Preparation method of material with nitric oxide (NO) catalytic activity
A technology of catalytically active materials and nitric oxide, which is applied in the direction of medical preparations, dental preparations, and pharmaceutical formulations of non-active ingredients, can solve the problem of no strong binding sites between NO catalytically active materials and material substrates, and it is difficult to maintain NO catalytic release , catalytic release of small amount of NO, etc., to achieve the effect of inhibiting smooth muscle cell proliferation, promoting endothelial cell adhesion, and inhibiting atherosclerosis
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Embodiment 1
[0022] A preparation method of a material with nitric oxide (NO) catalytic activity, the steps are:
[0023] A, the dopa (Dopa) that concentration is 1mg / mL, the selenocystamine of 1mg / mL and the cupric chloride that concentration is 1mg / mL are dissolved in the PBS buffer solution of pH=5-12, react at room temperature 12 hours.
[0024] B. The reaction solution in step A is centrifuged, washed, dialyzed and freeze-dried to obtain the target nitric oxide (NO) catalytically active material
Embodiment 2
[0026] A preparation method of a material with nitric oxide (NO) catalytic activity, the steps are:
[0027] A. Dissolve Dopamine (Dopamine) with a concentration of 2mg / mL, selenocystamine of 2mg / mL and copper chloride with a concentration of 2mg / mL in Tris buffer solution of pH=5-14, and react at room temperature for 24 Hour.
[0028] B. The reaction solution in step A is centrifuged, washed, dialyzed and freeze-dried to obtain the target nitric oxide (NO) catalytically active material
Embodiment 3
[0030]A preparation method of a material with nitric oxide (NO) catalytic activity, the steps are:
[0031] A. Dissolve norepinephrine with a concentration of 10mg / mL, selenocystamine with a concentration of 10mg / mL and copper chloride with a concentration of 10mg / mL in PBS buffer solution of pH=5-12, and react at room temperature for 12 Hour.
[0032] B. The reaction solution in step A is centrifuged, washed, dialyzed and freeze-dried to obtain the target nitric oxide (NO) catalytically active material
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