375 results about "Calcium hydroxylapatite" patented technology

A mediator molecule is immobilized on the surface of a metallic or ceramic implant material. An anchor molecule such as a dialdehyde having a functional group that covalently binds the mediator molecule is covalently bound to the surface, and the mediator molecule is coupled to the functional group of the anchor molecule. The implant material may be composed of titanium, titanium alloy, aluminum, stainless steel or hydroxylapatite. Oxide units on the surface of the implant material can be increased preferably by treating with hot chromic-sulphuric acid for 0.5 to 3 hours at a temperature between 100 to 250° C. prior to binding the anchor molecule. Also, prior to binding the anchor molecule, the surface of the implant material can be activated by reacting with a silane derivative. Mediator molecules include BMP protein, ubiquitin and antibiotics, and the implant material may be an artificial joint or coronary vessel support such as a stent.

The invention discloses a method for removing heavy metal ions in sewage by nanometer hydroxylapatite, belonging to the technical field of heavy metal sewage treatment. The method comprises the following steps: adding nanometer grade hydroxylapatite powder in waste water containing heavy metal ions, wherein, the dosage of the hydroxylapatite powder is 6-8g / L; balancing the mixture for 12-48 hours at a temperature of 23-27 DEG C; and standing the mixture to remove the heavy metal ions in the waste water. The method uses the hydroxylapatite with relatively lower economic value to treat the heavy metal sewage, has high treatment efficiency, low treatment cost, strong field operability and small environment risk, and has heavy metal ion removal rate over 90 percent under conditions that concentration of Cd, Pb or Cu ions does not exceed 60mg / L.

The invention belongs to the field of a biomaterial, in particular to a preparation method for hydroxyapatite / polylactic acid composite biological coat on magnesium alloy surface, and solves the problem that an electrolytic deposition hydroxyapatite (HA) coat is not quite compact through sealing treatment of polylactic acid, so that the composite coat can improve corrosion resistance of magnesiumalloy in human body fluid remarkably, and biological degradability of the composite coat is used for adjusting service cycle of the magnesium alloy in vivo. The preparation method is as follows: the magnesium alloy is subjected to electrolytic deposition in water solution consisting of calcium salt, dihydrogen phosphate or hydrogen phosphate or other additives, so as to obtain a mixture coat of several calcium phosphates; the mixture coat is treated by alkali metal hydroxide solution and then transformed into an even HA coat; the HA coat is soaked in polylactic acid solution and subjected to the sealing treatment; and finally the HA coat is dried to obtain the hydroxyapatite / polylactic acid composite coat. The preparation method which combines biological activity of the HA and biological degradability of the polylactic acid organically is used for corrosion protection of the magnesium alloy in biotic environment.

The invention relates to a hydroxyapatite coating magnesium alloy medical implantation material and a preparation method thereof. The hydroxyapatite coating magnesium alloy medical implantation material is characterized in that: the surface of a magnesium alloy substrate is attached with a hydroxyapatite coating layer. The preparation method of the hydroxyapatite coating magnesium alloy medical implant material can adopt the bionic solution growing method, the ion beam deposition method, the coating and sintering process, the plasma spray method, the discharge plasma sintering method or the electrophoresis deposition method. The magnesium alloy material with the hydroxyapatite coating structure which is provided by the invention can effectively slow down the degradation rate of the magnesium alloy; at the same time, the coating layer can not only have great tissue compatibility, but can also be conductive to connective tissue attachment and the growth of the bone tissues, improve the bone healing rate and shorten the healing time.

Provided herein are methods for purifying recombinant A Disintegrin-like and Metallopeptidase with Thrombospondin Type 1 Motif 13 (ADAMTS13) protein from a sample. The method comprises enriching for ADAMTS13 protein by chromatographically contacting the sample with hydroxyapatite under conditions that allow ADAMTS13 protein to appear in the eluate or supernatant from the hydroxylapatite. The methods may further comprise tandem chromatography with a mixed mode cation exchange / hydrophobic interaction resin that binds ADAMTS13 protein. Additional optional steps involve ultrafiltration / diafiltration, anion exchange chromatography, cation exchange chromatography, and viral inactivation. Also provided herein are methods for inactivating virus contaminants in protein samples, where the protein is immobilized on a support. Also provided herein are compositions of ADAMTS13 prepared according to said methods.

