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32 results about "Phosvitin" patented technology

Phosvitin is one of the egg (commonly hen’s egg) yolk phosphoproteins known for being the most phosphorylated protein found in nature. Phosvitin isolation was first described by Mecham and Olcott in the year 1949. Recently it has been shown that disordered secondary structure of phosvitin orchestrates nucleation and growth of biomimetic bone like apatite.

Iron-enriched yolk powder and preparation method thereof

The invention belongs to the technical filed of agricultural by-product deep-processing and comprehensive utilization of by-products thereof, and relates to iron-enriched yolk powder and a preparation method thereof. The preparation method of the iron-enriched yolk powder comprises the following technological processes: preparing a main ingredient, preparing a phosvitin peptide iron compound, blending the main ingredient and the auxiliary ingredients, carrying out vacuum deodorization and cooling, and carrying out vacuum freeze-drying. Yolk liquid is used as the main ingredient; and maltose, pectin, milk powder, soybean powder and the phosvitin peptide iron compound are used as the auxiliary ingredients. The main ingredient and the auxiliary ingredients are processed so as to prepare the iron-enriched yolk powder which has the functions of supplementing iron, and is light yellow in color, uniform in texture, appropriate in taste, free of peculiar smell of the egg, and easy for the human body to absorb. The percentage by weight of each auxiliary ingredient compared with the main ingredient is as follows: 3-8% of the maltose, 5-10% of the pectin, 40-60% of the milk powder, 40-60% of the soybean powder, 2.6-3.8% of the phosvitin peptide iron compound, and 30-40% of water.
Owner:DALIAN POLYTECHNIC UNIVERSITY

Anticariogenic Proteins & Peptides & Saccharides

InactiveUS20080226565A1Attractive toxicological profileExcellent and enhanced protectionAntibacterial agentsOrganic active ingredientsEpsilon-PolylysineADAMTS Proteins
This Invention discloses new proteins, peptides and saccharides that have anticarogenic capabilities and that are characterized by the presence of one or more components that have the ability to form a complex with calcium ions: such as epsilon-polylysine that is conjugated with one or more bisphosphonyl-, biscarboxyl-, or 3-hydroxy-phthalate-groups or conjugated with casein phosphopeptide, phosvitin or with partially hydrolyzed phosvitin; such as partially hydrolized chitosan that is conjugated with one or more bisphosphonyl groups, casein phosphopeptide or with phosvitin or partially hydrolized phosvitin; such as bisphosphonylated and biscarboxylated proteins with at least 40% of amino acids consisting of lysine and a molecular weight of above 2 kD and such as polymerized casein phosphopeptide and partially hydrolized phosvitin. The products can be used in formulations to protect teeth and to treat the oral cavity. The Invention encompasses competent protein, peptide and saccharide structures, as well as production procedures and application conditions.
Owner:HUYBRECHTS LUCAS

Anticariogenic proteins and peptides and saccharides

This Invention discloses new proteins & peptides & saccharides that haue anti cariogenic capabilities and that are characterized by the presence of one or more components that haue the ability to form a complex with calcium Ions: such as epsilon-polylysine that is conjugated with one or more bisphosphonyl- , biscarboxyl-, or 3-hydroxyphthalate-groups or conjugated with Casein phosphopeptide, phosvitin or with partially hydrolyzed phosvitin; such as partially hydrolyzed chitosan that is conjugated with one or more bisphosphonyl groups, Casein phosphopeptide or with phosvitin or partially hydrolyzed phosvitin; such as bisphosphonylated and biscarboxylated proteins with at least 40% of amino acids consisting of lysine and a molecular weight of above 2 kD (2000 dalton) and such as polymerized Casein phosphopeptide and partially hydrolyzed phosvitin. Especially basic polymers that are conjugated with bisphosphonyl-groups, such as bisphosphonylated epsilon-polylysine, are strong protectors due to the simultaneous presence in one molecule of strong calcium-complexing- and strong acid buffering components. In addition, polylysines, such as epsilon-polylysine haue demonstrated antibacterial activity against a large variety of oral cavity bacteria including acid producing bacteria. It indicates it's relevance for the protection of teeth in particular and for control of the bacterial flora in the oral cavity in general. The products can be used in formulations to protect teeth and to treat the oral cavity: toothpastes, gels, mouth rinses, artificial saliva's ....for patients and healthy consumers. They haue an attractive toxicological profile compared to fluoride, and can be used in food systems; they act additionally to the action of fluoride. The use in combination with fluoride provides excellent and enhanced protection at minimum fluoride dosage. The Invention encompasses competent protein & peptide & saccharide structures, based an in-vitro and in-vivo experiments.
Owner:卢卡斯·海布雷赫特

