33 results about "Bostrichthys sinensis" patented technology

The invention relates to an indoor bostrichthys sinensis fry intermediate rearing method, and relates to fish fry rearing. According to the invention, pipes and air pumps are placed in a plastic tank; the plastic tank is filled with seawater; bleaching powder is added for disinfection, and the plastic tank is washed by using seawater; a sun shade net is washed by using seawater, and is exposed under sunlight; living water salinity of the fries before capturing is determined; if the living water salinity is different by no more than 10 than the seawater salinity in the plastic tank, the fries are directly placed in the plastic tank for intermediate rearing; if the salinity difference is higher than 10, the fries are subjected to 1-2 days of transition, and are transferred into the plastic tank for intermediate rearing; the fries are not fed the day the fries are placed into the plastic tank; after that, the fries are fed with bait-feed once every morning and evening; fry rearing water is replaced every two days; light intensity is kept below 6lux and seawater dissolved oxygen is maintained at (5.84+ / -0.184)mg L<-1>. When fries achieve full lengths of 8.2cm and weights of 8.0g with intermediate rearing, the fries are transferred into a culturing pond and are subjected to adult fish culturing.

The invention discloses a marker and method for identifying single nucleotide polymorphism of a bostrichthys sinensis group and relates to a method for identifying a bostrichthys sinensis group. A single nucleotide polymorphism marker of the bostrichthys sinensis group is positioned on a 29th basic group at a 5' end of a praline tRNA gene in a bostrichthys sinensis mitochondrial genome. The method for identifying single nucleotide polymorphism of the bostrichthys sinensis group comprises the steps of: extracting a genome DNA of a bostrichthys sinensis; carrying out PCR (Polymerase Chain Reaction) amplification on the praline tRNA gene in the bostrichthys sinensis mitochondrial genome by using the DNA as a template, carrying out electrophoretic analysis on the PCR amplification products, determining that the sample is from the bostrichthys sinensis group in North of Pearl River Estuary when 330-bp PCR amplification products are tested, and determining that the sample is from the bostrichthys sinensis group in South of Pearl River Estuary when 330-bp PCR amplification products are not tested. The method for identifying single nucleotide polymorphism of the bostrichthys sinensis group is simple and easy, accurate and fast, impartial and subjective as well as economical and practical.

The invention discloses a method suitable for live transport of Channa sinensis, relating to a live transport method for aquatic animals. Catch S. sinensis from the breeding pond and temporarily raise it in a cement pond. During the temporary cultivation period, the pool water is oxygenated and no feeding is made to empty the food in the digestive tract of S. sinensis. When transporting in summer and autumn, gradually reduce the water temperature. During transportation in winter and spring , no need to lower the water temperature; the transport device adopts a box body, and the box body is equipped with ice cube placement holes, and ice cubes can be placed as needed; it is transported by means of transportation, and the wet wipes are covered on the surface of the snakehead snakehead during transportation to keep the fish body Keep the skin moist and maintain a suitable temperature in the box; after arriving at the destination, unload and open the box, move the Chinese snakehead into low-salinity seawater to resuscitate, and adjust the temperature of the resuscitated seawater to be similar to the temperature in the box. It has the advantages of large transport quantity per unit volume, low transport cost, simple operation and high transport efficiency. It can maintain a high survival rate during long-distance transportation.

The invention discloses a micro-granular feed for larva fish of bostrichthys sinensis. The micro-granular feed comprises the following components in parts by weight: 50-70 parts of animal nutritional ingredients, 15-25 parts of plant nutritional ingredients, 10-25 parts of functional ingredients, 0.5-2 parts of special vitamins, 0.5-2 parts of special trace elements, 0.5-1.5 parts of casein hydrolysate and 0.5-1.5 parts of lysine. A preparation method comprises the following steps: (1) manufacturing raw materials; (2) synthesizing a biological material; (3) converting to a cooked material; (4) puffing and granulating; (5) drying; (6) spraying and coating; and (7) performing vacuum drying. The feed disclosed by the invention adopts scientific matching and an advanced processing technology is used for assistance, so that the digestion and absorption rate of the feed is effectively improved, the growth of the larva fish of bostrichthys sinensis can be effectively promoted, and the occurrence of diseases during cultivation is reduced.

The invention relates to the technical field of fluorescent quantitative PCR detection methods, in particular to a primer and a real-time fluorescent quantitative PCR method for detecting the Mn-SOD gene of Chinese black snakehead (Bostrychus sinensis), which uses real-time fluorescent quantitative PCR technology to analyze Chinese black snakehead The expression of Mn‑SOD gene in different tissues of Channa sinensis, using GAPDH as an internal reference gene, designing internal reference gene primers and target gene primers; using reverse transcribed cDNA as a template, the expression of the target gene on the ABI 7500 Fast Real‑time PCR system It can quickly and accurately detect the expression and distribution of Mn-SOD gene in different tissues of Channa sinensis, and provide a scientific basis for the utilization and research of this gene.