The invention provides a
seedling strengthening and rapid propagation method for
polygonatum cyrtonema, which comprises the following steps: (1)
cutting polygonatum cyrtonema cluster buds into blocks, inoculating a proliferation,
seedling strengthening and propagation medium with the blocks for subculture, and carrying out repeated
cutting and subculture for multiple times to obtain robust cluster buds; (2)
cutting the robust cluster buds into
bud tubers with adventitious buds, and inoculating a rooting culture medium with the
bud tubers for rooting culture; (3) putting rooted test-tube plantlets in a culture room for hardening under light, taking out the rooted test-tube plantlets from a culture
bottle, and washing off the culture medium at the roots of the rooted test-tube plantlets; then, soaking the rooted test-tube plantlets with
carbendazim, and after soaking is completed, planting the rooted test-tube plantlets into matrix mesh bags, and watering the rooted test-tube plantlets sufficiently with root fixing water; and finally, culturing the rooted test-tube plantlets in a
greenhouse. According to the method, the
polygonatum cyrtonema buds are subjected to induced differentiation to obtain the cluster buds, the operation is simple, the culture cost is low, the propagation efficiency is high,
tissue culture industrialized production can be achieved, and the method is an important way for excellent
germplasm preservation and
seedling batch production of
polygonatum cyrtonema.