The invention discloses a co-delivery nano-carrier of drugs and genes, a preparation method and an application thereof; and particularly relates to a co-delivery nano-carrier TCPL-siRNA-PPX which can carry a chemotherapeutic
drug and a genetic
drug simultaneously. The
drug delivery system is composed of a
polymer prodrug carrier TCPL, siRNA and a multifunctional polyanionic polyer PPX through
electrostatic adsorption among the components in a self-
assembly manner. The co-delivery nano-carrier can achieve targetedly delivery of the drug and the
gene to the same tumor
cell, release the siRNA at the
cytoplasm,
silence Bcl-2
protein, promote apotosis and relieve inhibition of the Bcl-2 on
lonidamine, and can deliver the chemotherapeutic drug,
lonidamine, which is
mitochondrion-acted to mitochondria so that the chemotherapeutic drug and the
gene can synergistically trigger the apotosis of a mitochondrial pathway and kill
tumor cells cooperatively. Through in-vivo and in-vitro activity evaluation, the
drug delivery system is proved to be better than
drug delivery carriers which deliver single components respectively at the same time, can significantly improve the anti-
cancer activity of the drug and the
gene and has a definite synergistic
treatment effect.