268 results about "Dialysis method" patented technology

Novel lipid-nucleic acid particulate complexes which are useful for in vitro or in vivo gene transfer are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. Upon removal of a solubilizing component (i.e., detergent or an organic solvent) the lipid-nucleic acid complexes form particles wherein the nucleic acid is serum-stable and is protected from degradation. The particles thus formed have access to extravascular sites and target cell populations and are suitable for the therapeutic delivery of nucleic acids.

Plasmid-lipid particles which are useful for transfection of cells in vitro or in vivo are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. The particles are typically 65-85 nm, fully encapsulate the plasmid and are serum-stable.

Novel lipid-nucleic acid particulate complexes which are useful for in vitro or in vivo gene transfer are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. Upon removal of a solubilizing component (i.e., detergent or an organic solvent) the lipid-nucleic acid complexes form particles wherein the nucleic acid is serum-stable and is protected from degradation. The particles thus formed have access to extravascular sites and target cell populations and are suitable for the therapeutic delivery of nucleic acids.

The invention relates to a process for simultaneously extracting pigments, saponins and polysaccharides of sapindus, and preparation and application of the saponins of the sapindus, and belongs to the technical field of medicine and chemical engineering. The process includes extracting total extracts of the sapindus from dry fruit peel of the sapindus by the aid of a decocting method, dissolving the total extracts in water to obtain samples, feeding the samples to macroporous adsorption resin, eluting the macroporous adsorption resin by distilled water at first, collecting eluent, removing proteins by the aid of a dialysis method, and concentrating and drying the eluent to obtain polysaccharide extracts of the sapindus; eluting the macroporous adsorption resin with 4-5 retention volumes by the aid of 40% ethanol, then eluting the macroporous adsorption resin by the aid of 80% ethanol, collecting eluent and concentrating and drying the eluent to obtain saponins extracts of the sapindus; eluting the macroporous adsorption resin by the aid of 95% ethanol, collecting eluent and concentrating and drying the eluent to obtain pigment extracts of the sapindus. Active dry yeast is inoculated in the saponins extracts of the sapindus after the saponins extracts of the sapindus are dissolved in water, then the saponins extracts of the sapindus are fermented and are concentrated and dried to obtain the saponins of the sapindus. The process, the preparation and the application have the advantages of simplicity in preparation method, low cost, one-step reaction, environmental protection, high efficiency and easiness in popularization and application.

A hemostatic agent designed for use in cases of non-compressible hemorrhage. It can be applied through a mixing needle and / or a spray injection method following abdominal, chest, extremities or other intracavitary severe trauma to promote hemostasis, or it can be used for laparoscopic procedures or other surgical procedures in which compression is not possible or recommended. Its crosslinking technology generates an adhesive three-dimensional polymeric network or scaffold that carries a fibrin sealant required for hemostasis. When mixed, it produces a foam that spreads throughout a body cavity reaching the lacerated tissue to seal tissue and promote the coagulation cascade. The fibrin components are produced by a novel dialysis method which does not present thrombin to the immune system and can be maintained in solution for six weeks without significant proteolytic degradation.

The invention relates to a reducible and degradable nano medicine-carrying micelle and a preparation method thereof, belonging to the fields of biomedical technology and nano medical technology. The reducible and degradable nano medicine-carrying micelle is characterized by having the following structural general formula: MPEG-CA-PCL, wherein the CA is poly-cystamine containing disulfide bonds, the MPEG is methoxy polyethylene glycol the molecular weight of which is 475-5000DA, and the PCL is polycaprolactone. The preparation method of the reducible and degradable nano medicine-carrying micelle comprises the following steps: (1) carrying out Michael addition to N,N'-bis (acrylic) cystamine and ethanolamine to obtain hydroxy poly-cystamine containing disulfide bonds, and then, carrying out ring opening reaction on caprolactone by utilizing a hydroxy group to obtain a CA-PCL polymer; and (2) mixing the CA-PCL polymer obtained in the step (1) and carboxyl-terminated MPEG, and adding paclitaxel (PTX) to prepare the nano medicine-carrying micelle through a dialysis method. The method is efficient and simple.