Formula and preparation method of phosvitin phosphoeptide milk flavor calcium tablet

The invention relates to a formula and a preparation method of a phosvitin phosphoeptide milk flavor calcium tablet, belonging to the technical field of functional foods. The formula comprises main materials, a bulking agent, a flavoring agent, a disintegrating agent, a binding agent, a lubricant and an antioxidant, and the phosvitin phosphoeptide milk flavor calcium tablet comprises the following components in percentage by mass: 1-6 percent of phosvitin phosphoeptide, 30-40 percent of milk powder, 0.5-1.75 percent of calcium carbonate, 45-50 percent of bulking agent, 0.75-2 percent of flavoring agent, 10 percent of disintegrating agent, 5-15 percent of binding agent, 0.5-1 percent of lubricating agent and 0.25-0.5 percent of antioxidant. The invention has the advantages that the calcium tablet is a double-functional nutritious health care food with the functions of both calcium supplement and calcium absorption promotion, consumers can acquire the health care effect of calcium supplement when enjoying palatableness, and meanwhile, the toxic side effect caused by using V-D calcium supplement is avoided.
Owner:JILIN UNIV

Extraction and purification method of phosvitin in eggs

The invention discloses an extraction and purification method of phosvitin in eggs, which can be directly applied to foods, drugs and other industries with higher requirement on purity, produces products with high purity, is easy to operate and produce industrially. The method comprises the following steps of: removing water-soluble substances from yolk in an egg; degreasing with ethanol; extracting with high-concentration salt liquid to obtain a phosvitin coarse product; purifying the phosvitin coarse product by utilizing an anion exchange chromatography, and removing salt to obtain high-purity phosvitin. According to the method, the phosvitin is purified by adopting the anion exchange chromatography, so that the purity of the phosvitin can be greatly improved, and the reclamation of the phosvitin is high. Each step in the process is easy to operate, the equipment is low in cost, industrial production can be realized easily, and the phosvitin can be directly applied to the industry of foods and drugs.
Owner:TIANJIN UNIV OF COMMERCE

Edible aerated oil-and-water emulsion

One aspect of the invention relates to an aerated water-continuous oil-and-water emulsion having an overrun in the range of 10-500%, said emulsion comprising:5-90 wt. % of an oil phase;10-95 wt. % of an aqueous phase;0.3-30% by weight of the aqueous phase of one or more egg yolk granule proteins selected from high density lipoprotein (HDL) and phosvitin; andfrom 0.05 up to 10% by weight of the aqueous phase of one or more egg yolk plasma proteins selected from low density lipoprotein (LDL) and livetin;wherein the weight ratio of egg yolk granule proteins to egg yolk plasma proteins exceeds 1:1.The aerated emulsions according to the present invention can easily be produced at a high overrun. Furthermore, the foam structure of these emulsions remains surprisingly stable during shelf life.
Owner:CONOPCO INC D B A UNILEVER

Method for jointly extracting IgY, phosvitin, lecithin, egg oil and skim yolk powder from egg yolk

The invention discloses a method for jointly extracting IgY, phosvitin, lecithin, egg oil and skim yolk powder from egg yolk. The method comprises the following steps: firstly, diluting yolk fluid by using water and centrifuging; salting out the acquired supernatant and performing polyethylene glycol sediment, thereby extracting IgY; for the yolk pulp on the bottom layer, removing water from the yolk pulp by adopting ethyl alcohol, extracting total yolk lipid by utilizing an ethyl alcohol and ethyl acetate mixed solvent and treating by utilizing ethyl acetate, thereby acquiring two products of lecithin and egg oil; treating the egg white by utilizing a sodium chloride solution after removing the lipid, thereby acquiring the phosvitin. According to the method, the technological operation is simple and convenient, environment-friendly and non-toxic effects are achieved, the production cost is low, the product purity and yield are higher and a technical support is supplied for the comprehensive utilization of yolk.
Owner:HUAZHONG AGRI UNIV

