The invention discloses an acrylic polyester ramie cotton mixed fabric pretreatment process which comprises the following steps: turning cloth, sewing the end, singeing, desizing-scouring, bleaching, drying, mercerizing and drying, wherein the singeing step refers to one-step singeing, the four burners refer to two positive burners and two reverse burners, and the speed is 20m per minute; according to the desizing-scouring step, the caustic soda concentration in a working solution is 16g / L; according to the bleaching step, 6-8g / L of hydrogen peroxide (100 percent) is adopted, 2g / L of a stabilizer EDTMP is adopted, cold rolling is performed under the pH value of 10-10.5, and steaming is performed at the temperature between 90 DEG C and 95 DEG C for 60 minutes; and the concentration of alkali in a mercerizing solution in the mercerizing step is 140-160g / L. The singeing effect achieves a level 4; the desizing and scouring are combined into a process, the action time of acrylic fibers and strong base is reduced, and impurities on ramie cotton are effectively removed; and moreover, the acrylic polyester is not yellowed, and high strength is maintained.