33 results about "Bostrichthys sinensis" patented technology

The invention discloses a marker and method for identifying single nucleotide polymorphism of a bostrichthys sinensis group and relates to a method for identifying a bostrichthys sinensis group. A single nucleotide polymorphism marker of the bostrichthys sinensis group is positioned on a 29th basic group at a 5' end of a praline tRNA gene in a bostrichthys sinensis mitochondrial genome. The method for identifying single nucleotide polymorphism of the bostrichthys sinensis group comprises the steps of: extracting a genome DNA of a bostrichthys sinensis; carrying out PCR (Polymerase Chain Reaction) amplification on the praline tRNA gene in the bostrichthys sinensis mitochondrial genome by using the DNA as a template, carrying out electrophoretic analysis on the PCR amplification products, determining that the sample is from the bostrichthys sinensis group in North of Pearl River Estuary when 330-bp PCR amplification products are tested, and determining that the sample is from the bostrichthys sinensis group in South of Pearl River Estuary when 330-bp PCR amplification products are not tested. The method for identifying single nucleotide polymorphism of the bostrichthys sinensis group is simple and easy, accurate and fast, impartial and subjective as well as economical and practical.

The invention discloses an artificial breeding method of Bostrichthys sinensis. Currently, only natural seedlings are used in the breeding of the Bostrichthys sinensis, and small-scale artificial breeding is successful, but has the defect that limited seedlings can be provided. In order to solve the problem of shortage of Bostrichthys sinensis seedlings, technical measures including parent fish selection, induced spawning, artificial incubation and fish fry breeding are adopted in the artificial breeding method, and a large-scale artificial breeding technology of the Bostrichthys sinensis seedlings is searched. The artificial breeding method is scientific and reasonable in process flow and strong in operability. The large-scale artificial breeding of the Bostrichthys sinensis is realized, and weighted according to fertilized eggs, the breeding survival rate is up to over 58.5%. A technical support is provided for the large-scale industrial breeding of the Bostrichthys sinensis seedlings, and the defects of low technological content and small breeding amount of the conventional breeding method are overcome.

The invention discloses a granular feed for juvenile fish of bostrichthys sinensis. The granular feed comprises the following components in parts by weight: 40-60 parts of animal nutritional ingredients, 20-40 parts of plant nutritional ingredients, 15-30 parts of functional ingredients, 0.3-3 parts of special vitamins, 0.3-3 parts of special trace elements, 0.2-2 parts of casein hydrolysate and 0.2-2 parts of lysine. A preparation method comprises the following steps: (1) manufacturing raw materials; (2) synthesizing a biological material; (3) converting to a cooked material; (4) puffing and granulating; (5) drying; (6) spraying and coating; and (7) performing vacuum drying. The feed disclosed by the invention adopts scientific matching and an advanced processing technology is used for assistance, so that the digestion and absorption rate of the feed is effectively improved, the growth of the juvenile fish of bostrichthys sinensis can be effectively promoted, and the occurrence of diseases during cultivation is reduced.

The invention discloses a method for 3D culturing testis cells of bostrychus sinensis to generate sperms. The method for 3D culturing the testis cells of the bostrychus sinensis to generate the spermscomprises the following steps that testes of bostrychus sinensis milters are taken out, cleaned, sterilized and cut into pieces, testis fragments are placed in digestive juice, digestion is carried out on a constant-temperature horizontal shaking table, and a testis cell suspension is prepared; incompletely digestive tissues or large cell clusters are removed through cell mesh screen filtering, filtered cells are subjected to low-speed centrifugation through Percoll gradient, and the testis cells of the bostrychus sinensis after low-speed centrifugal purification are collected; bostrychus sinensis sperm culture mediums and protein additives are added for resuspension, and inoculation is carried out in a 3D cell culture dish for culturing; in the process of 3D culturing, immunofluorescenceis used for detecting germ cells; one half of fresh sperm culture mediums are replaced every 2-5 days, the number of the sperms is counted, and eosin Y dyeing detects the survival rate of the cultured sperms; and the bostrychus sinensis sperms generated by 3D culturing and ripe eggs of the bostrychus sinensis are subjected to in vitro fertilization, and the fertilization rate of an embryo is counted.

