49 results about "Yolk Proteins" patented technology

The invention discloses a zebra fish vitellogenin indirect competition ELISA kit and a detection method and application of the kit. The method comprises the following steps: respectively purifying lipovitellin and vitellogenin from zebra fish ovary and 17beta-estradiol exposed zebra fish body by using a method for combining two-step chromatography and ultrafiltration, and preparing rabbit anti-zebra fish vitellogenin polyclonal antibody; respectively filling a pure zebra fish lipovitellin product, the rabbit anti-zebra fish vitellogenin polyclonal antibody, a blank 96-pore ELISA plate, a coating solution, a washing solution, a blocking solution, sample diluent, a color developing solution, a stopping solution and a horse radish peroxidase labeled goat anti-rabbit second antibody into a box body, thereby obtaining the indirect competition ELISA kit for detecting the zebra fish vitellogenin. The kit disclosed by the invention can be applied to investigation of environmental estrogen substances, the vitellogenin in zebra fish plasma, overall homogenate, liver tissues and hepatic cell culture solution can be sensitively, accurately, conveniently and quantitatively detected, the detection range is 31.25-1000ng mL<-1>, and an important tool is provided for the application of the zebra fish in the field of endocrine disrupter detection.

The present invention relates to the gene, protein, polyclonal antibody and application of the vitellogenin of the spiny sea plate, and belongs to the field of molecular biology. The gene sequence of the spinous sea plate vitellogenin is SEQ ID NO: 1, and the protein is as follows: Described in SEQ ID NO:2. Utilize the ELISA method of the Vg antibody of the spiny carp Vg, by identifying the Vg content in the organism, and then warn the pollution status of EEs in the marine ecosystem, the detection kit prepared by using the spiny spiny Vg antibody can detect a certain The degree of estrogen pollution in the sea area is accurate and reliable.

The method for interspecific cross-breeding of charred trout with the male parent of charred trout belongs to the technical field of aquaculture. Broodstock breeding is carried out under different water temperature conditions, so that two fishes with different reproductive periods mature synchronously, hold eggs through isotonic fluid, fully stir to dissolve the yolk protein of broken eggs, carry eggs for insemination, and hatch fertilized eggs by holding eggs in a bucket and basket , FRP parallel tank hatch eye eggs. In particular, the cultivation of the male red salmon at low temperature not only prolongs the maturation time, so that it matures synchronously with the white salmon or American char, but also, at low temperature, the semen quality is good, the duration is long, and the breeding time is long. Interbreeding effect is good. By the method of the present invention, the biological traits of part of the red salmon are obtained, the muscle quality is similar to that of the red salmon, the growth rate is 50% to 60% faster than that of the red salmon, and the disease resistance is strong, which improves the breeding efficiency and benefit.

The invention provides an application and a method utilizing a bombyx mori linnaeus vitellogenin promoter for gender-specific and tissue-specific expression of an exogenous gene. The method breaks through a limitation that a traditional transgenic bombyx mori linnaeus cannot effectively perform male-specific, female-specific and tissue-specific expression of the exogenous gene, and develops and utilizes the bombyx mori linnaeus vitellogenin promoter for gender-specific and tissue-specific expression of the exogenous gene in bombyx mori linnaeus cell lines and the transgenic bombyx mori linnaeus for the first time. Therefore, with the first time of utilization of gender expression controllability and tissue expression controllability of the transgenic bombyx mori linnaeus, the application field of the bombyx mori linnaeus is expanded, the sustainable development of sericulture is maintained, and genetic control of insect pests can be studied.

The invention discloses polypeptides and nucleic acids that mediate the endocytosis of exogenous proteins and their application, and a method for gene editing in Neocaridina heteropoda. The invention finds a length of A polypeptide of only 40aa, the polypeptide has an unexpected effect of mediating the endocytosis of exogenous proteins, so it is easy to express, purify, form RNP complexes and be endocytosed, and has a good effect in the gene editing of Neopodia prawn Application prospects. This technology can be widely applied to non-model organisms that are not yet proficient in embryonic microinjection technology, and is of great significance for Cas9-mediated gene editing.

The invention discloses a method for preparing main yolk protein from sea cucumber body cavity fluid. The specific process includes: pretreatment of sea cucumber body cavity fluid, low-temperature desalination by dialysis, extraction of main yolk protein, reconstitution, dialysis and drying. The present invention uses sea cucumber coelom fluid, a by-product of the sea cucumber processing process, as a raw material, improves the utilization rate of the by-product in the sea cucumber processing process, makes its protein resources fully developed and utilized, and reduces the pollution of sea cucumber by-products to the environment at the same time. The development and utilization of the main vitellin in body cavity fluid is of great significance. Compared with traditional protein precipitation methods such as ammonium sulfate salting out, ethanol precipitation, isoelectric point precipitation and other methods that are not easy to produce on a large scale, the present invention has mild operating conditions and is less likely to cause denaturation of biological macromolecules. And the extraction rate is as high as 70%. The method of the invention is simple, and can process a large number of samples at one time without the assistance of complex instruments, and is suitable for large-scale production.

A bone-strengthening composition of the invention comprises as an active ingredient an egg-yolk protein hydrolysate in which a proportion of the area of the region of a molecular weight of 500 or more and 20,000 or less to the total proportion of the area of proteins, peptides and amino acids is 50% or higher in a molecular weight distribution analysis by gel filtration chromatography. The composition has an action of osteoblast growth promotion, bone growth and bone strengthening, is effective in the prevention and treatment of a variety of bone diseases such as osteoporosis, and is useful as a safe and inexpensive product that can be widely used for a food and drink, a pharmaceutical or a feed and can be taken daily and continuously. The egg-yolk protein hydrolysate is preferably a hydrolysate obtained by defatting egg yolk with an organic solvent and hydrolyzing the resulting powder with a protein hydrolyzing enzyme.