The present invention relates to a process for preparing hydroxylapatite bio-ceramic membranes at titanium or titanium alloy surfaces by utilizing differential arc oxidation process. Firstly, preparing electrolytic solution A by using calcium ion salts and phosphate radical ion-containing salts, or preparing electrolytic solution B by adding metal silver ions to the electrolytic solution A; or preparing electrolytic solution C by adding metal silicon ions to the electrolytic solution A; and then, selecting titanium or titanium alloy as anodes and putting them respectively into the electrolytic solution A, B and C, selecting the non-corrodible steel container for containing the electrolytic solutions as cathodes, controlling conditions such as impulse electrical source positive phase voltage, frequency for differential arc oxidation of titanium or titanium alloy, then hydroxylapatite bio-ceramic membrane layers possessing different performances are prepared. Said titanium based bio-ceramic compound material prepared according to said process possesses not only intensity and toughness of metals but also biological activity of hydroxylapatites, and is capable of being applied in the fields such as jackstraw chirurgery implantation body and tooth planting body.

The invention relates to a medical polyurethane complex film and the preparation method thereof. The complex film is composed of fatty group polyurethane and nanometer hydroxylapatite, wherein the content of the anometer hydroxylapatite is 20-50 wt percent. The fatty group polyurethane is polymerized by at least one of fatty group vulcabond and polyether or polyester polyol, wherein the mol ratio of the fatty group vulcabond-NCO and -OH in the polyol is 1.5-3 to 1. The film belongs to a thermoplasticity elastic film, has favorable elasticity and easy moulding, can load microspheres containing medicine or active components such as biological nutrilit and the like and structures distributed with through holes, thereby having favorable biological performance, and the film does not contain aromatic components but contains ester groups and / or hydrophilic ether groups which can be hydrolyzed or biologically degraded; the hydrophile and hydrophobic nature balance and the degradation of the film can be changed through adjusting the proportion, and the film can be applied to the medical health field of bone tissue guiding regeneration film and medicine slow-released film, and the like.

The invention relates to a calcium silicate / hydroxylapatite composite biological ceramic material and a preparation method and application thereof, belonging to the field of biological materials. Calcium silicate / hydroxylapatite nano composite powder prepared by a chemical method is used as a raw material and carried out the dry pressing and molding to obtain a material biscuit, and the biscuit is calcined at 1000-1200 DEG C for 1-10 hours to obtain a degradable calcium silicate / hydroxylapatite composite biological ceramic material which has good mechanical properties and is used for repairing the defects of hard tissues. The biological activity, the degradability and the mechanical strength of the biological ceramic material of the invention can be adjusted and controlled. The invention has simple and easy processes and convenient popularization.

Aiming at the problems of low strength and low toughness of bioactive ceramics, the invention provides a method for strengthening and toughening a bioactive ceramic material by use of graphene and preparing graphene / biological ceramic artificial bone by a selective laser sintering (SLS) technology. The method has the advantages that graphene is added into the bioactive ceramic (hydroxylapatite, calcium phosphate, bioglass, calcium silicate and the like) as a nano enhanced phase, and the strength and toughness of the ceramic can be remarkably improved by use of the excellent mechanical property of graphene; the SLS technology is applied to the preparation process of graphene / biological ceramic artificial bone, and the sintering time can be shortened to the second level and even millisecond level by use of the characteristic of quick heating and cooling of laser so as to avoid structural injury of graphene caused by long-time high-temperature effect. The method for strengthening and toughening the ceramic material by use of graphene provided by the invention is of great significance to the application of bioactive ceramic to the load bearing parts of the human body.

