Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

a tio 2 Preparation of diatomite composite material and method for purifying phosvitin phosphopeptide

A phosvitin and composite material technology is applied in the fields of composite material preparation and separation and purification of biologically active substances, and can solve the problems of complex separation process, short production cycle, numerous steps, etc., and achieve high yield and purity and regular morphology. , the effect of high activity retention rate

Active Publication Date: 2016-09-07
广东京豪生物制药有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using prepared TiO 2 / Diatomite composite material separation and purification of phosvitin phosphopeptide, easy operation, low cost, short production cycle, and easy large-scale production of phosvitin phosphopeptide, which solves the complicated separation process of traditional phosvitin phosphopeptide , many steps, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • a tio <sub>2</sub> Preparation of diatomite composite material and method for purifying phosvitin phosphopeptide
  • a tio <sub>2</sub> Preparation of diatomite composite material and method for purifying phosvitin phosphopeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Weigh a certain amount of diatomite and disperse it in dilute hydrochloric acid with a solid-to-liquid ratio of 1:10, stir for 3 hours, then adjust the pH value to 5.0, then filter, wash, and calcinate to obtain a relatively pure carrier diatomite, wherein The concentration of dilute hydrochloric acid is 2M and the pH value is 4. Weigh a certain mass of purified diatomite, add hydrochloric acid with a solid-to-liquid ratio of 1:10 and a concentration of 2M, stir for 30 minutes, and then add a certain volume of TiCl 4 solution, continue to stir for 2h, then adjust the pH value of the reaction system to 4, and then react for 2h under stirring conditions to terminate the reaction. Filter and wash the material with deionized water until the filtrate is free of Cl ions, using a stirring rate of 500 rpm. The above materials were dried and calcined at 800°C for 5h to obtain TiO 2 / Diatomaceous earth composite material.

[0025] Use the acetone-ethanol extraction method to r...

Embodiment 2

[0028] Weigh a certain amount of diatomite and disperse it in dilute hydrochloric acid with a solid-to-liquid ratio of 1:10, stir and react for 3 hours, then adjust the pH value to 4.0, then filter, wash, and calcinate to obtain relatively pure carrier diatomite, wherein The concentration of dilute hydrochloric acid is 2M and the pH value is 4. Weigh a certain quality of purified diatomite, add hydrochloric acid with a solid-liquid ratio of 1:10 and a concentration of 0.1M, stir for 30 minutes, and then add a certain volume of TiCl 4Solution, continue to stir for 2h, then adjust the pH value of the reaction system to 6, react under stirring conditions for 2h, terminate the reaction, filter, wash the material with deionized water until the filtrate does not contain Cl ions, and the stirring rate used is 400rpm. The above materials were dried and calcined at 500°C for 5h to obtain TiO 2 / Diatomaceous earth composite material.

[0029] Use the acetone-ethanol extraction method ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A new type of TiO 2 The invention relates to the preparation of diatomite composite material and the method for purifying phosvitin phosphopeptide, which belong to the technical field of chemical composite material preparation and biological active substance purification. The invention provides an improved precipitation method for preparing loaded nano TiO 2 method, and use this loaded composite material as an adsorption carrier to separate and purify phosvitin phosphopeptide. The present invention selects the diatomite material with larger specific surface area as the loading matrix, and TiO 2 Deposited on the surface of diatomite by chemical co-precipitation method, the prepared TiO was controlled by controlling the experimental conditions 2 The particle size and crystal form of the prepared composites have larger specific surface area and better coverage. Applying it to the separation and purification of phosvitin phosphopeptide can improve its purification efficiency and adsorption capacity. At the same time, it can also be applied to the research of phosphoproteomics for the identification of protein phosphorylation sites.

Description

technical field [0001] The invention relates to the technical field of composite material preparation and separation and purification of biologically active substances, in particular to a preparation of a composite material using metal oxides to load active carriers, and a method for separating and purifying phosvitin phosphopeptides. Background technique [0002] Phosvitin is the main phosphoprotein in egg yolk. Its primary structure contains 217 amino acid residues, 124 of which are serine residues, accounting for about 56% of the total amino acid residue content. Phosvitin contains a high net negative charge of about -179, which is the active carrier of cationic protein. Numerous studies have shown that phosvitin can interact with Ca 2+ , Mg 2+ , Mn 2+ ,Co 2+ ,Sr 2+ , Fe 2+ , Fe 3+ Wait for the combination. On the other hand, since phosvitin binds firmly with metal ions and is not easily hydrolyzed, its biological potency is low, which is not conducive to nutrient...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/14B01J20/30C12P21/06C07K1/14
Inventor 杨严俊苏宇杰张阳
Owner 广东京豪生物制药有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com