136 results about "Peptide modification" patented technology

The disclosed invention relates to methods of modifying peptide compositions to increase stability and delivery efficiency. Specifically, the disclosed invention relates to methods to increase the stability and delivery efficiency of protein kinase C (PKC) modulatory peptide compositions. A “therapeutic peptide composition” comprises a “carrier peptide” and a “cargo peptide.” A “carrier peptide” is a peptide or amino acid sequence within a peptide that facilitates the cellular uptake of the therapeutic peptide composition. The “cargo peptide” is a PKC modulatory peptide. Peptide modifications to either the carrier peptide, the cargo peptide, or both, which are described herein increase the stability and delivery efficiency of therapeutic peptide compositions by reducing disulfide bond exchange, physical stability, reducing proteolytic degradation, and increasing efficiency of cellular uptake.

The invention relates to preparation and application of a nano-micelle imaging agent for a cervical cancer sentinel node. A nanometer imaging agent high polymer PEG-PLGA is used as a carrier, micelleparticles are formed through polymerization, indocyanine green is loaded inside, TMTP1 targeting peptide is modified on the surface of a micelle, and maleic amide connected with a surface hydrophilicgroup PEG end of the micelle is connected with a sulfhydryl group of TMTP1 with ring formation by amido bonds at the head and the tail. The near infrared fluorescence nano-micelle diagnosis reagent provided by the invention can image the cervical cancer sentinel node in real time, can distinguish whether tumor cell metastasis occurs or not, and offers the assistance for the imaging diagnosis of intraoperative lymph nodes of cervical cancer in clinic in the future and the guidance of individualized treatment of tumor patients.

The invention discloses an application of a transdermal peptide modified pueraria thomsonii exosome nano preparation in preparation of an anti-skin aging product. The pueraria thomsonii exosome nano preparation modified by the transdermal peptide can effectively penetrate through the cuticle of the skin and is efficiently taken by skin cells such as skin fibroblasts, so that the obvious function of resisting skin (cell) aging is exerted, and a new strategy is provided for skin aging resistance; The transdermal peptide modified pueraria thomsonii exosome can be used as a novel skin anti-aging beauty product which is efficient, safe and convenient to prepare, can also be applied to aging resistance of other cells and tissues, and is used for delaying aging and function decline of body tissues and organs; therefore, great market development prospects are realized in the fields of medical products and beauty cosmetics.

The invention discloses a preparation method and application of a targeting peptide modified traditional Chinese medicine multi-component exosome-like fusion nanoparticle, the nanoparticle is mainly formed by fusing a functionalized liposome and an exosome, the functionalized liposome is mainly composed of a targeted peptide, phospholipid and a traditional Chinese medicine fat-soluble active drug, the exosome is loaded with a traditional Chinese medicine water-soluble active medicine. The preparation method of the fusion nanoparticles comprises a polyethylene glycol induction method, a repeated freezing and thawing method, an extrusion method, an ultrasonication method and an incubation method. The preparation process is simple, the condition is mild, the cost is low, and the prepared nanoparticles have the advantages of various drug loading types, high penetrability, biological targeting regulation, therapeutic mechanism complementation, biological safety and the like. According to the nano-drug delivery platform, co-delivery, combined administration and collaborative precise treatment of multiple components of traditional Chinese medicine can be achieved, collaborative targeted treatment of complex progressive diseases such as tumors and neurodegenerative diseases can be achieved, and the nano-drug delivery platform has good application prospects.

The invention discloses 1-(4-isopropyl propyl)-beta-carboline-3-formyl-Trp-Trp-Trp-Lys-Glu structured as follows, a preparation method therefor, a nanostructure thereof, antitumor action thereof, action thereof in resisting adhesion and invasion and migration of tumor cells, and illustrates application thereof in medical science. The formula is shown in the description.

