32results about How to "Rapid Analysis" patented technology

The present invention provides a low cost imaged-based system for detecting, measuring and / or counting labeled features of biological samples, particularly blood specimens. In one aspect, the invention includes a system for imaging multiple features of a specimen that includes one or more light sources capable of successively generating illumination beams each having a distinct wavelength band and a plurality of differentially excitable labels capable of labeling a specimen comprising multiple features, such that each different feature is labeled with a different differentially excitable label. System of the invention may further include a controller operationally associated with the one or more light sources for successively directing illumination beams onto the specimen so that each of the different differentially excitable labels is successively caused to emit an optical signal within the same wavelength band, an optical system capable of collecting such emitted optical signals and forming successive images corresponding to the labeled features of the specimen on a light-responsive surface to form successive sets of image data thereof, and a disposable cuvette for collection and optical analysis of non-red blood cells.

A biomolecule analysis kit includes a reaction container configured to perform an enzymatic reaction, the reaction container including a base portion which has a container-shaped portion and a low-adsorption structural portion which is provided on at least the inner surface of the container-shaped portion, the low-adsorption structural portion having an adsorption rate lower than the base portion at which at least one of a sample which becomes a target of analysis in the enzymatic reaction and a reagent for the enzymatic reaction is adsorbed thereonto, wherein a signal resulting from the enzymatic reaction is configured to be detected when the enzymatic reaction is performed in the reaction container.

The invention provides a method for measuring liquid phase ozone concentration by flow injection ozonation, which solves the problems of measurement result error caused by selection of traditional trapping agents and interference of metal ions in matrix water samples, and secondary pollution in the prior art. The method comprises the following steps: (1) conveying an ozone solution to be detected through a pump; (2) mixing the ozone solution with a tertiary butanol solution in a tertiary butanol pipeline; (3) mixing with a luminol solution in a luminol pipeline and a metal-chelator solution in a metal-chelator solution pipeline, detecting chemiluminescent signals generated by ozone molecules and luminol in the mixed solution by a photomultiplier; (4) collecting and amplifying the optical signals, converting into electric signals, sending the electric signals into a computer data processing system, calculating the liquid phase ozone concentration, displaying, and printing for output. The tertiary butanol is adopted to eliminate interference on luminol ozonation caused by traditional trapping agents; the metal-chelator is adopted for complexation with metal ions in the water, and the generated metal complex does not catalyze chemiluminescence of luminol.

The invention provides a method for measuring the dissolved oxygen of a water body by a flow-injection chemiluminiscence mode. The method comprises the following steps: (1) a water sample pump conveys measured water body solution; (2) the water body solution is mixed with hydrogen peroxide solution; (3) the solution mixed with hydrogen peroxide is mixed with acid buffer solution, and then enters a cooling pipe; (4) the mixed solution flows out of the cooling pipe, is mixed with luminal solution and alkaline buffer solution in an alkaline buffer solution pipeline and flows into a detection room; and (5) a photomultiplier acquires and amplifies light signals emitted by passed solution, converts the light signals into electric signals, and conveys the electric signals into a microcomputer data processing system, and the microcomputer data processing system quantizes the signals, and calculates the concentration of the dissolved oxygen in the water body. The method provided by the invention has the advantages of high sensitivity, wide linear range, fastness, good reproducibility, high automation degree and high accuracy and the like.

The invention provides a detecting device and method for detecting flow injection chemiluminescence water inorganic mercury by inhibiting, aims to solve the problems that in the prior art, analyzing time is long, conditions are rigorous, energy consumption is high, secondary pollution can be produced, concentration of ozone is unstable and lighting efficiency is low and the like. According to the device, hydrophobic transparent material is coated on on the upper portion on the inner face of a dynamic ozone solution balancing pipe close to the face of a ultraviolet tube, and a plurality of reflectors are arranged on the lower portion. By the aid of the scheme, dissolved ozone is precipitated rarely when the ozone solution flows through a dynamic ozone solution balancing chamber, stability of ozone concentration is guaranteed, and lights are reflected to the solution through the prismatic reflectors to improve ozone-dissolving lighting efficiency. The detecting device used for detecting the inorganic mercury in water has the advantages of scene, rapidness, convenience and flexibility.

