48results about How to "Increased cleavage" patented technology

The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3′ end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.

The present invention relates to chirally controlled oligonucleotides of select designs, chirally controlled oligonucleotide compositions, and methods of making and using the same. In some embodiments, a provided chirally controlled oligonucleotide composition provides different cleavage patterns of a nucleic acid polymer than a reference oligonucleotide composition. In some embodiments, a provided chirally controlled oligonucleotide composition provides single site cleavage within a complementary sequence of a nucleic acid polymer.

The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3′ end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.

The present application describes a method for inducing or maintaining pluripotency in a cell by contacting the cell with a biological or chemical species that increases MUC1* activity.

Provided are methods for and compounds for modulating the complement system. In particular, compounds are provided that inhibit complement activation and compounds are provided that promote complement activation. The compounds are therapeutics by virtue of their effects on the complement system. Hence, the compounds that inhibit complement activation can be used for treatment of ischemic and reperfusion disorders, including myocardial infarction and stroke, sepsis, autoimmune diseases, inflammatory diseases and diseases with an inflammatory component, including Alzheimer's Disease and other neurodegenerative disorders.

Disclosed are methods of inactivating a protein, such as cleaving a disulfide bond of a protein, that involve contacting the protein with a reducing agent, a denaturant, and a hydroxide ion, wherein the pH of the composition is about 10.0 to about 14.0. Also disclosed are methods of treating or preventing a disease in a subject, such as a toxin-related disease or a prion-related disease, that involve contacting a subject with a pharmaceutically effective amount of a reducing agent, a denaturant, and a hydroxide ion, wherein the pH of the composition is about 9.0 to about 14.0. Also disclosed are compositions that include a reducing agent, a denaturant, and a hydroxide ion, wherein the pH of the composition is about 9.0 to about 14.0.

A multi-layer uplift bra which enhances a woman's natural curves while providing adequate support and coverage for the breasts includes a bottom portion capable of encircling the torso of a wearer, a pair of breast cups joined at a center portion, and a pair of straps, each of which being connected at one end to a breast cup and at another end to the bottom portion of the bra. The pair of straps are capable of extending over a pair of shoulders of the wearer. The breast cups include a first layer for lifting the breasts and creating cleavage and a second layer for supporting and holding the breasts in place. A joining means is provided for joining the first and second layers together along the bottom portion of the multi-layer uplift bra.

Kidney-shaped push up insert including first lobe and second lobe is provided. Notch is formed in top side between first lobe and second lobe. Insert has depression formed adjacent top side extending towards notch. Second main surface opposes first main surface. Second main surface is substantially smooth arcuate-shaped surface. First lobe has narrower width between top side and bottom side than second lobe, and second lobe is thicker between first main surface and second main surface than first lobe. Ratio of maximum thickness between first main surface and second main surface to maximum length between opposing ends of first lobe and second lobe is less than about 9 and / or ratio of depth of notch from a straight line connecting first lobe and second lobe along top side of insert to width of insert from top side to bottom side at depth of notch is less than about 4.

A soft stabproof bulletproof material is in a sandwich structure formed by intersecting and compounding a plurality of layers of fiber unidirectionally-orientated prepregs, wherein one of at least one bulletproof stabproof layer comprises three basic units, each basic unit is formed by orthogonally compounding high-strength high-modulus fiber unidirectionally-oriented prepregs, intersection angles 0-90 degrees of adjustment units are superposed and compounded, and organic films are adhered to two sides of each layer through block adhesive. The invention further discloses a method of preparing the soft stabproof bulletproof material. Favorable stab proofing of the soft stabproof bulletproof material is maintained while bullet proofing of the soft stabproof bulletproof material is improved. The soft stabproof bulletproof material can be used independently or used with other materials to apply to making of multi-specification bulletproof stabproof garments. The soft stabproof bulletproof material light in weight and fine in defensive performance can be continuously rolled on a large scale.

The present invention provides methods of diagnosis, providing a prognosis and a therapeutic target for the treatment of cancers according to their expression of N-cadherin, including prostrate and bladder cancers.

The invention relates to improvement of an inverted umbrella shaped aerator and is characterized in that the inverted umbrella conical surface is cut into multiple slices, and the slices are distorted for an angle of 5-15 degrees into water in the same direction; the slices are distributed and still in the shape of an inverted umbrella, the function that the inverted umbrella shaped aerator generates negative pressure through rotational flow to carry air so as to achieve aeration and oxygenation is maintained, the inclined slices with small angles have double-flow function of horizontal plug flow and downward plug flow, thereby not only meeting the requirement of Carrousel oxidization ditch plug flow, but also replacing omitted plug flow impellers. Compared with the prior art, the inverted umbrella shaped aerator achieves triple effects of strengthening aerator effect, saving raw materials and reducing energy consumption, and has the advantages that oxygenated bubbles are finer, and dynamic efficiency can be improved by about 10%-12%, oxygenation capability can be improved by 15%-25%, and oxygenation water depth can be increased by 30%.

