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Application of phosvitin

A phosvitin and application technology, which is applied in the protein field, can solve the problems of reducing Helicobacter pylori and not finding the inhibitory effect of phosvitin, and achieve the effect of improving the application value

Pending Publication Date: 2021-11-23
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, Luo Qiang and others studied the inhibitory effect of active substances (such as various aldehyde compounds) in Maotai-flavored liquor on Helicobacter pylori, and the results showed that these biologically active substances in Maotai-flavored liquor can Reduce the adhesion of Helicobacter pylori to related cells, and at the same time inhibit the expression of related virulence genes of Helicobacter pylori, thereby helping to inhibit the damage of Helicobacter pylori to the human gastrointestinal mucosa. In addition to the above research results, More and more studies are involved in demonstrating the inhibitory effect of some food-borne components on H. pylori, but the inhibitory effect of phosvitin on H. pylori has not been found

Method used

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  • Application of phosvitin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A process for extracting phosvitin, comprising the following steps:

[0018] (1) Break the eggshells of 24 eggs, separate the egg whites with an egg yolk separator, roll the egg yolks on filter paper, remove the egg whites and egg bands, cut the egg yolk membrane with a toothpick, collect the egg yolk liquid, and keep the egg yolk liquid at 1 ~5°C;

[0019] (2) Add 1L of water to the egg yolk liquid, adjust the pH to 5, stir at 4°C for 6h, then centrifuge at 7000rmp for 1h to collect the precipitate, then add 800mL of 0.05mol NaCl solution, and stir 4h. Then centrifuge at 7000rmp15°C for 30min to collect the precipitate. Put the collected precipitate in a large beaker, add 600ml of n-hexane and 200mL of ethanol organic mixed solution to mix, stir in a fume hood at 4°C for 6h, suction filter into a cake shape after stirring, and then add 400mL containing A solution of 1.74mol NaCl was stirred at 4°C to obtain the first liquid;

[0020] (3) Centrifuge the first liquid...

Embodiment 2

[0022] An iron chelation treatment method for phosvitin, comprising the following steps:

[0023] a1. Prepare 10 mL of ferrous ammonium sulfate solution of 0.15 mol / L for use, then take 0.4 g of phosvitin and dissolve it completely in 40 ml of water to obtain 10 mg / mL of phosvitin solution, and then adjust the pH to 6.5;

[0024] a2. Take 5mL 0.15mol / L ferrous ammonium sulfate solution and add it to 10mg / mL 40mL phosvitin solution, stir well and mix thoroughly for 1 hour, then centrifuge in a centrifuge for 15 minutes after mixing, and obtain the supernatant after centrifugation Put it into a dialysis bag, and dialyze for 48 hours. After the dialysis is completed, pour the dialyzed liquid into a plate, freeze and form it at -80°C, and then freeze-dry it in a freeze dryer to obtain the final iron-chelated phosvitin ;

[0025] a3. Preserving the obtained iron-chelated phosvitin at 4°C;

Embodiment 3

[0027] A method for dephosphorylation of phosvitin, comprising the following steps:

[0028] b1. First prepare 20ml of 0.4mol / L NaOH solution, then mix 0.5g phosvitin with the prepared NaOH solution, pour the mixed liquid into a 50ml centrifuge tube and heat in a water bath at 37°C for 3 hours, Then adjust the pH to 7 with 1mol / L HCL to obtain a mixed solution;

[0029] b2. Pour the mixed solution into a dialysis bag and dialyze in a beaker filled with pure water for 48 hours. Change the dialysis water regularly. After the dialysis is completed, pour it into a plate, freeze it in a refrigerator at -80°C, and finally place it in a freeze dryer. Lyophilized to dephosphorylated phosvitin;

[0030] b3. Store the obtained dephosphorylated phosvitin at 4°C.

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Abstract

The invention provides an application of phosvitin to inhibition of helicobacter pylori. According to the application of the phosvitin, the antibacterial potential of the phosvitin which is a functional food-borne substance is excavated, and the application value of the phosvitin is further improved.

Description

technical field [0001] The invention relates to the technical field of proteins, in particular to the use of phosvitin. Background technique [0002] The treatment of Helicobacter pylori has always been concerned. In the early stage of clinical treatment, "triple therapy" was often used as the main program for eradicating Helicobacter pylori. The drug formulations included PPI, clarithromycin, and amoxicillin. However, Helicobacter pylori has shown high drug resistance to several drugs involved in this therapy. It is currently replaced by "quadruple therapy" with a higher eradication rate, but the consequence is that the treatment period is longer and the cost of treatment is higher. At the same time, antibiotic drug treatment will also bring some side effects, such as the disturbance of intestinal flora, and the increase of bacterial resistance, which will make it more difficult to treat H. pylori infection. Therefore, a more sensible choice is to look for natural active ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K38/40A61K47/22A61K47/26A61K9/12A61P31/04A61P1/00A61P31/14
CPCA61K38/1703A61K38/40A61K47/22A61K47/26A61K9/0014A61K9/006A61P31/04A61P1/00A61P31/14A61K9/12A61K2300/00
Inventor 段翔张晶昊贾劼田亮杰兰莹刘学波
Owner NORTHWEST A & F UNIV
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