The invention discloses a micro-granular feed for larva fish of bostrichthys sinensis. The micro-granular feed comprises the following components in parts by weight: 50-70 parts of animal nutritional ingredients, 15-25 parts of plant nutritional ingredients, 10-25 parts of functional ingredients, 0.5-2 parts of special vitamins, 0.5-2 parts of special trace elements, 0.5-1.5 parts of casein hydrolysate and 0.5-1.5 parts of lysine. A preparation method comprises the following steps: (1) manufacturing raw materials; (2) synthesizing a biological material; (3) converting to a cooked material; (4) puffing and granulating; (5) drying; (6) spraying and coating; and (7) performing vacuum drying. The feed disclosed by the invention adopts scientific matching and an advanced processing technology is used for assistance, so that the digestion and absorption rate of the feed is effectively improved, the growth of the larva fish of bostrichthys sinensis can be effectively promoted, and the occurrence of diseases during cultivation is reduced.

The invention discloses a bostrychus sinensis feeding method and an acoustic domestication feeding system thereof. The method is characterized in that excellent diatom is inoculated after water changing, chaetoceros is selected as the diatom, the feed feeding is decreased progressively according to the levels of 8%, 7%, 6%, 5% and 4% of the total weight of fish, each level is fed for 3 days, 15 days serve as a period, after 15 days, 100% water change is carried out, a fishing pond is cleaned, weighing is carried out, the above operation is repeated, an acoustic domestication feeding system isadopted for feeding during feeding, the acoustic domestication feeding system comprises a support frame, and a hydrophone and a camera which are positioned in a culture pond, four feeding machines, aloudspeaker and a controller are arranged on the support frame, and the feeding machines, the loudspeaker, the hydrophone and the camera are all electrically connected with the controller. The methodhas the functions of improving the feeding efficiency, reducing the feed loss, adjusting the pH value of the water body and inhibiting the growth of bacteria, so that the fatality rate of the bostrychus sinensis is reduced, and the survival rate of the bostrychus sinensis is increased.

The invention discloses an artificial selecting-breeding method of antibacterial-disease good varieties of bostrichthys sinensis. The artificial selecting-breeding method includes the specific steps that survival fish fingerling entities after bacterial diseases are collected in high disease-affecting season; chorionic gonadotropin and luteinizing hormone-releasing hormone are injected into female fishes before parent fishes lay eggs, and chorionic gonadotropin is injected into male fishes; fertilized eggs are incubated at the temperature of 20 DEG C to 30 DEG C, and the antibacterial-disease good varieties of the bostrichthys sinensis are obtained. The artificial selecting-breeding method has the advantages that the survival fish fingerling entities after bacterial diseases in high disease-affecting season are selected as the parent fishes, and the antibacterial-disease capacity is high; the problem that fry of the bostrichthys sinensis are short is solved, large-scale breeding of the fry of the bostrichthys sinensis is achieved, the requirements of farmers for artificial fry of the bostrichthys sinensis are met, and the artificial selecting-breeding method is suitable for application and popularization of bostrichthys-sinensis breeding farms in national coastal areas, and has the large development market space.

The invention belongs to the technical field of biology, and particularly relates to a sperm cryopreservation liquid and application in sperm cryopreservation of bostrichthys sinensis. Thesperm cryopreservation liquid comprises asperm diluent and an anti-freezing protective agent, wherein the sperm diluent comprises sodium chloride, sodium dihydrogen phosphate, sodium bicarbonate, potassium chloride, calcium chloride dihydrate, D-glucose and magnesium sulfate heptahydrate, and the anti-freezing protective agent comprises dimethyl sulfoxide, bovine serum albumin and trehalose, and the sperm cryopreservation liquid is used for cryopreserving bostrichthys sinensis sperms and is small in damage. The invention further provides a cryopreservation method of bostrichthys sinensis sperms, wherein the method is simple, convenient and rapid, is used for cryopreservation of the bostrichthys sinensis sperms, is high in sperm motility after recovery and high in in-vitro fertilization rate, and can be used for protection of bostrichthys sinensis germplasm resources and large-scale artificial propagation and production.