The present invention presents construction of a detection method requiring no step of removing free biotin during preparation of a biotinylated protein having a biotin tag, in a detection method of a substance interacting with a protein, and studied various preparation methods of the biotinylated protein. In order to solve the above-mentioned problem, the present inventor has found that in a cell-free protein synthesizing system, in particular, a wheat embryo cell-free protein synthesizing system, when biotinylation is performed during or after protein's synthesis, the biotinylation of the protein can be attained in an remarkably lower concentration of the biotin than that in the conventional biotinylation operations, and has accomplished the present invention by using the protein having the biotin tag in each detection system.

The invention discloses a method for detecting aflatoxin B1 by enzyme-labeled aptamer. The method sequentially includes the steps: (1) taking a coated microporous plate, adding a sample to be detected and a specific probe for co-incubation and then washing the microporous plate; (2) detecting the aflatoxin B1 by detecting corresponding signals of the specific probe. The coated microporous plate is a microporous plate coated with aflatoxin B1-BSA conjugates, the specific probe is a compound of a first element and a second element, the first element is a substance obtained by connecting biotin to the tail end of the aptamer specifically combined with the aflatoxin B1 by the aid of triethylene glycol serving as a connecting arm, and the second element is horseradish peroxidase-labeled streptavidin. The enzyme-linked aptamer analysis method has the advantages of simplicity, rapidness and high sensitivity, a plurality of samples can be rapidly analyzed, a detection kit can be made, and the method has a great application prospect in AFB1 analysis detection.

The invention provides a detecting device and method for detecting flow injection chemiluminescence water inorganic mercury, aims to solve the problems that in the prior art, an ozone solution is easy to decompose, on-site operation cannot be performed, analyzing time is long, analyzing process is complicated, conditions are rigorous, energy consumption is high, secondary pollution can be produced and the like. A catalyst of ruthenium trichloride is filled into a dynamic ozone solution balancing pipe; when the ozone solution flows into the dynamic ozone solution balancing pipe, the catalyst of the ruthenium trichloride is irradiated by a fluorescent light, the ozone in the ozone solution is inhibited from generating hydroxyl radicals, the ozone solution is stable and decompose rarely, and stability of the ozone solution is improved. The detecting device used for detecting the inorganic mercury in water has the advantages of scene, rapidness, convenience and flexibility.

The invention discloses a device for measuring zinc in water by virtue of flow injection spectrophotometry and a method for measuring zinc in water, wherein the device has relatively high sensitivity and good selectivity and can be directly applied to determining trace zinc in environmental water. The device is characterized by being composed of a water sample pump (3), a buffer solution pump (5), a chelating agent solution pump (7), a masking column (8), a first complexing agent pump (10), an eluent pump (12), an enrichment-elution column (14), a buffer solution pump (16), a second complexing agent pump (18) and a detection chamber (22), wherein the water sample pump (3), the buffer solution pump (5) and the chelating agent solution pump (7) are connected with the inlet end of the masking column (8) by use of pipelines; the first complexing agent pump (10) and the eluent pump (12) are connected with the outlet end of the masking column (8) by use of pipelines; the eluent pump (12) is connected with the inlet end of the enrichment-elution column (14) by use of a three-way valve; and the buffer solution pump (16), the second complexing agent pump (18) and the detection chamber (22) are connected with the outlet end of the enrichment-elution column (14). The invention also discloses a method for measuring zinc in water.

The invention provides a method for measuring a water sulfide concentration through flow injection chemiluminescence. The method is performed according to the following steps of: 1. inputting a measured water sample into a water sample reaction chamber in a reaction chamber; 2. delivering ozone into the water sample reaction chamber in the reaction chamber, performing a gas-liquid phase reaction for 30-50s, carrying out ozone oxidation to the sulfide in the water sample so as to generate sulfur dioxide; 3. transporting the completely reacted measured water sample solution, which is mixed with phosphoric acid in a phosphoric acid pipeline; 4. then mixing the mixed solution mixed with the phosphoric acid with a potassium permanganate solution, and letting a photomultiplier tube detect a chemiluminescent signal generated during oxidation of the sulfur dioxide by potassium permanganate in the solution; and 5. allowing the photomultiplier tube to collect and amplify an optical signal emitted by a solution flowing through, converting the signal into an electric signal, which is then sent into a microcomputer data processing system, then quantizing the signal, and calculating the concentration of the sulfide in the water. The invention has the advantages of high sensitivity, wide linear range, rapidity, good reproducibility, as well as high degree of automation, etc.

