35 results about "Roxarsone" patented technology

The invention belongs to the technical field of material chemistry, and discloses a molecular imprinting polymer for roxarsone detection, and a preparation method thereof. The molecular imprinting polymer preparation method comprises the following steps: carrying out polymerization of a template, a pore forming agent, a functional monomer, a cross-linking agent and an initiator for 8-48 h at a temperature of 50-80 DEG C, and removing the template to obtain the molecular imprinting polymer for roxarsone detection. According to the present invention, a roxarsone recovery rate of the prepared molecular imprinting polymer is more than 90%, and the prepared molecular imprinting polymer shows a high roxarsone cross reaction, has high selectivity and high specificity, and provides broad application prospects in the field of application of the prepared molecular imprinting polymer as a sample purification pretreatment material for roxarsone analysis in feeds, animal tissues, environment water and other matrixes.

The invention discloses a method for separating roxarsone and its degradation product in broiler manure. The method comprises 1, carrying out extraction on a manure sample through a methanol-water extraction agent, 2, condensing and purifying the sample through a solid phase extraction column and 3, determining a roxarsone recovery rate of the manure sample through an external standard method. The separation method utilizes the methanol-water extraction agent with a methanol-water volume ratio of 1: 9 to extract roxarsone residues in the manure sample and has a high recovery rate. When the sample is further purified through a solid phase extraction column, a formic acid-methanol-water agent with a formic acid-methanol-water volume ratio of 5: 65-75: 20-30 is used and an eluant with pH of 2 is used so that an ideal recovery rate is guaranteed and polar compound impurities in the sample can be removed and thus background interference in mass spectrum detection is reduced. The recovery rate of the separation method satisfies later detection requirements.

The invention discloses a double detection test paper for roxarsone and nifedipine, the test paper includes anti-roxarsone monoclonal antibody and anti-nifepine acid monoclonal antibody, and can simultaneously detect roxarsone and nifedipine Arsine. In the preparation of the antibody, the present invention adopts roxarsone (ROX) and nifexarsine (NIT) as haptens respectively, and transforms the nitro group into an amino group after reduction, and then couples with the amino group of the carrier protein to develop the roxarsone The artificial antigens of sarsenazo and nifedipine acid, the immunogens ROX‑BSA and NIT‑BSA have better immunogenicity. The obtained two kinds of monoclonal antibodies have the advantages of high specificity, high affinity and high sensitivity, which lay the foundation for the preparation of roxarsone and nifedipine acid dual detection test paper. Compared with the existing high-performance liquid chromatography detection technology, the immunochromatography test paper of the present invention has the advantages of simplicity, speed, sensitivity, accuracy, high throughput, etc., and can realize on-site real-time detection.

The invention discloses a preparation method of an iron-manganese oxide / starch / biochar composite material for simultaneously adsorbing inorganic arsenic and organic arsenic in a water body. Including the following steps: adding cornstarch and biochar to FeSO 4 and MnSO 4 solution, add KMnO under magnetic stirring 4 The solution was adjusted to pH 2.0 with NaOH, aged, filtered and dried to obtain the iron manganese oxide / starch / biochar composite. The addition of starch and biochar makes the synthesized iron-manganese oxide particles evenly dispersed and uniform in particle size, with a particle size range of 300nm-600nm, thereby significantly increasing the adsorption capacity of the composite material for trivalent arsenic, pentavalent arsenic and roxarsone . The surface active adsorption site of the material is Fe-OH group, which can form inner spherical Fe-O-As complex with arsenic compound. The preparation process of the invention is simple, the cost is low, the raw materials used are low-carbon and environmentally friendly, and various forms of arsenic in the water body can be adsorbed simultaneously.

The invention relates to an organic arsenic feed additive discharge pre-alarming monitoring method in a water environment system, belonging to the environmental monitoring technical field, comprising the following steps: preparing tetrahymena culture medium; preparing arsanilic acid standard sample solution / roxarsone standard sample solution; collecting the actual water sample and separating and extracting the organic arsenic feed additive compound; inoculating the tetrahymena into the prepared culture medium and then subpackaged the inoculated culture medium into the sample culture tubes; respectively adding the organic arsenic feed additive standard solution into the sample culture tubes and respectively measuring the corresponding tetrahymena cell density using absorptometry; making the actual water sample intervening the corresponding tetrahymena biological model and analyzing and determining the corresponding tetrahymena cell density; based on the comparison of the tetrahymena cell density and the contrast of the corresponding tetrahymena cell density after the actual water sample intervention, to obtain the concentration range of the pollutant presence, thereby pre-alarming the discharge condition of the organic arsenic feed additive in the corresponding actual water environment.

The invention discloses a method for rapidly detecting arsanilic acid and roxarsone in water sample. The method comprises the following specific steps of: firstly taking three aliquots of identical water samples, respectively adjusting the pH values of the two aliquots of water samples to be strongly acidic or strongly alkali, then pouring the two aliquots of water samples with adjusted pH values into a quartz reactor, leading into inert gas for removing oxygen, switching on an ultraviolet disinfection lamp for irradiation so as to convert organic selenium into inorganic selenium; and after the reaction is finished, detecting inorganic selenium contained in the three aliquots of water samples by adopting an atomic fluorescence spectroscopy, and respectively obtain background value of inorganic selenium, content of arsanilic acid and content of roxarsone in the water samples. The method is simple to operate, good in repeatability, and high in selectivity, the detection limit of the organic selenium can be greatly reduced, the detection precision can be improved, and the detection capability of a laboratory can be effectively promoted; in addition, chemical reagents are common reagents, so that the cost is low, the use amount is small, and the induced secondary pollution is small.

The invention discloses a method for extracting 3-nitryl-4--hydroxyphenylarsonic acid from waste water comprising Roxarsone: the waste water comprising Roxarsone is mixed with an organic solvent A for extracting under 25-75 DEG C under the effect of an extracting agent A to obtain a primary water course, a primary organic solvent course and a primary extracting agent course. The primary extracting agent course is added into aqueous alkali A and water A and stirred to adjust a pH value to 8-14 and then stewed for delamination to obtain a final water course and a final extracting agent course; the pH value of the final water course is adjusted to 0.5-2 and then the final water course is crystallized under 30-75 DEG C, and filtered after the final water course is completely crystallized to obtain filter liquor and filter cake; and the filter cake is dried to obtain the 3-nitryl-4--hydroxyphenylarsonic acid. The method is simple to operate and has less equipment investment and low energy consumption. The solvent and extracting agent used can be recycled and reused and have less loss; therefore, the material input is low. Moreover, not wastes are generated; therefore, the method is clean and environmental friendly.