Anticariogenic proteins and peptides and saccharides

Abstract

This Invention discloses new proteins & peptides & saccharides that haue anti cariogenic capabilities and that are characterized by the presence of one or more components that haue the ability to form a complex with calcium Ions: such as epsilon-polylysine that is conjugated with one or more bisphosphonyl- , biscarboxyl-, or 3-hydroxyphthalate-groups or conjugated with Casein phosphopeptide, phosvitin or with partially hydrolyzed phosvitin; such as partially hydrolyzed chitosan that is conjugated with one or more bisphosphonyl groups, Casein phosphopeptide or with phosvitin or partially hydrolyzed phosvitin; such as bisphosphonylated and biscarboxylated proteins with at least 40% of amino acids consisting of lysine and a molecular weight of above 2 kD (2000 dalton) and such as polymerized Casein phosphopeptide and partially hydrolyzed phosvitin. Especially basic polymers that are conjugated with bisphosphonyl-groups, such as bisphosphonylated epsilon-polylysine, are strong protectors due to the simultaneous presence in one molecule of strong calcium-complexing- and strong acid buffering components. In addition, polylysines, such as epsilon-polylysine haue demonstrated antibacterial activity against a large variety of oral cavity bacteria including acid producing bacteria. It indicates it's relevance for the protection of teeth in particular and for control of the bacterial flora in the oral cavity in general. The products can be used in formulations to protect teeth and to treat the oral cavity: toothpastes, gels, mouth rinses, artificial saliva's ....for patients and healthy consumers. They haue an attractive toxicological profile compared to fluoride, and can be used in food systems; they act additionally to the action of fluoride. The use in combination with fluoride provides excellent and enhanced protection at minimum fluoride dosage. The Invention encompasses competent protein & peptide & saccharide structures, based an in-vitro and in-vivo experiments.

Description

field of invention [0001] Caries is caused by oral bacteria that produce acids that dissolve tooth surfaces. [0002] The present invention relates to proteins, peptides and sugars capable of protecting teeth against acid attack. The present invention discloses the appropriate molecular structure required to produce the protective capacity, the production method of said protective agent and the development of the final formulation for tooth protection. Background of the invention [0003] It is well known that dental caries is caused by acids produced by bacteria in the mouth. Sugars such as glucose are an important food source for bacteria, which they use to produce acids. Streptococcus mutans (S.mutans) is considered to be the most important culprit in the occurrence of dental caries. The bacteria can survive well in the oral cavity and produce a considerable amount of acid. S. mutans also produces glucosyltransferases that catalyze the production of...

AI Technical Summary

Problems solved by technology

Although dextrose is used on a large scale in food, it needs to be replaced, and use of elevated doses may lead to undesirable diarrheal side effects

[0008] 3. It is possible to limit the adhesion of Streptococcus mutans to the tooth surface in various ways: pyrophosphate and ZnO can limit the formation of dental plaque and reduce the probability of adhesion (US 5455024); dextranase and dextranase can Degrades dextran used by bacteria to adhere to dental plaque (US 5468479); possible use of peptide vaccines that elicit an immune response against glucosyltransferases; this enzyme catalyzes the production of glucan (US 5686075); structure similar to Peptides from certain portions of Streptococcus mutans antigen I / II can compete with this antigen for adhesion to dental plaque (US 6500433)

However, it is resistant to enzymatic degradation and adheres to cell walls, making it irritating and toxic

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