The invention discloses a method for simultaneously determining arsenic forms of roxarsone, arsanilic acid and degradation products of same in soil, which comprises the following steps: adding a mixedextracting solution of NaH2PO4 and H3PO4 in a volume ratio of 9: 1 into a soil sample, uniformly mixing same, heating and extracting in a microwave digestion instrument, and centrifugally filtering the obtained extracting solution for high performance liquid chromatography-inductively coupled plasma-tandem mass spectrometry determination. The arsenic forms of roxarsone, arsanilic acid and degradation products of the same in the soil can be simultaneously and quickly determined; the determination accuracy, precision and stability all meet the requirements of environmental residue analysis anddetection; an adding standard recovery rate of roxarsone, arsanilic acid, monomethyl arsenic, dimethyl arsenic and inorganic arsenic ranges from 73.4% to 105.0%; the instrument detection limit rangesfrom 0.85 to 1.59 microgram / L, the precision ranges from 1.7% to 3.8%, and the requirement for polluted soil detection is met.

The invention provides a device and a method for measuring a cadmium element in water through liquid chromatogram flow injection chemiluminescence. According to the method, a complex is formed based on cadmium in the water and methyl glycol biacrylate, chemiluminescence is generated by the complex and ethyl acetoacetate in a cyclohexane organic phase system, a linear relationship exists between the chemiluminescence intensity and the concentration of the cadmium element in the water, and a method for measuring the cadmium in the water through the liquid chromatogram flow injection chemiluminescence is established by utilizing a liquid chromatographic separation flow injection technology. The method for measuring the cadmium element in the water has the characteristics of field measurement, rapidness, simplicity and sensitivity.

The invention provides a device and a method for measuring total chromium of a water body by a flow-injection chemiluminiscence method by enriching and eluting. The method comprises the following steps: enriching total chromium based on a magnetic nano material, then eluting, forming a complex by eluted total chromium and dimethyl dimethoxy silicane, and oxidizing the complex and peroxided oxalate so as to generate chemiluminiscence, wherein the chemiluminiscence intensity has linear relationship with the concentration of the total chromium in the water body. A method for measuring total chromium of the water body by the flow-injection chemiluminiscence method by enriching and eluting of the magnetic nano material is established by utilizing an enriching-eluting-complexing-chemiluminiscence technique. The characteristics of rapidity, simplicity, convenience and high accuracy can be achieved in the determination of total chromium in the water body.

Mismatch Repair Detection (MRD), a novel method for DNA-variation detection, utilizes bacteria to detect mismatches by a change in expression of a marker gene. DNA fragments to be screened for variation are cloned into two MRD plasmids, and bacteria are transformed with heteroduplexes of these constructs. Resulting colonies express the marker gene in the absence of a mismatch, and lack expression in the presence of a mismatch. MRD is capable of detecting a single mismatch within 10 kb of DNA. In addition, MRD can analyze many fragments simultaneously, offering a powerful method for high-throughput genotyping and mutation detection.

The present invention concerns a method of analyzing a cancer of a patient, in particular an adrenocortical carcinoma, on the basis of determining (i) chromosomal alterations identified specifically for said cancer and (ii) DNA methylation status of genes of said chromosome regions, in a single targeted NGS.

The invention provides a method for measuring a water sulfide concentration through flow injection chemiluminescence. The method is performed according to the following steps of: 1. inputting a measured water sample into a water sample reaction chamber in a reaction chamber; 2. delivering ozone into the water sample reaction chamber in the reaction chamber, performing a gas-liquid phase reaction for 30-50s, carrying out ozone oxidation to the sulfide in the water sample so as to generate sulfur dioxide; 3. transporting the completely reacted measured water sample solution, which is mixed with phosphoric acid in a phosphoric acid pipeline; 4. then mixing the mixed solution mixed with the phosphoric acid with a potassium permanganate solution, and letting a photomultiplier tube detect a chemiluminescent signal generated during oxidation of the sulfur dioxide by potassium permanganate in the solution; and 5. allowing the photomultiplier tube to collect and amplify an optical signal emitted by a solution flowing through, converting the signal into an electric signal, which is then sent into a microcomputer data processing system, then quantizing the signal, and calculating the concentration of the sulfide in the water. The invention has the advantages of high sensitivity, wide linear range, rapidity, good reproducibility, as well as high degree of automation, etc.

