The invention provides an industrial cultivation method of selenium-enriched agaricus bisporus, The cultivation materials comprises the materials based on the following part by weight: 100-300 parts of straw, 100-300 parts of cow dung, 0.5-1 part of urea, 0.5-1 part of ammonium bicarbonate, 0.5-1 part of superphosphate, 1-2 parts of lime, 1-2 parts of cake fertilizer, 0.1-0.3 parts of sodium selenite, etc. The invention also provides a series of industrial cultivation methods such as the preparation of mushroom seeds, the preparation of paved soil, the cultivation of the selenium-enriched agaricus bisporus, etc. The cultivation method has low cost, simple and convenient operation and easy realization, and can cultivate the agaricus bisporus with high yield and abundant nutrition, wherein the content of organic selenium generally can reach more than 0.5-1%. The selenium-enriched agaricus bisporus is dried in a drying oven till that moisture content is less than 5%, is crashed and screened to obtain the organic selenium powder; and the organic selenium powder is taken as raw materials to be matched with spirulina, lucid ganoderma, and the like, to prepare various selenium-enriched nutritious foods such as granules, troche, oral liquid, and the like, thereby having wide application range.