Phosvitin phosphopeptide enrichment method

The invention discloses a phosvitin phosphopeptide enrichment method and belongs to the field of extraction, purification and separation. The phosvitin phosphopeptide enrichment method realizes enrichment of phosvitin phosphopeptide by mesoporous / macroporous TiO2 as an adsorption material. The prepared phosvitin phosphopeptide has N / P of 4.5. Mesoporous / macroporous TiO2 can utilize metal oxide TiO2 specific affinity effects on a phosphate group. Through high specific surface area and a good tunnel structure of the mesoporous-macroporous structure, a large amount of active binding sites are provided. Under enrichment conditions of pH of 2.5-4, an initial polypeptide concentration of 12-20mg / mL and adsorption time of 5-20min, the PPPs has purity of 4.3-4.8 and the corresponding adsorption quantity is in a range of 42-53mg / mL. The phosvitin phosphopeptide enrichment method has simple processes, is fast and effective and is conducive to industrial production.
Owner:江苏中农鹏源生物科技有限公司

Method for separating phosvitin from fowl eggs

InactiveCN101133774AAffect biocompatibilityAffect biosecurityProtein composition from eggsClimate change adaptationFowlWater soluble
The present invention relates to a method for separating phosritin from egg, belonging to the field of food engineering technology. Said method includes the following technology. Said method includes the following steps: firstly, taking out egg yolk from egg, making its egg-yolk lipoprotein be separated from water-soluble egg-white protein to obtain crude egg-yolk lipoprotein, mixing the crude egg-yolk lipoprotein with an iron-based inorganic nano material with iron atom affinity activity site to make them produce interaction and make the correspondent phosritin be adsorbed on the iron-based nano material, finally, making them undergo the process of boiling treatment so as to obtain the invented phosritin.
Owner:SHANGHAI JIAO TONG UNIV

Industrial preparation method of phosvitin phosphopeptide and yolk polypeptide

The invention discloses an industrial preparation method of phosvitin phosphopeptide and yolk polypeptide, and belongs to the technical field of biology. The preparation method takes skim egg yolk powder, especially the skim egg yolk powder with severe degeneration, loss of functionality and severely reduced values after the industrial extraction of lecithin, as a raw material; through twice enzymolysis, a ceramic membrane is used to separate yolk polypeptide and phosvitin after first enzymolysis, and secondary enzymolysis is performed after the alkali treatment of the phosvitin, so that the yolk polypeptide and the phosvitin phosphopeptide can be simultaneously prepared. Through the method, the skim egg yolk powder can be fully utilized, the values of the low-value skim egg yolk powder after the industrial extraction of the lecithin can be especially and effectively enhanced, and the industrial production of the yolk polypeptide and the phosvitin phosphopeptide can be synchronously realized.
Owner:JIANGNAN UNIV

Co-production and separation method of multiple bioactive substances in yolk

The invention discloses a co-production and separation method of multiple bioactive substances in yolk. The method includes (1), cholesterol extraction, (2), lecithin extraction and (3), phosvitin extraction. The method has the advantages that yolk oil is extracted by a supercritical CO2 extraction method, and lecithin is extracted by an alcohol extraction method, so that extraction ratio of cholesterol is increased, and purity of the egg yolk lecithin is improved; nontoxic edible ethyl alcohol serves as a solvent during lecithin extraction, so that edible safety of products is improved; through enzymolysis-assisted extraction, breakage of covalent bonds between lipids and egg white is benefited, extraction rate of the egg yolk lecithin is increased, and production cycle is shortened; phosvitin desalination is performed according to a ultrafiltration technology, and product purity is further improved; the method is simple in process, convenient to operate and suitable for large-scale industrial production.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES

Method for catalytically synthesizing biomimetic material hydroxyapatite from phosvitin and polypeptide thereof