The invention provides a detecting device and method for detecting flow injection chemiluminescence water inorganic mercury, aims to solve the problems that in the prior art, an ozone solution is easy to decompose, on-site operation cannot be performed, analyzing time is long, analyzing process is complicated, conditions are rigorous, energy consumption is high, secondary pollution can be produced and the like. A catalyst of ruthenium trichloride is filled into a dynamic ozone solution balancing pipe; when the ozone solution flows into the dynamic ozone solution balancing pipe, the catalyst of the ruthenium trichloride is irradiated by a fluorescent light, the ozone in the ozone solution is inhibited from generating hydroxyl radicals, the ozone solution is stable and decompose rarely, and stability of the ozone solution is improved. The detecting device used for detecting the inorganic mercury in water has the advantages of scene, rapidness, convenience and flexibility.

The invention provides a device and a method used for measuring selenium in water bodies via molecular imprinting selective laser induced fluorescence. According to the device, a fluorescent pool is designed to be flat; the inner bottom of the fluorescent pool is coated with a molecularly imprinted polymer with absorption capacity on a complex formed from hydroxyethyl ethylenediamine triacetic acid and selenium IV in water bodies. According to the method, the complex is formed from selenium IV in water bodies and hydroxyethyl ethylenediamine triacetic acid, the molecularly imprinted polymer prepared by taking the selenium IV-hydroxyethyl ethylenediamine triacetic acid complex as a template molecule is capable of generating fluorescence at certain wave length under laser light source excitation, fluorescence signal strength and the concentration of selenium in water bodies are linearly associated, high efficiency selective enrichment is realized via molecular imprinting, and the method used for measuring selenium in water bodies is established via laser induced fluorescence method. When the device and the method are used for measuring selenium in water bodies, one-site detection can be realized, detection is rapid and simple, and sensitivity is high.

A method for measuring a concentration of a hydroperoxide of an alkylaromatic hydrocarbon in a process stream comprises immersing a probe into the process stream; wherein the probe is coupled to a spectrometer; collecting absorption data with the spectrometer at a wavelength of 13,000 cm<-1 >to 4,000 cm<-1>; and calculating a concentration of the hydroperoxide of the alkylaromatic hydrocarbon in the process stream. In another embodiment, samples are withdrawn from the process stream and analyzed spectrometrically to determine the concentration of the hydroperoxide of the alkylaromatic hydrocarbon in the sample.

The invention provides a device and a method for measuring lead in water by a flow injection fluorescence quenching method. The method is established by using a chelation-enrichment-quenching flow injection technology, wherein lead in a water body and polymethylacrylic acid form a metal chelate under the action of a catalyst, and the generated metal chelate has a quenching function on fluorescent light generated by fluorescein isothiocyanate; meanwhile, a fluorescence signal inhibition degree difference value and a concentration of the lead in the water body have a linear relation. According to the device and the method, a catalysis device with a temperature control function is adopted on the aspect of forming the metal chelate by the lead and the polymethylacrylic acid, and is a carbon nano tube internally loaded with an Ni-Cu-Al metal compound oxide, and the carbon nano tube is used as a carrier, and has a good supporting and dispersing function on a metal active part of the catalyst, so that the activity of the catalyst is greatly improved so that the efficiency of forming the metal chelate by the lead and the polymethylacrylic acid under a proper pH condition is maximal and stable.

The invention provides a device and a method for measuring total chromium of a water body by a flow-injection chemiluminiscence method by enriching and eluting. The method comprises the following steps: enriching total chromium based on a magnetic nano material, then eluting, forming a complex by eluted total chromium and dimethyl dimethoxy silicane, and oxidizing the complex and peroxided oxalate so as to generate chemiluminiscence, wherein the chemiluminiscence intensity has linear relationship with the concentration of the total chromium in the water body. A method for measuring total chromium of the water body by the flow-injection chemiluminiscence method by enriching and eluting of the magnetic nano material is established by utilizing an enriching-eluting-complexing-chemiluminiscence technique. The characteristics of rapidity, simplicity, convenience and high accuracy can be achieved in the determination of total chromium in the water body.