The invention discloses a method for preparing hydroxylapatite / chitosan composite coat by electrochemical deposition. The method comprises the following steps: preparing electrolytic solution with certain concentration and pH value from calcium salt, phosphate and chitosan; carrying out electrodeposition in an electrolysis bath with a three-electrode system; using a saturated calomel electrode as a reference electrode, a platinum plate as a counter electrode, and biomedical metal to be coated as a working electrode; depositing compositions in the coat on a base material in ion mode for 25 minutes, with minus 1.2 volts of high pulsing electric potential, minus 2.0 volts of low pulsing electric potential, 100s of pulse width, and evenly dispersing the chitosan in a composite coat, so as to finally produce the evenly hybridized hydroxylapatite / chitosan composite coat.

The invention relates to a method for purifying at least one enzyme obtained in an excess fermentation of Clostridium histolyticum. It is provided for that the enzymes of the excess fermentation are separated by a multistage chromatography method by exclusively using chromatography materials on styrene / divinyl-benzene base and / or on base of in particular ceramic hydroxylapatite.

The invention discloses a method for preparing a hydroxyapatite-natural macromolecular nano compound with a one-pot method. The method disclosed by the invention has the core characteristics that raw material powder is directly added, and mainly comprises the following steps: adding calcium salt and phosphate into a natural macromolecular solution according to the amount of substance ratio of Ca / P of 5 to 3; adjusting the pH of the system to 10 to 12 by using an alkali solution, wherein calcium ions, phosphate ions and hydroxyl ions in the system and natural macromolecules have an in-situ co-precipitation reaction; washing a reaction product to be neutral, and drying to obtain the hydroxyapatite-natural macromolecular nano compound; grinding and sieving to prepare nano composite particle products with different specifications. The method disclosed by the invention is simple and has moderate reaction conditions and small energy consumption; the obtained hydroxyapatite-natural macromolecular nano compound has good granularity, rigidity, microscopic appearance and crystallinity, and can be widely applied to the fields of bone defect repairing tissue engineering materials and medical equipment and the like of dental department, orthopedic surgery and the like.

The invention relates to the field of surface modification of biomedical materials, in particular to a biological active coating on the surface of titanium or a titanium alloy and a preparation method thereof. The coating is a composite gradient coating; the inner layer is a titanium oxide film layer with the thickness between 0.5 and 10 mu m; the intermediate layer is a sodium titanate gel film layer with the thickness between 0.1 and 10 mu m; and the outer layer is a hydroxylapatite layer with the thickness between 1 and 15 mu m. A method combining anodization and alkali treatment is adopted to prepare the biological active coating on the surface of the titanium or the titanium alloy, which comprises the following steps: firstly, preparing an oxide film with certain thickness on the surface of the titanium or the titanium alloy by an anodization method; secondly, forming a layer of porous netty sodium titanate gel film layer on the surface of the oxide film by an alkali treatment method; and finally, depositing the bone-like hydroxylapatite layer on the surface of a sample after the treatments in a simulated body fluid. The method can effectively improve the biological activity on the surface of the titanium or the titanium alloy; and the surface has a coarse porous structure, so the bonding force of the hydroxylapatite layer and the adhesive ability of cells are improved.

The invention discloses a preparation method of porous microspheres composite with hydroxyapatite microspheres being capable of carrying drugs and bone cement, which belongs to the technical field of biological material preparation. The method comprises the following steps: the preparation of nano hydroxyapatite powders; the preparation of Alpha- tricalcium phosphate powders; the preparation of the hydroxyapatite microspheres; and the preparation of the porous microspheres composite with the hydroxyapatite microspheres and the bone cement. The method has the advantages that: the porosity of the porous microspheres composite with the hydroxyapatite microspheres and the bone cement, and the size of the microspheres can be easily controlled; the composite of the drugs can be carried out by the preparation process of the bone cement stuff, the drug-carrying rate is large and controllable; and the composite porous microspheres can be served as drug carriers or stents with integration of bone restoration and cure.

