A method for preparing main vitellin from sea cucumber body cavity fluid

A technology of yolk protein and coelom fluid, which is applied in the preparation methods of peptides, chemical instruments and methods, peptide sources, etc., can solve the problems of waste of resources, environment, pollution, etc.

Active Publication Date: 2021-05-14
DALIAN POLYTECHNIC UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Sea cucumber body cavity fluid is a by-product of the sea cucumber processing process, which causes waste of resources and environmental pollution after being discarded as waste
Sea cucumber is rich in nutrition and has high economic value. Its body cavity fluid contains a variety of active ingredients, but it has not been well utilized. At present, the research on sea cucumber body cavity fluid is limited to the research on immune components. Sea cucumber body cavity fluid is also rich in The main yolk protein in the sea cucumber has high nutritional value and is rich in essential amino acids. If the main yolk protein in the coelom fluid of sea cucumbers can be recycled, it will be of great significance to the high-value utilization of sea cucumbers

Method used

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  • A method for preparing main vitellin from sea cucumber body cavity fluid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]S1, seatron body cavity pretreatment: Using filter paper to dry the liquid, the seafood is cut from the abdomen, collect the body cavity, centrifuge at 4 ° C, 1500 × g, collect the supernatant, to the body cavity Liquid;

[0035]S2, dialysis method low temperature removal salt: The phosphate buffer solution of 10 mM pH7 is dialyzed 48 h at 4 ° C for 4 ° C, and the amount of phosphate buffer is 10 times;

[0036]S3, extracting the main yoghuine: Under 4 ° C, the polyethylene glycol of the molecular weight 6000 is added to the seatrogan cavity liquid obtained by step S2, and the final concentration of the polyethylene glycol is 10% (weight), using 0.5 mol. / L NaOH solution or 0.5 mol / L of hydrochloric acid solution adjusted the reaction system pH of 7.0, standing for 3 h, 7500 g from centrifugation for 10 min, the resulting precipitate as the main yoglogin;

[0037]S4, reconstition: The primary peclancin precipitate obtained by step S3 is reconstituted with 10 mm pH 7.0 of a phosphate...

Embodiment 2

[0043]S1, seatrovic cavity pretreatment: Use filter paper to dry the liquid, cut the sea cucumber from the abdomen, collect the body cavity, centrifuge at 4 ° C, 2000 × g from 10 min, collect the supernatant, to the body cavity Liquid;

[0044]S2, dialysis method, low temperature removal salt: using a 3.5 kDa dialysis film, 20 mM pH 7.4 phosphate buffer solution of 20 mm pH 7 ° C for dialysis 60 h, and the amount of phosphate buffer is used as a body cavity. 15 times;

[0045]S3, extracting the main yoyangoprotein: Under 4 ° C, the polyethylene glycol of the molecular weight 6000 is added to the seatrogan cavity liquid obtained in step S2, so that the final concentration of the polyethylene glycol is 12% (weight), using 0.5 mol / L NaOH solution or 0.5 mol / L of hydrochloric acid solution adjusted the reaction system pH of 7.5, standing for 6 h, 8000 g from 20 min, and the resulting precipitate is the main yogglycin;

[0046]S4, reconstition: The primary peclancin precipitate obtained by st...

Embodiment 3

[0052]S1, seatron body cavity pretreatment: Use filter paper to dry the liquid, cut the sea cucumber from the abdomen, collect the body cavity, centrifuge at 4 ° C, 1800 × g conditions of 12 minutes, collect the supernatant, to the body cavity Liquid;

[0053]S2, dialysis method low-temperature salt: using 4 kDa dialysis film, 25 mM pH 8 phosphate buffer solution to dialysis 72 h, the amount of phosphate buffer is 20 times, control The temperature of the phosphate buffer solution temperature at 4 ° C

[0054]S3, extracting the main yoghuine: Under 4 ° C, add polyethylene glycol of molecular weight 6000 to the seatron cell cavity obtained in step S2, and the final concentration of polyethylene glycol is 14% by weight, 0.5 mol / L NaOH solution or 0.5 mol / L of hydrochloric acid solution adjusts the reaction system pH 8, stands for 9h, 8500 g from 15 min, the resulting precipitate is the main yoghuine protein

[0055]S4, reconstition: Pelletizing step S3 is reconstituted by 20 mL / g of a pho...

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Abstract

The invention discloses a method for preparing main yolk protein from sea cucumber body cavity fluid. The specific process includes: pretreatment of sea cucumber body cavity fluid, low-temperature desalination by dialysis, extraction of main yolk protein, reconstitution, dialysis and drying. The present invention uses sea cucumber coelom fluid, a by-product of the sea cucumber processing process, as a raw material, improves the utilization rate of the by-product in the sea cucumber processing process, makes its protein resources fully developed and utilized, and reduces the pollution of sea cucumber by-products to the environment at the same time. The development and utilization of the main vitellin in body cavity fluid is of great significance. Compared with traditional protein precipitation methods such as ammonium sulfate salting out, ethanol precipitation, isoelectric point precipitation and other methods that are not easy to produce on a large scale, the present invention has mild operating conditions and is less likely to cause denaturation of biological macromolecules. And the extraction rate is as high as 70%. The method of the invention is simple, and can process a large number of samples at one time without the assistance of complex instruments, and is suitable for large-scale production.

Description

Technical field[0001]The present invention belongs to the field of food processing, involving a preparation method of protein, and more particularly to a method of preparing a major yumberrin from a seatron cell chamber.Background technique[0002]The seahth cell cavity is by-product in the seatron processing process, which has caused resource waste and environmental pollution as waste. Haijing nutrients are rich in nutrients, high economic value, and have a variety of active ingredients in their body cavity, but are not well used. At present, the research on sea-categoric cavity is limited in the study of immune components, and the sea cucumber cavity is also rich The main yoglobin, the main egg yolk protein has a high nutritional value, rich in essential amino acids, if it can recover the main egguminum in the sea cell cavity, it will be important to the high - value utilization of sea cucumber.Inventive content[0003]SUMMARY OF THE INVENTION It is an object of the present invention ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C07K1/30C07K1/34C07K1/14
CPCC07K14/43504
Inventor 吴海涛李傲婷商文慧杜椅楠姜昕昱洪佳男
Owner DALIAN POLYTECHNIC UNIVERSITY
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