The invention discloses a method for directly extracting nickel in acidic chemical nickel plating waste solution, and relates to treatment of industrial waste solution. The invention provides the method for directly extracting nickel in the acidic chemical nickel plating waste solution, which can reduce the nickel-containing waste water treatment cost, improve the energy utilization of the nickel and achieve zero discharge of the acidic chemical nickel plating waste solution in place of the traditional chemical method, electro-dialysis method, electrolysis method, RO film separation method, pressure filtering method and the like. The method comprises the following steps of: injecting the acidic chemical nickel plating waste solution into an acidic chemical nickel plating waste solution tank; adding a catalytic reducer and a nickel metal extraction carrier into the acidic chemical nickel plating waste solution tank; depositing nickel ions, which are more than or equal to 97 percent in the acidic chemical nickel plating waste solution, on the nickel metal extraction carrier; and performing RO film reverse osmosis treatment and thermal evaporation treatment on the acidic chemical nickel residue after direct extraction of nickel so as to produce solid waste residue, namely the direct extraction of the nickel in the acidic chemical nickel plating waste solution is finished.

The present invention relates to a blood dialysis apparatus, in which a plural number of adequate dialysis therapy data (time-series data), wherein an estimated dry weight is closely similar to the dry weight, are memorized and thus it can be easily determined whether or not the subsequent dialysis therapy process achieves adequate therapeutic effects closely similar to the dry weight, and a blood dialysis method. A blood dialysis apparatus comprising: a dialytic treatment unit by which water is removed in accordance with the water removal amount determined based on an estimated dry weight and dialysis therapy is conducted; a BV detection unit (9) by which BV of the blood under extracorporeal circulation is measured at multiple points through time in the course of the dialysis therapy by using the dialytic treatment unit to thereby give time-series data; a determination unit (10) by which it is determined whether or not the estimated dry weight is closely similar to the dry weight after the completion of the dialytic therapy by using the dialytic treatment unit to thereby determine the adequacy of the dialysis therapy; and a memory unit (11) by which a plural number of the time-series data of the dialysis therapy having been judged as adequate by the determination unit (10) can be memorized.

The invention discloses a method for improving the flexibility of a polypeptide membrane by polylactic acid and polydioxanone. The method comprises the following steps: (1) adding polypeptide homopolymer, diisocyanate, a catalyst and a solvent into a drying reactor, stirring to react for 40-60 minutes at 50-55 DEG C, and removing excessive diisocyanate by a dialysis method to obtain polypeptide homopolymer containing -NCO-terminated group; (2) adding the polypeptide homopolymer containing -NCO-terminated group, a catalyst and a solvent into the drying reactor, adding polylactic acid monododecyl ether, and stirring to react for 50-70 minutes at 40-50 DEG C to obtain polypeptide-poly-polylactic acid diblock copolymer; (3) adding the polypeptide-poly-polylactic acid diblock copolymer, polydioxanone and a solvent into the drying reactor, stirring and mixing for 60-70 minutes at 40-50 DEG C, forming a membrane by using a tape casting method, and drying to obtain a target product. The preparation process is simple, and the flexibility of the modified membrane is greatly improved.

The present invention relates to hollow fiber membrane modules for use as “immersion modules” in filtration and / or dialysis methods, in particular whenever impairments due to fouling effects are expected from the use of liquids that are contaminated or lead to deposits. The hollow fiber membrane module comprises at least one housing and a plurality of tubular hollow fiber membranes which are arranged parallel to one another in and / or around the housing in a packing space, wherein the volumetric ratio of all the hollow fiber membranes arranged in the packing space to the packing space is less than 20%. Methods for producing such hollow fiber membrane modules are also provided.

A process for preparing the acetylcholinesterase used to detect the content of residual agricultural chemical in fruit and vegetable includes such steps as extracting the coarse liquid extract from animal blood, purifying by ammonium sulfate deposition method, removing ammonium sulfate by dialysis method, concentrating and drying. Its advantages are widely available raw material, low cost and high purity and sensitivity of product.