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Method for jointly extracting IgY, phosvitin, lecithin, egg oil and skim yolk powder from egg yolk

A high yolk protein and combined extraction technology is applied in the field of deep processing of egg products, which can solve the problems of complex composition of egg yolk, difficulty in making the best use of everything, and little research on comprehensive utilization of egg yolk, etc. The effect of high oil lift

Active Publication Date: 2017-11-24
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Due to the complex composition of egg yolk, it is difficult to make the best use of it
At present, research mainly focuses on the separation and extraction of single bioactive components in egg yolk, and there is little research on the comprehensive utilization of egg yolk

Method used

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  • Method for jointly extracting IgY, phosvitin, lecithin, egg oil and skim yolk powder from egg yolk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Take 500ml of the egg yolk stock solution, add 9 times the volume of distilled water to dilute, adjust the pH=5.5, stir evenly, put it in a refrigerator at 4°C overnight, take it out, and centrifuge it at 10000rpm for 15min to obtain the supernatant A and the egg yolk pulp; then Ammonium sulfate and sodium chloride were added to the supernatant A to precipitate IgY, so that the final weight concentrations of the two reached 28% and 0.5%, respectively, and then the precipitate obtained by centrifugation was completely dissolved in distilled water, and the crude IgY aqueous solution was obtained after desalting by dialysis. Then add PEG6000 to the crude IgY aqueous solution and adjust the pH to 5.0. The final weight concentration of PEG6000 is 8%. Put it in the refrigerator for 1 hour and centrifuge to obtain the purified IgY precipitate. Finally, redissolve the purified IgY precipitate in distilled water and dialyze Remove PEG6000 to obtain purified IgY aqueous soluti...

Embodiment 2

[0029] (1) Take 500ml of egg yolk stock solution, add 3 times the volume of distilled water to dilute, adjust pH=4, stir evenly, put it in a refrigerator at 4°C overnight, rotate at 10000rpm, and centrifuge for 15min to obtain supernatant A and egg yolk pulp; then supernatant Ammonium sulfate and sodium chloride were added to liquid A to precipitate IgY, so that the final concentrations reached 20% and 1%, respectively, and then the precipitate obtained by centrifugation was completely dissolved in distilled water, and the crude IgY aqueous solution was obtained after dialysis to remove salt, and then the crude IgY aqueous solution was added to Add PEG6000 to the solution and adjust the pH to 7.0. The final concentration of PEG6000 is 4%. Put it in the refrigerator for 1 hour and centrifuge to obtain the purified IgY precipitate. Finally, add distilled water to dissolve the purified IgY precipitate again, and dialyze to remove PEG6000 to obtain the purified IgY. Aqueous solutio...

Embodiment 3

[0036](1) Take 500ml of egg yolk stock solution, add 12 times the volume of distilled water to dilute, adjust the pH to 7.0, stir evenly and let stand at 4°C overnight at a speed of 10000rpm, centrifuge for 15min to obtain supernatant A and egg yolk pulp; then add to supernatant A IgY was precipitated by ammonium sulfate and sodium chloride to reach a concentration of 35% and 0.1%, respectively, and then the precipitate obtained by centrifugation was completely dissolved in distilled water, and the crude IgY aqueous solution was obtained after dialysis to remove salt, and then PEG6000 was added to the crude IgY aqueous solution and Adjust pH=3.0, PEG concentration is 10%, put in the refrigerator for 1 hour and centrifuge to obtain the purified IgY precipitate, finally redissolve the purified IgY precipitate in distilled water, dialyze out PEG6000 to obtain the purified IgY aqueous solution, and freeze-dry IgY finished products can be obtained;

[0037] (2) Add 400ml of absolut...

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Abstract

The invention discloses a method for jointly extracting IgY, phosvitin, lecithin, egg oil and skim yolk powder from egg yolk. The method comprises the following steps: firstly, diluting yolk fluid by using water and centrifuging; salting out the acquired supernatant and performing polyethylene glycol sediment, thereby extracting IgY; for the yolk pulp on the bottom layer, removing water from the yolk pulp by adopting ethyl alcohol, extracting total yolk lipid by utilizing an ethyl alcohol and ethyl acetate mixed solvent and treating by utilizing ethyl acetate, thereby acquiring two products of lecithin and egg oil; treating the egg white by utilizing a sodium chloride solution after removing the lipid, thereby acquiring the phosvitin. According to the method, the technological operation is simple and convenient, environment-friendly and non-toxic effects are achieved, the production cost is low, the product purity and yield are higher and a technical support is supplied for the comprehensive utilization of yolk.

Description

technical field [0001] The invention belongs to the field of deep processing of egg products, in particular to a method for jointly extracting IgY, phosvitin, lecithin, egg oil and defatted egg yolk powder from egg yolk. Background technique [0002] Egg yolk is the most nutritious part of eggs. Its protein content is not only high, but also its amino acid composition is very similar to that of human protein. Lipids in egg yolks not only contain rich unsaturated fatty acids and suitable linoleic acid content, but also contain phospholipids that are indispensable for human beings in the brain and nervous system. Therefore, biological substances such as proteins and lipids in egg yolks The development of active ingredients has always been a research hotspot of scholars at home and abroad. [0003] Due to the complex composition of egg yolk, it is difficult to make the best use of it. At present, the research mainly focuses on the separation and extraction of single bioactive...

Claims

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Application Information

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IPC IPC(8): C07K16/02C07K14/465C07F9/10C11B1/10
CPCC07F9/103C07K14/465C07K16/02C11B1/10C11B1/108
Inventor 蔡朝霞罗进旭刘巧唐世涛付星马美湖
Owner HUAZHONG AGRI UNIV
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