The invention relates to a high-lift helical-blade impeller which comprises a pump shaft sleeve, an impeller ring and a blade. The impeller ring is arranged at the end portion of the pump shaft sleeve, and the impeller ring is provided with the blade; the blade is in a helical shape; the spiral wrap angle of the blade is 200-220 degrees; the entrance installation angel of the blade is 10-12 degrees; the exit installation angel of the blade is 14-18 degrees; the impeller ring is of a cone shape; the cone angle of the impeller ring is 6-10 degrees; the width of the impeller ring is 40-52 mm; and the impeller ring is uniformly provided with a plurality of keyholes. The high-lift helical-blade impeller improves delivery capacity of a pump in transmitting a high gas content medium.

The present invention relates to enantiomeric forms of hepoxilin analogs of Formula I-VIII, pharmaceutical compositions thereof, a method for the separation of said enantiomeric forms of hepoxilin analogs comprising applying said hepoxilin to a chiral phase HPLC column and eluting said hepoxilin with an alkane and alcohol solvent mixture. Said enantiomeric forms of hepoxilin analogs of Formula I-VIII were found to be useful in controlling the biological effects of PPAR mediated transcriptional control for the treatment of diseases such as cancer, thromboxane-mediated diseases and for modulating intracellular calcium concentration.

A multi-layer uplift bra which enhances a woman's natural curves while providing adequate support and coverage for the breasts includes a bottom portion capable of encircling the torso of a wearer, a pair of breast cups joined at a center portion, and a pair of straps, each of which being connected at one end to a breast cup and at another end to the bottom portion of the bra. The pair of straps are capable of extending over a pair of shoulders of the wearer. The breast cups include a first layer for lifting the breasts and creating cleavage and a second layer for supporting and holding the breasts in place. A joining means is provided for joining the first and second layers together along the bottom portion of the multi-layer uplift bra.

The present invention relates to methods to treat a neurodegenerative disease or disorder, e.g., a motor neuron disease in a subject, whereby the subject is administered a recombinant human Mullerian Inhibiting Substance (MIS) protein as disclosed herein, wherein the recombinant human MIS protein comprises a modified Kex cleavage site for increased cleavage. The recombinant human MIS protein can be produced from a pre-proprotein comprising a non-MIS leader sequence or a functional fragment thereof in place of the MIS leader sequence.

The invention discloses an aromatic planar Schiff-base copper complex. The chemical formula of the aromatic planar Schiff-base copper complex is [CuL(H2O)](ClO4)-H2O, wherein L is 8-aminoquinoline salicylidene Schiff-base, and ClO4 is perchlorate. The preparation method of the aromatic planar Schiff-base copper complex comprises synthesis of a ligand L and synthesis of an aromatic planar Schiff-base copper complex. The synthetic process is simple and has good selectivity and stability. There is intercalation of intermediate bonding between the aromatic planar Schiff-base copper complex and CT-DNA. Under the close physiological condition of existence of a redox inducer, it shows an obvious cutting effect on Pbr322 DNA; and ultraviolet light has a certain promotion effect on the cutting effect. The Pbr322 DNA can be fractured through an auto-oxidation approach, and a hydroxyl radical active matter is generated in the cutting process.

The invention includes a genetic construct and a mutant dengue virus, designated as strain MBU 01-2002, which is a mutant dengue virus generated by genetic modification of the 13-amino acid sequence just proximal to the pr-M junction within the prM coding region of the genome. The modification involves increasing the number of positively charged amino acid and abolishing the negatively charged amino acid in this pr-M junction sequence. The mutant dengue virus strain MBU 01-2002 possesses less prM protein on the viral envelope than the prototype dengue virus, is capable of inducing infected C6 / 36 cells to fuse at neutral pH, is as efficient as the prototype virus in the intracellular multiplication, but is defective in its release from infected cells.

The invention relates to micro-bubble purifying apparatus and a micro-bubble purifying method. When gas enters the micro-bubble purifying apparatus, the gas is uniformly distributed under the action of a first-layer gas distributor, and enters a purifying chamber through holes of the first-layer gas distributor so as to form relatively tiny liquid bubbles; and then, the liquid bubbles ascend so asto capture most of dust due to contact of the dust with liquid membranes of liquid bubble walls of the liquid bubbles. When the gas passes through a second-layer gas distributor, speed of the liquidbubbles passing through plate holes increases, so that cutting effects on the liquid bubbles are enhanced so as to further break the liquid bubbles to form dust-capturing micro-bubbles; at the same time, cavitation effects and mechanical actions of ultrasound are utilized when cutting is performed on the liquid bubbles by the second-layer gas distributor, so that high-frequency vibration acts on surface walls of the micro-bubbles to break part of the micro-bubbles further; and thus, contact rate of the liquid membranes with particulates is increased due to vibration of surfaces of part of themicro-bubbles, so that contact frequency of smoke with the liquid bubble walls is improved so as to enhance gas-liquid mixing effects. Finally, fine dust in the smoke fully contacts with purifying liquid bubble walls so as to reduce escape probability, so that purification effects on fine dust is achieved.

