effectively enlarged and the pesticide is more easily swallowed and absorbed; contact toxicity and stomach poisoning actions are expressed better; high efficiency, low toxicity and safety of nano biological pesticide are realized.

Description

technical field [0001] The invention discloses a method for preparing novel pesticide bovine serum albumin-thiacloprid nanoparticles, in particular, the process of bovine serum albumin wrapping thiacloprid adopts an emulsification and solidification method. The invention belongs to the technical field of nanomaterials and new pesticides. Background technique [0002] Thiacloprid is another new type of chloronicotinoid insecticide developed by Bayer after imidacloprid, the chemical name is (3-((6-chloro-3-pyridyl)methyl)-1,3-thiazole Phenyl-2-ylidene) cyanamide has high efficiency, low toxicity, and broad-spectrum effects, and simultaneously has stomach toxicity, contact and systemic activity, and is an efficient agent for preventing and controlling sucking and chewing mouthparts pests. Its mechanism of action is similar to that of imidacloprid. It mainly acts on the post-junction membrane of insect nerves. By binding to nicotinic acid acetylcholine receptors, it interferes ...

Claims

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Application Information

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IPC IPC(8): A01N25/26A01N25/10A01N47/40A01P7/04
CPCA01N25/10A01N25/26A01N47/40
Inventor 温俊宝季英超刘彦雪雷建都李晓敏郑丹田雅洁
Owner BEIJING FORESTRY UNIVERSITY
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