The invention discloses a
pleurotus nebrodensis liquid strain culture medium, comprising the following raw materials in parts by weight: 4-4.5 parts of
bran, 2-2.5 parts of
corn flour, 1.2-1.6 parts of soybean cake
powder, 1.5-2 parts of glucose, 0.1-0.12 part of peptone, 0.1-0.12 part of KH2PO4, 0.05-0.06 part of MgSO4 and 0.01 part of VB1 in 100 parts of water. A propagation method of a
pleurotus nebrodensis strain comprises the following steps: preparing a
liquid culture medium; adjusting the pH value of the
liquid culture medium; sterilizing, and inoculating
solid strain seeds; cultivating and preparing a
solid fungus bag medium; cultivating
solid strains; and inoculating and cultivating a fruiting
fungus bag. The
liquid culture medium disclosed by the invention is abundant in
raw material source, and low in price; and due to a solid-liquid-solid production mode, compared with a traditional complete solid culture method, hyphae are even in
growth speed in the
fungus bag, regular and consistent in fruiting, even in sporocarp size, good in
mushroom shape, good in quality, short in
fermentation cycle, high in yield, and high in
economic benefits, and thus the method is very suitable for popularization in small and medium-sized enterprises and peasant households.