The present invention relates to a kind of composite hydroxyapatite / carbon nanotube material and its preparation process. The composite material consists of mainly two components including hydroxyapatite and carbon nanotube. The technological process includes chemical preciptiation to synthesize hydroxyapatite, and compounding directly with carbon nanotube to prepare compoiste hydroxapatite / carbon nanotube material. The composite material possesses excellent mechanical performance and biocompatibility and certain magnetism and wave absorbing performance, so that the composite material may be used in repairing and replacement of body bone and extracorporeal physical treatment of bone diseases, and may have latent application as body's bearing bone, magnetic and wave-absorbing material.

The invention provides a method for preparing a hydroxyapatite / titanium dioxide composite bioactive coating on the surfaces of medical metals such as a titanium alloy, and the like, and belongs to the technical field of the preparation of bioactive coatings on the surface of the titanium alloy. The composite coating is prepared by preparing precursor sols of HA and TiO2 respectively, then mixing the precursor sols of the HA and the TiO2, and performing aging, coating, drying and heat treatment. The coating has high bioactivity, low cost of preparation technology, and high bonding strength with a matrix, is applicable to the preparation of surface coatings of various complicated geometric structures, and can be used for surface modification of surgical implants such as artificial hip joints and dental implants as well as orthopedic instrument products.

An absorbable internal fixing material for bone fracture is prepared from polylactic acid (50-90 wt.%), tricalcium phosphate (1-40 wt.%) and hydroxylapatitie (1-40 wt.%) through mixing then and then hot die pressing at 80-1230 deg.C and under 0.11-25 MPa or hot extruding at 80-230 deg.c. The granulality of hydroxylapatite and tricalcium phosphate is less than 100 microns. Its advantages are high strength, high strength, high X-ray visualization and high biocompatibility.

The invention discloses a microwave-assistant preparation method of hydroxylapatite nanometer structure porous microspheres. The microwave-assistant preparation method comprises the following steps of: mixing water-soluble calcium salt as a calcium source with pyridoxal phosphate as a phosphor source under an acidic condition; preparing hydroxylapatite nanometer structure porous microspheres as a carrier through a microwave-assistant hydrothermal reaction; adding the hydroxylapatite nanometer structure porous microspheres to organic solvent containing a hydrophobic drug or an aqueous solution containing hydrophilic protein; and oscillating the solution for 24-60 hours at 20-40 DEG C, and centrifuging, washing and drying the products to obtain the hydroxylapatite nanometer structure porous microspheres which are loaded with the hydrophobic drug or adsorbed with the hydrophilic protein.

Provided is a method for production of nanocrystalline hydroxyapatite particles, and nanocrystalline hydroxyapatite particles produced according to the method. The nanocrystalline hydroxyapatite particles exhibit substantially superior cell transformation abilities as compared to known and commercially-available calcium phosphate kits. The nanocrystalline hydroxyapatite particles also find use in tissue engineering applications, for example bone and tooth engineering and repair applications.

The present invention is one kind of nanometer hollow spherical granular hydroxyapatite and its preparation process. The nanometer hollow spherical granular hydroxyapatite has diameter of 130-170 nm, wall thickness of 40-50 nm, crystalline hydroxyapatite content of 40-60 % and amorphous calcium phosphate for the rest. The preparation process includes the following steps: dropping calcium salt solution slowly into solution containing phosphate radical and CTAB as template agent at certain temperature and in ultrasonic condition, dropping ammonia water solution to regulating the solution pH value to 9-10 and obtain milk white suspension, filtering, washing and low temperature vacuum drying. The nanometer hollow spherical granular hydroxyapatite has simple preparation process, light weight, great specific surface area and important application foreground in medicine release controlling carrier, medicated tissue engineering rack, etc.