The invention relates to a vehicle separation determining and traffic flow surveying device and a corresponding determining and surveying method, and aims at solving the problems of high error rate and low recognizing speed of a vehicle traffic flow detection mode in the prior art. The device comprises a bar code area corresponding to road width, and a shooting and video recording detecting device which is arranged above the bar code area and covers the road width with the shooting range, wherein the shooting and video recording detecting device comprises a camera, a CCD linear sensor for receiving a light source of the camera and transmitting to a photoelectric conversion element, and a single-chip microcomputer which is in communication connection with the photoelectric conversion element; the bar code area comprises a certain quantity of stripy belts of which the width is a and the intervals range from 2a to 5a; and the bar code area forms zero stripes which are inclined by angle beta relative to the road direction. The device has the beneficial effects that the real-time drive-in and drive-out conditions of vehicles can be quickly analyzed through the special shooting and video recording detecting device and according to the shape of bar codes, and the data of forward moving or backward moving of the vehicle after stopping in a short time beside the detecting device can be determined; and a pavement is prevented from being damaged during the mounting process.

The invention discloses a device for measuring zinc in water by virtue of flow injection spectrophotometry and a method for measuring zinc in water, wherein the device has relatively high sensitivity and good selectivity and can be directly applied to determining trace zinc in environmental water. The device is characterized by being composed of a water sample pump (3), a buffer solution pump (5), a chelating agent solution pump (7), a masking column (8), a first complexing agent pump (10), an eluent pump (12), an enrichment-elution column (14), a buffer solution pump (16), a second complexing agent pump (18) and a detection chamber (22), wherein the water sample pump (3), the buffer solution pump (5) and the chelating agent solution pump (7) are connected with the inlet end of the masking column (8) by use of pipelines; the first complexing agent pump (10) and the eluent pump (12) are connected with the outlet end of the masking column (8) by use of pipelines; the eluent pump (12) is connected with the inlet end of the enrichment-elution column (14) by use of a three-way valve; and the buffer solution pump (16), the second complexing agent pump (18) and the detection chamber (22) are connected with the outlet end of the enrichment-elution column (14). The invention also discloses a method for measuring zinc in water.

Compositions, kits, and methods of using the kits and compositions to determine the hardness of and / or magnesium concentration in a solution are described. The kit can include a lyophilized composition that has an absorbance at a detectable wavelength in response to the hardness of the solution and or the concentration of magnesium in the solution.

The invention discloses a method for detecting unqualified fish meat raw materials used in prepackaged products, comprising the following steps: step a, selecting a sample to be tested; step b, preparing a sample liquid to be tested; step c, detecting the first sample liquid to be tested; d, the first standard curve is prepared; step e, the second sample liquid to be tested is detected; step f, the second standard curve is prepared; step g, the result is determined. The analysis method of this detection method is simple, rapid, highly specific, high sensitivity, obvious phenomenon, does not need large analytical instruments, does not need to use dangerous reagents such as highly toxic or carcinogenic, and has low harm to the experimental environment and personnel. Whether the raw materials used for packaging fish meat products are qualified, whether it is the fish meat with ammonia nitrogen poisoning or corrupted and stale fish meat, it is convenient to trace the source of the problem products and facilitate the daily supervision and management of prepackaged fish meat products.

The invention provides a method for measuring polycyclic aromatic hydrocarbon (PAHs) of sea water in a flow injection chemiluminescence way. The method adopts the scheme that the PAHs of the sea water is quickly enriched and eluted in an enrichment and elution way; and a chemiluminescence signal is detected via a chemiluminescence system formed by super oxidized oxalate, hydrogen peroxide and the PAHs, so that the method for measuring the PAHs of the sea water in the flow injection chemiluminescence way is achieved. The method for measuring the PAHs of the sea water has characteristics of site operation, speediness, simplicity and sensitivity.

The invention discloses a method for measuring DDT in water by molecular imprinting selective fluorescence quenching method. First, sodium ethylenediaminetetramethylene phosphate forms a complex with DDT in water samples, and then enriches the complexes sequentially. , eluted, and finally detected by fluorescence technology. The formed complex has an obvious quenching effect on the fluorescence system, and the degree of fluorescence signal quenching has a linear relationship with the concentration of DDT in water. The method has the characteristics of being on-site, fast, simple and sensitive, and solves the problems existing in the prior art such as inability to work on-site, long analysis duration, complicated analysis process, harsh conditions, high energy consumption, and especially secondary pollution.