The invention discloses a method for catalytically synthesizing a biomimetic material hydroxyapatite from phosvitin and a polypeptide thereof. The method includes following steps: dissolving a calcium salt and a phosphate, performing a reaction under a neutral condition with catalysis of the phosvitin and the polypeptide thereof, and collecting generated precipitates in the reaction to obtain the biomimetic material hydroxyapatite. In the invention, by means of bioactivities of the phosvitin and athepolypeptide thereof, a crystal phase transition during synthesis of the phosvitin is greatly promoted, a crystal phase transition time is reduced from 16 hours to 5-15 min and a conversion speed is increased by 48-216 times. The phosvitin polypeptide is high in purity and is strong in catalytic activity.
Owner:HUAZHONG AGRI UNIV

Preparation process of channel catfish roe extract

The invention provides a preparation technology of channel catfish roe extract. The preparation technology comprises the following steps that channel catfish roes are subjected to fishy smell removal, decoloration and impurity removal treatment through a three-removal treatment solution; rinsing the ietalurus punetaus roes subjected to three-stage removal treatment to obtain clean ietalurus punetaus roes without mucus; the method comprises the following steps: grinding rinsed clean channel catfish roes, adding sterile pure water, adding channel catfish roe hydrolysis compound enzyme, carrying out enzyme hydrolysis, and filtering to obtain an enzymatic hydrolysate; extracting the dissolved enzymatic hydrolysate, performing centrifugal treatment, and taking upper suspension slurry and supernate; freezing and drying to obtain a roe extract; the channel catfish egg extract prepared by the preparation method disclosed by the invention can retain the main nutritional ingredients of channel catfish eggs: yolk proplasm, vitelline lipoprotein peptide, phosvitin peptide, vitelline globulin peptide, five nucleic acids, 18 essential chloro acids, omega-3 and omega-6 fatty acids, multivitamins, minerals, trace elements and the like.
Owner:广西金海盈高新技术有限公司 +1

Phosvitin-derived calcium chelating peptide, and peptide calcium chelate and application thereof, as well as application of peptide calcium chelate

The invention belongs to the field of biological small molecular active peptides, and particularly relates to phosvitin-derived calcium chelating peptide, and a peptide calcium chelate and applicationthereof, as well as an application of the peptide calcium chelate. The calcium chelating peptide consists of nine amino acids, the sequence is DEEENDQVK, when the calcium chelating peptide DEEENDQVKprovided by the invention is used for preparing the peptide calcium chelate, the calcium binding rate and the yield of the chelating peptide calcium are high, the synthetic peptide calcium binding rate is 97.00 + / -1.42%, the yield of the chelating peptide calcium is 87.76 + / -1.91%, and the calcium ion chelating amount is 151.10 + / -3.57 mg / g. The digestive absorption rate and retention rate of thepeptide calcium chelate DEEENDQVK-Ca provided by the invention are higher than those of other organic calcium and CaCO3, so that calcium absorption is facilitated; and the high-valued utilization of egg products is facilitated, and the calcium deficiency phenomenon of people is obviously improved.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY

Method for jointly extracting multiple kinds of yolk protein based on water phase separation and product

The invention provides a method for jointly extracting multiple kinds of yolk protein based on water phase separation and a product. The method includes the steps that (1), fresh yolk is taken, a water phase solution with the volume 0.5-5 times that of the yolk is added for dilution, and the material is stirred, and is subjected to standing or is centrifugally layered; an upper-layer component A,a middle-layer component B and a lower-layer component C are obtained, wherein the water phase solution is polysaccharide matter with the mass percentage concentration of 0.1-0.5% or 2-8% of a water solution of low-molecular polymers; (2), the component A is taken, and yolk low-density lipoprotein is prepared; (3), the component B is taken, and yolk immune globulin is prepared; (4), the componentC is taken, and phosvitin and yolk high-density lipoprotein are prepared. The process is simple and short, the operation is easy, four kinds of yolk protein can be jointly extracted, the extracting efficiency is high, and the unit raw material output is high; extraction is carried out based on water phase separation, reagents used in the method are all food-grade or medicine-grade components, andthe reagents are green and safe; the treatment process is mild, and the functional characteristics and the bio-activity of the protein can be reserved to the maximum degree.
Owner:CHENGDU UNIV

A kind of industrial preparation method of phosvitin phosphopeptide and yolk polypeptide