The invention discloses a rapid cultivation feed for bostrichthys sinensis. The rapid cultivation feed comprises the following components in parts by weight: 50-65 parts of animal nutritional ingredients, 20-35 parts of plant nutritional ingredients, 5-15 parts of functional ingredients, 0.2-2 parts of special vitamins, 0.2-2 parts of special trace elements, 0.1-1 part of casein hydrolysate and 0.1-1 parts of lysine. A preparation method comprises the following steps: (1) manufacturing raw materials; (2) synthesizing a biological material; (3) converting to a cooked material; (4) puffing and granulating; (5) drying; (6) spraying and coating; and (7) performing vacuum drying. The feed disclosed by the invention adopts scientific matching and an advanced processing technology is used for assistance, so that the digestion and absorption rate of the feed is effectively improved, the rapid growth of bostrichthys sinensis can be effectively promoted, and the occurrence of diseases during cultivation is reduced.

The invention belongs to the field of aquaculture and discloses a method for adopting an iron cage for breeding bostrichthys sinensis under a mangrove forest. According to the method, the periphery and the bottom of the iron cage are all made of iron nets, the top of the iron cage is made of a solid iron plate, baffles are arranged at the lower ends of the periphery of the iron cage, and the height H of the iron cage is greater than the height h of each baffle, wherein the height h of each baffle is 10-30 cm. The method includes the steps of pushing the iron cage into silt under the mangrove forest, wherein the downward pushing depth of the iron cage is greater than the height h of each baffle and smaller than the height H of the iron cage, and during flood tide, seawater can at least inundate the area under the mangrove forest; then, putting bostrichthys sinensis into the iron cage for breeding. By adopting the provided method for breeding bostrichthys sinensis, the usage of baits canbe reduced, and the survival rate is increased. Additionally, since the provided method belongs to ecological breeding simulation, the taste and nutrient value of obtained bostrichthys sinensis are both identical to those of wild bostrichthys sinensis.

The invention relates to a marine ecological system with mangrove forest planting coupled with bostrichthys sinensis culture. The system comprises a mangrove forest planting area, a bostrichthys sinensis culture area and a migrant bird foraging area in a coast intertidal zone, and the area ratio of the mangrove forest planting area, the bostrichthys sinensis culture area and the migrant bird foraging area is equal to the golden section ratio of 1: 1: 1. First and second planting areas and first and second wall bodies are oppositely arranged; a groove is formed between the first wall body and the second wall body, a net body is fixed on the inner wall of the groove, and the net body is higher than the top surfaces of the wall bodies and exceeds the highest tide water level of the place; and when the tide rises, the bostrichthys sinensis is enclosed in the net body, and when the tide falls, the wall bodies and a gate retain part of seawater for survival of the bostrichthys sinensis. The system is beneficial to healthy growth of mangrove forests and water purification, an approximate natural growth environment of the bostrichthys sinensis is built, a large amount of substances and energy are provided for the bostrichthys sinensis, the quality and flavor of the bostrichthys sinensis are improved, meanwhile, foraging and inhabiting of migrant birds are guaranteed, and the system has important economic benefits, ecological benefits and social benefits.

The invention provides a bait additive for preventing parasitic caligus of bostrichthys sinensis cultured in a seawater pond. A first kind of additive consists of garlic paste accounting for 1 to 3 percent of the bait weight and a multi-vitamin mixture accounting for 2 to 4 percent of the bait weight; and a second kind of additive consists of garlicin accounting for 0.2 to 0.6 percent of the bait weight and a multi-vitamin mixture accounting for 2 to 4 percent of the bait weight. Sharp taste of the two additives can prevent the caligus from adhering in oral cavities of the bostrichthys sinensis and can also repel the caligus which adheres inside the oral cavity but is not eaten by the sick fish, so that an anti-stress capacity of the sick fish can be enhanced, and the inflammation diminishing and healing of wound of the oral cavity after the caligus is split can be promoted. The bait additive also has an action on improving the appetite of the bostrichthys sinensis.