The invention discloses a preparation method and application of a high-density RGD peptide modified material. The preparation method comprises the following steps: dispersing a material, modified in the way that the surface is provided with an active group, into methylbenzene, adding a catalytic amount of 4-dimethylamino-pyridine, and slowly dropwise adding triethylamine and 2-bromoisobutyryl bromide under ice-bath, so as to prepare an initiator; adding cuprous bromide, 2,2'-dipyridyl and poly(ethylene glycol) methyl ether methacrylate into the initiator, so as to obtain a macromolecular polymer; adding the macromolecular polymer into a dimethylformamide solution containing an activating agent and 4-dimethylamino-pyridine to obtain an activated polymer brush, and adding RGD peptide for performing reaction in the dark at the room temperature for 18 hours; transferring a cell into the RGD peptide modified polymer brush, and performing reaction on a shaking table at the temperature of 4 DEG C for 20 minutes by adopting a phosphate buffer solution as a suspension liquid, so as to obtain the high-density RGD peptide modified material. The method is moderate in reaction conditions, simple, convenient and feasible in procedures, and achieves the purpose of fixing a cell on the surface of a solid material with high concentration and high density.

The invention provides an adriamycin-carrying lipid nanoscale ultrasound contrast agent targeting tumor-related fibroblasts and a preparation method of the adriamycin-carrying lipid nanoscale ultrasound contrast agent. The surface of the contrast agent is modified by a high affinity ligand (FH short peptide) for specifically expressing a TNC protein by the tumor-related fibroblasts, the FH short peptide is used as a targeting ligand, lipid is used as a shell membrane material, and adriamycin and gaseous fluorocarbon are wrapped inside the shell membrane. The targeted ultrasound contrast agentis in nanoscale, has ultrasound imaging capability, can be used for targeted delivery of drugs into the tumor-related fibroblasts, and specifically kills the tumor-related fibroblasts, and a tumor treatment effect is achieved through intervention on a tumor microenvironment.

The present invention relates to peptide modifier compounds of Formula (1), or a salt thereof, wherein: a is an integer from 1 to 10, more preferably from 1 to 3; b is an integer from 0 to 7; Z is a terminal group and Y is a bivalent group. Further aspects of the invention relate to intermediates in the preparation of compounds of Formula (1), and the use of compounds of Formula 1 in the synthesis of peptide derivatives.

The invention relates to a specific peptide modified colorimetric sensor and a making method thereof. The colorimetric sensor is used for detecting copper ions. The sensor uses a gold-mercapto key to make the surface of gold nanoparticles to be modified by a specific peptide, the addition of copper ions to be measured changes the conformation of the specific polypeptide from alpha-helix to beta-folding, and the gold nanoparticles mutually aggregate by using beta-folding fragments formed by the surface modification specific polypeptide in order to change the absorbance of a solution and generate color difference. The copper ions are detected by the gold nanoparticles modified by the specific peptide for the first time, and the colorimetric sensor of the gold nanoparticles modified by the specific polypeptide has very high sensitivity, simplicity and specificity on the copper ions, has a wide detection range, has potential application values in the detection of the content of the copper ions in drinking water, serum, rivers, sewage and other solutions, and also has the advantages of convenient operation, high sensitivity and wide application prospect.

The invention provides a mesenchymal stem cell exosome agent based on a DNA tetrahedron, a preparation method and application of the mesenchymal stem cell exosome agent. The exosome preparation is an exosome agent Exo-TDN which is obtained by loading a DNA tetrahedral nano material on the surface of a mesenchymal stem cell exosome; or the exosome agent is an exosome preparation PCM-Exo-TDN which is obtained by loading a DNA tetrahedral nano material on a mesenchymal stem cell exosome and modifying a myocardial specific targeting peptide PCM on the surface of the exosome through a cross-linking agent phospholipid polyethylene glycol maleimide. Exo-TDN is mainly enriched in a liver area, the chemotherapeutic drug DOX can be captured by the Exo-TDN when reaching the liver, the DOX is inhibited from entering a cell nucleus, and the function of protecting cells is achieved; a myocardial specific targeting peptide PCM is further modified, and PCM-Exo-TDN is synthesized and used for relieving myocardial damage caused by DOX.