The invention discloses a method for preparing chitosan and hydroxyapatite composite bone support materials, which comprises the following steps: a precursor solvent of hydroxyapatite is prepared, and mixed solvents of chitosan and hydroxyapatite are prepared and placed still for defoaming; mixed solvents are evenly applied on the surface of a mould, and placed in alkalescence concreting liquid at room temperature to be concreted into a membrane; then the mixed solvents are injected into the mould, and the mould is put into the alkalescence concreting liquid again; after concretion and demoulding, gel compounded by the chitosan and the hydroxyapatite is obtained, and distilled water is used for washing the gel to neutrality; and only predry, re-freezing and desiccation in vacuum are needed. The support materials have the advantages of three-dimension sequence, good mechanical properties and high porosity, thus promoting the absorption and the growth of cells. The hydroxyapatite granules are evenly distributed in the chitosan kinetosome and can keep the continuous, stable and adagio release of calcium ions and phosphor ions in hydroxyapatite from chitosan kinetosome during the bone repairing process, thus avoiding burst release.

The invention relates to a preparation method of a hydroxylapatite / sodium alginate nanometer composite material, which mixes calcium hydroxide and phosphoric acid water solution according to the chemical stoichiometric ratio of Ca / P in the hydroxylapatite and carries out the in-situ composite reaction with sodium alginate water gel, and a three dimensional net shaped structure similar to a natural bone is formed by adding calcium chloride to realize the intercrossing reaction with the sodium alginate. The method has the advantages that: the hydroxylapatite / sodium alginate nanometer composite material maintains the good biological compatibility, biological activity and bone conductibility of HA; the combination of the sodium alginate as an organic substrate with the HA can bestow the HA good processing and forming property, thus effectively inhabiting the moving of HA particles from the transplanting parts; and the sodium alginate has rich source and low price, and the preparation process can be operated at normal temperature and pressure, and has simple operation and low cost, and is an ideal bone repairing substitute materials of the new generation.

The invention relates to an artificial bone with a porous laminated structure and passages, which belongs to the technical field of biomedical materials. The artificial bone is alternately laminated and formed by compact layers and porous layers which are made of calcium phosphate base biological ceramic materials, and in addition, passages for conveying cells and body fluid are arranged in the direction which forms a set angle with the laminated layers. The preparation method comprises the following step: mixing powder such as hydroxylapatite, beta-tricalcium phosphate and the like through using deionized water as medium to be prepared into pulp. A three-dimensional gel laminated forming system is adopted for preparing ceramic blanks formed by the compact layers, the porous layers and the passages, and then the ceramic blanks are sintered at a high temperature for preparing the artificial bone. The invention has the characteristics that the artificial bone has high mechanical strength, has the porous structure with the effects of osteoplast adhesion, propagation, growth and vascularization, can be used as the artificial bone, can also be used as a bone tissue engineering support frame, and has wide application prospects in clinics in the orthopedics department.

The present invention is directed to magnesium phosphate ceramics and their methods of manufacture. The composition of the invention is produced by combining a mixture of a substantially dry powder component with a liquid component. The substantially dry powder component comprises a sparsely soluble oxide powder, an alkali metal phosphate powder, a sparsely soluble silicate powder, with the balance of the substantially dry powder component comprising at least one powder selected from the group consisting of bioactive powders, biocompatible powders, fluorescent powders, fluoride releasing powders, and radiopaque powders. The liquid component comprises a pH modifying agent, a monovalent alkali metal phosphate in aqueous solution, the balance of the liquid component being water. The use of calcined magnesium oxide as the oxide powder and hydroxylapatite as the bioactive powder produces a self-setting ceramic that is particularly suited for use in dental and orthopedic applications.

Disclosed herein is a dental material composition comprising a polymer matrix material and a biocatalyst that promotes the deposition of a mineral, for example silica or hydroxylapatite. A dental restoration comprising the polymer matrix material and biocatalyst is also claimed. In another embodiment, a method of forming a dental material comprises combining a biocatalyst that promotes the deposition of a mineral and a polymer matrix material to form a biocatalyst-polymer composite.

