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Preparation method of targeting and photo-thermal integrated erythrocyte bionic nanoparticles

A red blood cell and nanoparticle technology, applied in the field of preparation of red blood cell bionic nanoparticles, can solve potential cell or genotoxicity, weaken anti-tumor effect and other problems, achieve active targeting, improve therapeutic effect, and increase stability Effect

Inactive Publication Date: 2018-12-28
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, traditional nanoparticles are easy to adhere to proteins, lipids or other biomolecules to form "protein rings", which will affect their behavior in the body. The blood is quickly cleared, which not only weakens its anti-tumor effect, but some nanomaterials also have potential cell or genotoxicity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1) Take several 6-week-old ICR mice, use capillary tubes to collect blood from eyeballs, add heparin to the blood for anticoagulation, centrifuge the obtained whole blood at 4°C, 2000 r / min for 10 min, and remove the serum and other substances in the upper layer For the blood cell layer, the obtained erythrocyte pellet was washed 3 times with cold 1×PBS and set aside;

[0047] 2) Add cold 0.25×PBS equivalent to 60 times the volume of red blood cell pellet to the red blood cell pellet obtained in step 1) for hypotonicity for 2 h. Then centrifuge at 10,000 r / min for 15 min, discard the upper liquid, and the pale pink precipitate in the lower layer is the erythrocyte membrane, then wash with 0.25×PBS repeatedly for 3 times to make the extracted erythrocyte membrane more pure, and set aside;

[0048] 3) Dissolve doxorubicin in water to prepare a doxorubicin solution with a concentration of 5%; dissolve polylactic acid-glycolic acid copolymer in dichloromethane to prepare a ...

Embodiment 2

[0059] 1) Take several 7-week-old ICR mice, use capillary tubes to collect blood from eyeballs, add heparin to the blood for anticoagulation, and centrifuge the obtained whole blood at 4°C for 10 minutes at 2000 r / min to remove the serum and other substances in the upper layer For the blood cell layer, the obtained erythrocyte pellet was washed 4 times with cold 1×PBS and set aside;

[0060] 2) Add cold 0.25×PBS equivalent to 60 times the volume of red blood cell pellet to the red blood cell pellet obtained in step 1) and hypotonic for 2.5 h. Then centrifuge at 10,000 r / min for 17 min, discard the upper liquid, and the pale pink precipitate in the lower layer is the erythrocyte membrane, then wash with 0.25×PBS four times to make the extracted erythrocyte membrane more pure, and set aside;

[0061] 3) Dissolve doxorubicin in water to prepare a doxorubicin solution with a concentration of 5%; dissolve polylactic acid-glycolic acid copolymer in dichloromethane to prepare a polyl...

Embodiment 3

[0072] 1) Take several 8-week-old ICR mice, use capillary tubes to collect blood from eyeballs, add heparin to the blood for anticoagulation, centrifuge the obtained whole blood at 4°C, 2000 r / min for 10 minutes, and remove the serum and other substances in the upper layer For the blood cell layer, the obtained erythrocyte pellet was washed 5 times with cold 1×PBS and set aside;

[0073] 2) Add cold 0.25×PBS equivalent to 60 times the volume of red blood cell pellet to the red blood cell pellet obtained in step 1) and hypotonic for 3 h. Then centrifuge at 10,000 r / min for 20 min, discard the upper liquid, and the pale pink precipitate in the lower layer is the erythrocyte membrane, and then wash it repeatedly with 0.25×PBS for 5 times to make the extracted erythrocyte membrane more pure, and set aside;

[0074] 3) Dissolve doxorubicin in water to prepare a doxorubicin solution with a concentration of 5%; dissolve polylactic acid-glycolic acid copolymer in dichloromethane to pr...

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Abstract

The invention relates to the field of pharmacy and provides a preparation method of targeting and photo-thermal integrated erythrocyte bionic nanoparticles. The method comprises the following steps: firstly, extracting erythrocyte membranes, then loading indocyanine green to a polylactic acid-hydroxyl acetic acid solution, burying adriamycin amycin to the system, and preparing an indocyanine greenpolylactic acid-hydroxyl acetic acid solution buried with adriamycin amycin through a secondary emulsification-solvent evaporation method; then carrying out a reaction on folic chitosan oligosaccharide and the indocyanine green polylactic acid-hydroxyl acetic acid solution to obtain an adriamycin amycin-folic chitosan oligosaccharide-indocyanine green-polylactic acid-hydroxyl acetic acid solution; and finally, extruding the adriamycin amycin-folic chitosan oligosaccharide-indocyanine green-polylactic acid-hydroxyl acetic acid solution into the erythrocyte membranes to obtain the erythrocyte bionic nanoparticles.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to a preparation method of red blood cell bionic nanoparticles integrating targeting and light and heat. Background technique [0002] Cancer (malignant tumor) is one of the refractory diseases that seriously endanger human health, with high morbidity and mortality every year. Therefore, the study of tumor formation and treatment methods has become the research focus and hotspot of researchers. Polymer nanoparticles are widely used in drug delivery due to their excellent biocompatibility and degradability, high drug loading and drug delivery efficiency, and good cycle stability. system. [0003] However, traditional nanoparticles are easy to adhere to proteins, lipids or other biomolecules to form "protein rings", which will affect their behavior in the body. The rapid clearance in the blood not only weakens its anti-tumor effect, but some nanomaterials also have potential cell or genot...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K9/50A61K9/107A61K47/46A61K47/34A61K47/22A61K47/36A61K31/704A61P35/00
CPCA61K9/1075A61K9/5068A61K31/704A61K41/0052A61K47/22A61K47/34A61K47/36A61P35/00
Inventor 王秉姚舒婷单心怡华称祥胡智文万军民
Owner ZHEJIANG SCI-TECH UNIV
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