The invention discloses a single-pump droplet generation
system, the single-pump droplet generation
system comprises a single pump, a water phase
injector connected with the output end of the single pump and used for injecting a water phase, a flow guide
pipe communicated with an injection head of the water phase
injector, an adjusting mechanism used for clamping the flow guide
pipe and adjustingthe height of the
tail end of the flow guide
pipe, a liquid collecting
bottle and a centrifugal pipe arranged in the liquid collecting
bottle and used for containing an
oil phase. And the
tail end ofthe flow guide pipe extends into the centrifugal pipe, and a preset distance for generating liquid drops with preset sizes is kept between the
tail end of the flow guide pipe and the bottom end of thecentrifugal pipe. Thus, the single pump is used as a power source to drive the water phase
injector to inject the water phase into the flow guide pipe, and then the
oil phase is contained in the centrifugal pipe, so that the injected water phase and the
oil phase are mixed to generate droplets required by a reaction, and on the basis of smoothly generating digital PCR droplets, the space occupation of equipment and the
system structure complexity can be reduced; meanwhile, the research cost is reduced. The invention also discloses a digital PCR device, which has the beneficial effects as described above.