The invention discloses an industrial preparation method of phosvitin phosphopeptide and yolk polypeptide, belonging to the field of biotechnology. The present invention uses defatted egg yolk powder, especially defatted egg yolk powder that is seriously denatured, lost in functionality, and seriously reduced in value after industrialized extraction of lecithin, as a raw material, through two enzymatic hydrolysis, and after the first enzymatic hydrolysis, a ceramic membrane is used to separate the egg yolk polypeptide and phosvitin, the second enzymolysis is carried out after the phosvitin is treated with alkali, so as to simultaneously prepare the phosvitin polypeptide and the phosvitin phosphopeptide. The method of the invention can make full use of the defatted egg yolk powder, especially can effectively improve the value of the low-value defatted egg yolk powder after industrial lecithin extraction, and simultaneously realize the industrial production of yolk polypeptide and phosvitin phosphopeptide.
Owner:JIANGNAN UNIV

Edible aerated oil-and-water emulsion

One aspect of the invention relates to an aerated water- continuous oil-and-water emulsion having an overrun in the range of 10-500%, said emulsion comprising: 5-90 wt.% of an oil phase; 10-95 wt.% ofan aqueous phase; 0.3-30% by weight of the aqueous phase of one or more egg yolk granule proteins selected from high density lipoprotein (HDL) and phosvitin; and from 0.05 up to 10% by weight of theaqueous phase of one or more egg yolk plasma proteins selected from low density lipoprotein (LDL) and livetin; wherein the weight ratio of egg yolk granule proteins to egg yolk plasma proteins exceeds1:1. The aerated emulsions according to the present invention can easily be produced at a high overrun. Furthermore, the foam structure of these emulsions remains surprisingly stable during shelf life.
Owner:UNILEVER NV

Application of phosvitin

The invention provides an application of phosvitin to inhibition of helicobacter pylori. According to the application of the phosvitin, the antibacterial potential of the phosvitin which is a functional food-borne substance is excavated, and the application value of the phosvitin is further improved.
Owner:NORTHWEST A & F UNIV

Edible oil-in-water emulsion comprising egg yolk components

The present invention provides a method of preparing an edible oil-in-water emulsion having a pH in the range of 3.0 to 5.0 and comprising 30-78 wt. % of oil, 65-20 wt. % water and 0.5-6 wt. % egg yolk proteinaceous component by dry weight, said egg yolk proteinaceous component consisting of a combination of low-density lipoprotein (LDL), livetin, high-density lipoprotein (HDL), and phosvitin, said method comprising the steps of:a. increasing the pH of a liquid aqueous mixture comprising a first egg yolk plasma fraction to a pH of at least 9 and keeping the liquid aqueous mixture at said pH for 1-300 minutes followed by decreasing the pH of the mixture to a pH of 7 or less to produce an alkaline treated egg yolk plasma liquid comprising alkaline treated egg yolk plasma fraction;b. preparing a first pre-emulsion by combining oil, water, egg yolk granules fraction and optionally a second egg yolk plasma fraction;c. combining the alkaline treated egg yolk plasma liquid with the pre-emulsion to produce a second pre-emulsion; andd. homogenizing the second pre-emulsion to obtain an oil-in-water emulsion;wherein the egg yolk proteinaceous component of the oil-in-water emulsion has the following composition:60-75 wt. % LDL;8-14 wt. % livetin;11-18 wt. % HDL;2-5 wt. % phosvitin.
Owner:CONOPCO INC D B A UNILEVER

A kind of extraction method of chicken embryo egg yolk phosphoprotein phosphopeptide

The invention discloses an extraction method of embryonated egg phosvitin phosphopeptides, which belongs to the technical field of food additives. According to the invention, by collecting embryonated eggs incubated for a certain number of days, and through the approaches of tissue homogenate, centrifugal sedimentation, degreasing and filtering, and the like, phosvitin is extracted; then, under ultrahigh pressure aided conditions, carrying out hydrolysis on the phosvitin; and carrying out ultrafiltration on the obtained product so as to obtain a micromolecule combination, and drying the obtained product so as to obtain a phosphopeptide product. Compared with traditional processes for preparing phosphopeptides from ordinary eggs, the method fully uses the enzymolysis and dephosphorization of phosvitin by embryonated egg endogenous enzymes, thereby eliminating the steps of artificial dephosphorization and the like, so that the process time is greatly shortened, and the cost is saved. The obtained phosphopeptides product has an effect of promoting mineral absorption, therefore, the phosphopeptides product can be developed as a novel functional food, or as a natural food additive for promoting mineral absorption, added into functional foods.
Owner:JIANGNAN UNIV

