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Phosvitin-derived calcium chelating peptide, and peptide calcium chelate and application thereof, as well as application of peptide calcium chelate

A yolk protein and calcium chelation technology, applied in the field of biological small molecule active peptides, can solve the problems of limiting the application of calcium supplement products, unclear binding sites and mechanism of action, etc., to promote high-value utilization, improve Effects of calcium deficiency

Active Publication Date: 2021-01-22
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

PV is the protein with the highest degree of phosphorylation in nature, which can bind a large amount of calcium ions with strong affinity, but its binding site and mechanism of action are still unclear, which limits its application as a calcium supplement product

Method used

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  • Phosvitin-derived calcium chelating peptide, and peptide calcium chelate and application thereof, as well as application of peptide calcium chelate
  • Phosvitin-derived calcium chelating peptide, and peptide calcium chelate and application thereof, as well as application of peptide calcium chelate
  • Phosvitin-derived calcium chelating peptide, and peptide calcium chelate and application thereof, as well as application of peptide calcium chelate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The acquisition of embodiment 1 calcium chelating peptide DEEENDQVK

[0045]The calcium chelating peptide DEEENDQVK provided by the present invention can be prepared by artificial chemical synthesis, such as using chemical solid-phase synthesis according to the conventional techniques in the art; it can also be extracted from egg yolk liquid, and further processed by proteolysis and separation. Obtained after purification and other steps.

[0046] (1) Extract phosvitin

[0047] Collect the egg yolk liquid into a beaker, dilute it with distilled water of the same mass, stir and mix for 1 hour, then centrifuge to collect the precipitate, add the same mass of 0.17mol / L NaCl solution and continue to stir for 1 hour, and centrifuge again to collect the precipitate to obtain egg yolk particles.

[0048] The obtained egg yolk pellets were taken, and a 1.74 mol / L NaCl solution with a mass volume ratio of 10 times was added thereto, and stirred until completely dissolved. Afte...

Embodiment 2

[0059] Example 2 Characterization of Calcium Chelating Peptide DEEENDQVK and Peptide Calcium Chelate DEEENDQVK-Ca

[0060] 1. Synthesis of Calcium-Binding Peptides

[0061] Synthesis of purified peptides were produced by Shanghai Gill Biochemical Co., Ltd. (Shanghai, China) using solid-phase peptide synthesis. The purity and molecular weight of the synthetic peptide were determined by reverse-phase high-performance liquid chromatography equipped with SHIMADZU Inertsil ODS-SP (4.6mm×250mm, 5μm) and LC-ESI-MS / MS, and the calcium chelation activity of the synthetic peptide was determined by the aforementioned method .

[0062] DEEENDQVK was synthesized by solid-phase synthesis, and the purity of the synthetic peptide was determined to be 98.24% ( Figure 5 ). It was verified that the calcium binding rate of the synthetic peptide was 97.00±1.42%, and the calcium yield of the chelating peptide was 87.76±1.91%. The amount of calcium ion chelation is 151.10±3.57mg / g.

[0063] 2....

Embodiment 3

[0080] The mensuration of embodiment 3 peptide calcium chelate bioavailability

[0081] Simulate the environment of food in the gastrointestinal tract (including pepsin-HCl and pancreatin-bile salts), measure the solubility of soluble mineral elements and the permeability through a semi-permeable membrane with a certain pore size, and evaluate the bioavailability of mineral elements . Three calcium preparations (CaCO 3 , calcium gluconate and the peptide calcium chelate DEEENDQVK-Ca prepared in Example 1) in the digestion of simulated gastrointestinal tract environment (%) and permeability (%) with the change of digestion time (h).

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Abstract

The invention belongs to the field of biological small molecular active peptides, and particularly relates to phosvitin-derived calcium chelating peptide, and a peptide calcium chelate and applicationthereof, as well as an application of the peptide calcium chelate. The calcium chelating peptide consists of nine amino acids, the sequence is DEEENDQVK, when the calcium chelating peptide DEEENDQVKprovided by the invention is used for preparing the peptide calcium chelate, the calcium binding rate and the yield of the chelating peptide calcium are high, the synthetic peptide calcium binding rate is 97.00 + / -1.42%, the yield of the chelating peptide calcium is 87.76 + / -1.91%, and the calcium ion chelating amount is 151.10 + / -3.57 mg / g. The digestive absorption rate and retention rate of thepeptide calcium chelate DEEENDQVK-Ca provided by the invention are higher than those of other organic calcium and CaCO3, so that calcium absorption is facilitated; and the high-valued utilization of egg products is facilitated, and the calcium deficiency phenomenon of people is obviously improved.

Description

Technical field: [0001] The invention belongs to the field of biological small molecular active peptides, and in particular relates to a calcium chelating peptide derived from phosvitin, a peptide calcium chelate and applications thereof. Background technique: [0002] Calcium is the main element necessary for the human body, accounting for about 1.5% to 2.2% of body weight. About 99 percent of calcium is found in bones as calcium phosphate, which plays a key role in maintaining bone strength. In addition, calcium is known as the second messenger of signal transmission and plays an important role in maintaining normal physiological functions of the human body such as nerve conduction, muscle contraction and blood clotting. Hypocalcemia occurs when calcium levels in the body get too low. Symptoms include numbness in the fingers and toes, muscle cramps, irritability, mental impairment, and muscle spasms. [0003] Calcium supplements currently on the market mainly include ino...

Claims

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Application Information

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IPC IPC(8): C07K14/465C07K1/107A23L33/165A61K38/17A61P9/12A61P3/14
CPCC07K14/465A23L33/165A61P9/12A61P3/14A23V2002/00A61K38/00A23V2200/30A23V2250/1578
Inventor 张晓维贾琦巩婷婷司凯刘会平
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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