The invention provides a method for detecting estrogen pollutions in a water body based on recombined Bostrychus sinensis Vitellongenin and an application, relates to the field of environmental pollution detection and immunology, and discloses a method for preparing a polyclonal antibody by taking recombinant protein as an antigen based on the recombined Bostrychus sinensis Vitellongenin VTG. A fast and accurate immunoassay method for detecting the VTG is established. By means of the method, male Medaka induced by 17-beta estradiol (E2) is detected, the VTG level of male fish induced by E2 isfound to be significantly higher than that of non-induced male fish, and the method is used to identify female fish and male fish and the VTG in the female fish is found to be obviously higher than that of the male fish. The difference is extremely significant, and the technology can be used to monitor the environmental estrogen pollutions and identify the male fish and the female fish.

A recombinant expression and purification method of vitellogenin from bostrichthys sinensis belongs to the technical field of fusion protein expression and purification method. The method of the invention comprises the following steps: providing a vitellogenin gene based on bostrichthys sinensis, cloning part of the gene, recombining plasmids in vitro, transferring to escherichia coli for prokaryote expression, obtaining high-efficiency fusion expression at 16 DEG C of IPTG induction temperature, wherein the expressed target protein exists in a soluble state and accounts for more than 25% of total of bacterial soluble protein; applying a purification technology to obtain a fusion protein MNP-VTG, and separating a label MNP from the fusion protein to obtain a target protein VTG monomer through sucrose digestion. The method of the invention has high-efficiency expression, simple purification of VTG, more simple separation than inclusion bodies due to solubility and no need for renaturation. The obtained recombinant VTG provides stable and uniform raw material basis for subsequent applications. The recombinant protein can be used for biological activity function researches, and made into corresponding antibodies for detection research.

The invention relates to a method for transporting live aquatic animals, in particular to a method suitable for transporting live bostrichthys sinensis. According to the method, bostrichthys sinensis is caught from a culturing pond and temporarily cultured in a cement pond, water in the pond is oxygenated in the period of temporary culturing, feeding is not performed, and therefore food in the digestive tracks of the bostrichthys sinensis is emptied; in the transportation process in summer and autumn, the temperature of the water is gradually lowered, and in the transportation process in winter and spring, the temperature of the water does not need to be lowered; boxes are adopted as transportation devices, ice block placing holes are formed in the boxes, and ice blocks are placed according to needs; transportation is performed through a traffic tool, and the surfaces of the bostrichthys sinensis are covered with wet clothes in the transportation process, so that the skins of the bostrichthys sinensis bodies are kept wet, and the appropriate temperature of the boxes is kept; after arriving at a destination, the boxes are detached and opened, the bostrichthys sinensis is moved into low-salinity sea water for revival, and the temperature of the sea water for revival is adjusted to be close to the temperature inside the boxes. The method has the advantages that the transportation quantity per unit volume is large, transportation cost is low, operation is easy, and transportation efficiency is high. A high survival rate in the long-distance transportation process can be kept.

The invention provides a bostrichthys sinensis feed containing Chinese herbal medicine components. The feed comprises the following components in parts by weight: 18-27 parts of fish meal, 14-18 parts of bean pulp, 3-7 parts of cottonseed meal, 17-24 parts of wheat bran, 15-28 parts of corn flour, 2-5 parts of rosemary powder, 2-6 parts of beer yeast, 2-3 parts of radix scutellariae, 1-2 parts of radix isatidis, 2-4 parts of rhizoma dioscoreae, 1-3 parts of radix geutianae, and 0.2-0.5 part of phagostimulant. A preparation method of the bostrichthys sinensis feed containing the Chinese herbal medicine components provided by the invention is simple; some Chinese herbal medicines are added, so the physiological function of bostrichthys sinensis is improved whilst the bostrichthys sinensis takes nourishment through the feed, the immunity is boosted, and the digestion is promoted.