The present invention relates to compounds with activity as inhibitors of sAPPβ and Aβ production, and methods for treating, preventing, or ameliorating neurodegenerative diseases, such as Alzheimer's disease and pharmaceutical compositions containing such candidate compounds.

The invention provides a high-COD and high-ammonia nitrogen characteristic sewage bioreactor. The high-COD and high-ammonia nitrogen characteristic sewage bioreactor comprises a sewage treatment pool;the sewage treatment pool is characterized by being provided with a high-concentration active sludge layer S1, a medium-concentration sludge layer S2, a movable fluidization denitrifying layer S3, asolid and liquid separation layer S4 and a clarifying layer S5 sequentially from bottom to top according to the order of the oxygen content of wastewater from high to low; a carrier suitable for survival of biological denitrification bacteria is arranged in the movable fluidization denitrifying layer S3; and an aeration device is arranged at the lower end of the high-concentration active sludge layer. The reactor integrates an active sludge method, a biological membrane method and a settling pool (a clarifying pool), realizes aerobiotic, anoxic and anaerobic areas through dissolved oxygen decline curve principle and obstruction of the carrier biological membrane and is different from the pool separation arrangement of the traditional A / O process, aerobes and facultative microorganisms coexist in the depth direction of the sewage treatment pool, good stereoscopic functional partition is realized, the COD removal rate reaches to more than 90 percent, the NH3-N removal rate is more than 90 percent, and the system achieves a good COD and ammonia nitrogen removing effect.

The present invention provides a method for cleaving a glycated protein to obtain an amino acid or a peptide efficiently with a protease. By treating the glycated protein with the protease in the presence of a compound represented by R—X, the amino acid or the peptide is obtained by the cleavage. The R represents an alkyl compound with a carbon number of 9 or more, and preferably is straight-chain alkyl or straight-chain acyl with a carbon number of 9 to 16, branched-chain alkyl or branched-chain acyl with a carbon number of 10 to 40 and a main-chain carbon number of 9 to 16, or straight-chain alkyl that is substituted by cycloalkyl (a carbon number of the cycloalkyl ranges from 3 to 8, and a carbon number of the straight chain ranges from 4 to 13), where X is a sugar residue. Moreover, the glycated protein is, for example, glycated hemoglobin, and preferably β-chain N-terminal amino acid or a β-chain N-terminal peptide is cleaved by the protease treatment.

Methods and compositions are provided for improving specificity during amplification of a target DNA sequence. The methods and compositions rely upon the use of an RNase H enzyme, a polymerase, and RNase H enzyme-sensitive, blocked-cleavable oligonucleotide primers in the amplification reactions, wherein the reaction mixtures include either an optimized final concentration of a divalent metal salt comprising 2.0 mM or less of free Mg++ cation and / or an optimized final concentration of a non-ionic detergent comprising at least about 0.001% polyethylene glycol hexadecyl ether.

Disclosed is a process for producing an ortho-metalated 1:3 complex of iridium with homoligands which is useful as a luminous material for an organic EL device in high yield at high purity in a short time by a simple procedure. An example of the complex is tris(2-phenylpyridine)iridium(III) and this complex can be produced by reacting iridium(III) acetylacetonate with an organic ligand consisting of 2-phenylpyridine in the copresence of an acidic substance, either an organic acid such as tartaric acid or an inorganic acid such as phosphoric acid.

A bra including two fabric cups joined at the center front of the bra, each cup having an upper portion and a lower portion. The lower portion of each cup is constructed to generally follow the inframammary crease of a respective woman's breast. The top of the upper portion of each cup includes a dart. A generally U-shaped support piece composed of an elongated strip of elastic is attached to the bra along the bottoms and sides of the lower portion of the cups. The support piece is made of fabric which is less elastic than the fabric of the cups. Bands made of low-stretch material extend from the sides of the lower portion of the cups and include generally V-shaped inserts cut on the bias in the tops of the bands. The inserts are made of fabric which is more elastic than the fabric of the bands.

We disclose a method of diagnosis of a disease, or susceptibility to a disease associated with abnormal cell-cell adhesion between epithelial cells, the method comprising detection of a mutation in a nucleic acid encoding an adhesion protein, a protease, or a protease inhibitor of an individual. Methods of making medicaments and methods of treatment and / or prophylaxis are also disclosed.