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Emission early-warning monitoring method for organic arsenic feed additive in water environment system

A feed additive, early warning and monitoring technology, applied in the preparation of test samples, color/spectral characteristic measurement, etc., can solve the problems of high cost of reagents, harsh conditions of experimental equipment, etc.

Inactive Publication Date: 2010-12-29
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of reagents required for the cultivation of human cells is high, and the conditions of experimental equipment are harsh. At the same time, comparing the results of the growth of corresponding cells with the control group, it is not possible to scientifically monitor and monitor organic substances such as roxarsarsenic from the perspective of environmental ecological food chain cycle. Emission of Arsenic Pollutants in Aquatic Environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0025] (1) culture medium preparation;

[0026] Weigh 10g of peptone and 2g of glucose, dissolve them in 1000mL of deionized water, stir evenly, distribute them in 150mL Erlenmeyer flasks (each bottle has 80mL medium), sterilize in an autoclave at 121°C for 20-30min, and then statically Bring to room temperature and store in a refrigerator at 4°C.

[0027] (2) Preparation of arsanic acid organic arsenic feed additive standard sample solution;

[0028] Weigh 28.98 mg of arsanic acid standard sample and 0.05 g of sodium hydroxide, dissolve them in deionized water, and finally make up to volume in a 100 mL volumetric flask. ready for dilution

[0029] (3) Collect actual water samples and extract possible organic arsenic feed additive compounds;

[0030] Take 5.0ml of the actual water sample and filter it through a 0.45μm filter membrane. Finally, place 0.01g of nano-ferromagnetic adsorbent in the corresponding actual water sample, adjust its pH value to 4.5 (using acetic acid...

example 2

[0041] (1) Tetrahymena medium preparation;

[0042] Weigh 10g of peptone, 2g of glucose, and 1g of yeast extract powder, dissolve them in 1000mL of deionized water, stir well, distribute them in 150mL Erlenmeyer flasks (80mL of medium in each bottle), and sterilize them in an autoclave at 121°C After 20-30min, let stand to room temperature, and store in refrigerator at 4°C.

[0043] (2) Preparation of arsanic acid organic arsenic feed additive standard sample solution;

[0044] Weigh 28.98 mg of arsanic acid standard sample and 0.05 g of sodium hydroxide, dissolve in deionized water, and finally make up to volume in a 100 mL volumetric flask. The total arsenic concentration in the corresponding standard solution is 100 μg / ml, and store in a refrigerator for future use Use diluted.

[0045] (3) Collect actual water samples and extract possible organic arsenic feed additive compounds;

[0046] Take 5.0ml of the actual water sample and filter it through a 0.45μm filter membran...

example 3

[0057] (1) Tetrahymena medium preparation;

[0058] Weigh 10g of peptone and 2g of glucose, dissolve them in 1000mL of deionized water, stir evenly, distribute them in 150mL Erlenmeyer flasks (each bottle has 80mL medium), sterilize in an autoclave at 121°C for 20-30min, and then statically Bring to room temperature and store in a refrigerator at 4°C.

[0059] (2) Roxars arsenic organic arsenic feed additive standard sample solution preparation;

[0060] Weigh 35.82mg of roxarsarsenic standard sample, 0.05g of sodium hydroxide, dissolve in deionized water, and finally make up to volume in a 100mL volumetric flask. Prepare for dilution.

[0061] (3) Collect actual water samples and extract possible organic arsenic feed additive compounds;

[0062] Take 5.0ml of the actual water sample and filter it through a 0.45μm filter membrane. Finally, place 0.01g of nano-ferromagnetic adsorbent in the corresponding actual water sample, adjust its pH value to 4.5 (using acetic acid-sod...

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Abstract

The invention relates to an organic arsenic feed additive discharge pre-alarming monitoring method in a water environment system, belonging to the environmental monitoring technical field, comprising the following steps: preparing tetrahymena culture medium; preparing arsanilic acid standard sample solution / roxarsone standard sample solution; collecting the actual water sample and separating and extracting the organic arsenic feed additive compound; inoculating the tetrahymena into the prepared culture medium and then subpackaged the inoculated culture medium into the sample culture tubes; respectively adding the organic arsenic feed additive standard solution into the sample culture tubes and respectively measuring the corresponding tetrahymena cell density using absorptometry; making the actual water sample intervening the corresponding tetrahymena biological model and analyzing and determining the corresponding tetrahymena cell density; based on the comparison of the tetrahymena cell density and the contrast of the corresponding tetrahymena cell density after the actual water sample intervention, to obtain the concentration range of the pollutant presence, thereby pre-alarming the discharge condition of the organic arsenic feed additive in the corresponding actual water environment.

Description

technical field [0001] The invention relates to a method in the technical field of environmental monitoring. Specifically, it relates to a method for early warning and monitoring of organic arsenic feed additive discharge in a water environment system. Background technique [0002] At present, p-arsanilic acid (p-arsanilic acid, arsanic acid) and 3-nitro-4-hydroxy-phenylarsine (roxarsone, roxarsone) are widely used as two main organic arsine feed additives in the world. Application, it has the functions of promoting meat growth, improving skin pigmentation, inhibiting coccidia eggs and mycoplasma, and resisting E. coli. It can be used in pigs and chickens to increase meat and egg production rates, thereby achieving the purpose of improving feed efficiency. . In 1964, the U.S. Food and Drug Administration (FDA) allowed Roxarsone to be used in the production of chicken feed, and in 1983 it was officially approved as a growth-promoting agent for pigs and chickens. In the 198...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N1/28
Inventor 韩晶晶孙群邹明强徐芳
Owner SHANGHAI JIAOTONG UNIV
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