Method for Catalytic Synthesis of Biomimetic Material Hydroxyapatite Using Phosvitin and Its Polypeptide

The invention discloses a method for catalytically synthesizing a biomimetic material hydroxyapatite from phosvitin and a polypeptide thereof. The method includes following steps: dissolving a calcium salt and a phosphate, performing a reaction under a neutral condition with catalysis of the phosvitin and the polypeptide thereof, and collecting generated precipitates in the reaction to obtain the biomimetic material hydroxyapatite. In the invention, by means of bioactivities of the phosvitin and athepolypeptide thereof, a crystal phase transition during synthesis of the phosvitin is greatly promoted, a crystal phase transition time is reduced from 16 hours to 5-15 min and a conversion speed is increased by 48-216 times. The phosvitin polypeptide is high in purity and is strong in catalytic activity.
Owner:HUAZHONG AGRI UNIV

a tio 2 Preparation of diatomite composite material and method for purifying phosvitin phosphopeptide

A new type of TiO 2 The invention relates to the preparation of diatomite composite material and the method for purifying phosvitin phosphopeptide, which belong to the technical field of chemical composite material preparation and biological active substance purification. The invention provides an improved precipitation method for preparing loaded nano TiO 2 method, and use this loaded composite material as an adsorption carrier to separate and purify phosvitin phosphopeptide. The present invention selects the diatomite material with larger specific surface area as the loading matrix, and TiO 2 Deposited on the surface of diatomite by chemical co-precipitation method, the prepared TiO was controlled by controlling the experimental conditions 2 The particle size and crystal form of the prepared composites have larger specific surface area and better coverage. Applying it to the separation and purification of phosvitin phosphopeptide can improve its purification efficiency and adsorption capacity. At the same time, it can also be applied to the research of phosphoproteomics for the identification of protein phosphorylation sites.
Owner:广东京豪生物制药有限公司

Edible oil-in-water emulsion comprising egg yolk components

The invention relates to a method of producing an edible oil-in-water emulsion having a pH in the range of 3.0 to 5.0 and comprising 30-78 wt. % of oil, 65-20 wt. % water and 0.5-6 wt. % hen's egg yolk proteinaceous component by dry weight, said egg yolk proteinaceous component consisting of a combination of low-density lipoprotein (LDL), livetin, high-density lipoprotein (HDL), and phosvitin, and the method comprises the steps of:heating a liquid aqueous mixture comprising a first egg yolk plasma fraction to produce heat treated egg yolk plasma liquid;a. preparing a first pre-emulsion by combining oil, water, egg yolk granules fraction and optionally a second egg yolk plasma fraction;b. combining the heat treated egg yolk plasma liquid with the pre-emulsion to produce a second pre-emulsion;c. Adding one or more acidulants; andd. homogenizing the second pre-emulsion to obtain an oil-in-water emulsion;wherein the egg yolk proteinaceous component of the oil-in-water emulsion has the following composition:60-75 wt. % LDL;8-14 wt. % livetin;11-18 wt. % HDL;2-5 wt. % phosvitin.
Owner:CONOPCO INC D B A UNILEVER

Biological indicator based on phosvitin as well as preparation method and application of biological indicator

The invention belongs to the technical field of sterilization indication, and particularly relates to a biological indicator based on phosvitin. The biological indicator is a phosvitin aqueous solution or a phosvitin aqueous solution containing a phosvitin iron compound. The invention provides a preparation method and application of the biological indicator based on phosvitin. According to the biological indicator based on phosvitin, the problem that monitoring of the steam sterilization effect is blank is solved, biological culture is not needed, the conclusion whether the sterilization effect is qualified or not can be obtained in time, the using and issuing period of sterilization instruments is shortened, the distribution and use efficiency of sterilization instruments is improved, the monitoring cost is low, and the biological indicator is suitable for sterilization monitoring of food products.
Owner:HUAZHONG AGRI UNIV
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