The invention relates to an integral bionic cervical disc prosthesis, and belongs to the field of medicalimplant materials. The cervical disc prosthesis consists of an upper rigid terminal plate, a lower rigid terminal plate, an elastic body nucleus pulposus material and an outer layer protection film material, wherein the elastic body nucleus pulposus material is used for connecting the upper and lower rigid terminal plates; the elastic body material and the upper and lower rigid terminal plates are integrally formed in situ; through the structure arrangement of rigid terminal plate cavities, grooves, bulges and the like, the firm combination of the nucleus pulposus material and the rigid terminal plates is realized; a mutually embedding and covering integral structure is formed; and potential danger of material damage and abrasive dust caused by interface friction of the rigid terminal plates and the nucleus pulposus material is effectively reduced or eliminated. The surfaces, in contact with the bone of the human body, of upper and lower rigid terminal plates adopt the arc-shaped design; titanium or hydroxylapatite coatings cover the surfaces; spurs or sawtooth-shaped fixed teeth are distributed on the surface; and the immediate post-surgical stability and the bone integration are improved. The cervical disc prosthesis can realize the rotating, bending-stretching and transverse moving three-dimensional six-freedom-degree space movement; and the normal intervertebral disc moving functions in the human body can be met.

The invention discloses a hydroxylapatite / biodegradable polyester composite material and a preparation method thereof. The preparation method includes the following steps: under conditions of no water, no oxygen, and argon protection, the composite material is obtained by carrying out an in-situ polymerization reaction of hydroxyapatite and an aliphatic cyclic monomer under catalysis of stannous octoate; the aliphatic cyclic monomer is selected form at least one of lactide, epsilon-caprolactone and glycolide; and the composite material provided by the invention comprises hydroxylapatite and a biodegradable polyester. The composite material provided by the invention has the surface enriched with a hydroxylapatite layer having a biological activity, and has an excellent biological compatibility and the biological activity; an biological activity interface can rapidly induce calcium ion deposition in a physiological environment so as to induce nucleation and growth of apatite, and imitates inorganic / organic components of a natural bone matrix in composition; based on the above characteristics, the modified hydroxylapatite / biodegradable polyester composite material is a good support material for repairing bone defects, and has good application prospects in the fields of cell expansion and bone tissue engineering.

The invention discloses a method for preparing hydroxyapatite nano-structure microspheres, which comprises the following steps of: mixing a calcium source and a phosphorus source in a molar ratio of n(Ca2+) to n(PO43-) of 5:3; adding water into the mixture and regulating the pH of a system to the range of 0.5 to 3.5 by acid; then adding a chelating agent and urea; adding glycerine; and preparing the hydroxyapatite nano-structure microspheres by a solvent thermal synthesis method, wherein the volume ratio of glycerine to water is 0-12:1 and the concentration of the calcium source is in the range of 0.02 to 0.2mol / L. The method adopts a one-step synthesis mode. A surfactant is not used. The method has simple preparation process and relatively mild reaction condition. The hydroxyapatite nano-structure microspheres have good biocompatibility and large specific surface area and have loading rate and obvious release effect when being used as a medicament release carrier.

The invention relates to a preparation method of nano hydroxylapatite doped with metal ions, which comprises the following steps of: firstly, respectively preparing Ca(NO3)2 and (NH4)2HPO4 solution, mixing the Ca(NO3)2 and (NH4)2HPO4 solution, and obtaining clear and transparent solution A; adding metal nitrate into solution A and obtaining clear and transparent solution B; adding CO(NH2)2 into solution B, and obtaining solution C; finally heating and backflowing the solution C for 3 to 5 hours with the temperature of 100 DEG C under the magnetic force stirring; after the reaction is end, naturally cooling to room temperature; then vacuum-filtering, cleaning with water, drying for 24 hours under the temperature of 100 DEG C; and finally, obtaining nano hydroxylapatite crystal doped with metal ions. The preparation method adopts a metal salt solution heating even precipitation method, and takes inorganic salts containing calcium and phosphate group as raw materials; urea is acidity of a control reaction system of an additive; metal salts are added according to the doped proportion of 1 percent to 5 percent; reaction is carried out under the magnetic force stirring and heating conditions; and the backflowing of reaction liquid is adopted to synthesize nanoscale metal-doped nano hydroxylapatite crystal by one step. The method is obviously characterized by even doping and easy control for added amount.