The invention discloses a method for producing functional sperms by 3D in-vitro culture of bostrichthys sinensis spermatogonium, which comprises the following steps: (1) disinfecting, cleaning and cutting separated bostrichthys sinensis testis, adding a testis digestive juice for digestion, and filtering by a filter screen to remove undigested cell debris or cell clusters to prepare a testis single-cell suspension; adding the testis single-cell suspension into a percoll gradient solution for centrifugation, and separating to obtain spermatogonium; the percoll gradient solution is composed of a percoll solution with two concentrations of 22%-30% and 35%-45%; (2) transferring the spermatogonium into a 3D culture dish, and culturing the spermatogonium with a sperm induction culture medium; the sperm induction culture medium comprises a basic sperm culture medium and sex hormone, and further comprises melatonin. According to the method, the separation, culture and induction conditions of the spermatogonium are optimized and improved, and the efficiency of generating functional sperms by culturing the spermatogonium in vitro is improved.

The invention relates to the field of aquaculture and discloses a method of using an iron cage for cultivating bostrichthys sinensis on a beach. The circumference and bottom of an iron cage body are constructed by iron meshes, the top of the iron cage body is constructed by solid iron plates, baffles are arranged at the lower ends of the circumference of the iron cage body, the height H of the iron cage body is greater than the height h of each baffle, and the height h of each baffle is 10-30 cm. The method includes the steps that the iron cage body is pressed into mud on the beach, wherein the downward pressing depth of the iron cage body is greater than the height h of each baffle and smaller than the height H of the iron cage body; during a rising tide, seawater at least can submerge the beach, and then bostrichthys sinensis is put into the iron cage for starting culture. By adopting the method for culturing bostrichthys sinensis, on the one hand, the use amount of bait can be decreased, and the survival rate can be increased; on the other hand, the taste and nutritional value of obtained bostrichthys sinensis can be comparable to those of wild bostrichthys sinensis.

The invention relates to an indoor bostrichthys sinensis fry intermediate rearing method, and relates to fish fry rearing. According to the invention, pipes and air pumps are placed in a plastic tank; the plastic tank is filled with seawater; bleaching powder is added for disinfection, and the plastic tank is washed by using seawater; a sun shade net is washed by using seawater, and is exposed under sunlight; living water salinity of the fries before capturing is determined; if the living water salinity is different by no more than 10 than the seawater salinity in the plastic tank, the fries are directly placed in the plastic tank for intermediate rearing; if the salinity difference is higher than 10, the fries are subjected to 1-2 days of transition, and are transferred into the plastic tank for intermediate rearing; the fries are not fed the day the fries are placed into the plastic tank; after that, the fries are fed with bait-feed once every morning and evening; fry rearing water is replaced every two days; light intensity is kept below 6lux and seawater dissolved oxygen is maintained at (5.84+ / -0.184)mg L<-1>. When fries achieve full lengths of 8.2cm and weights of 8.0g with intermediate rearing, the fries are transferred into a culturing pond and are subjected to adult fish culturing.

The invention relates to an indoor bostrichthys sinensis fry intermediate rearing method, and relates to fish fry rearing. According to the invention, pipes and air pumps are placed in a plastic tank; the plastic tank is filled with seawater; bleaching powder is added for disinfection, and the plastic tank is washed by using seawater; a sun shade net is washed by using seawater, and is exposed under sunlight; living water salinity of the fries before capturing is determined; if the living water salinity is different by no more than 10 than the seawater salinity in the plastic tank, the fries are directly placed in the plastic tank for intermediate rearing; if the salinity difference is higher than 10, the fries are subjected to 1-2 days of transition, and are transferred into the plastic tank for intermediate rearing; the fries are not fed the day the fries are placed into the plastic tank; after that, the fries are fed with bait-feed once every morning and evening; fry rearing water is replaced every two days; light intensity is kept below 6lux and seawater dissolved oxygen is maintained at (5.84+ / -0.184)mg L<-1>. When fries achieve full lengths of 8.2cm and weights of 8.0g with intermediate rearing, the fries are transferred into a culturing pond and are subjected to adult fish culturing.

The invention discloses a method for producing functional sperm by 3D in vitro culturing of spermatogonia of C. sinensis. , and then filtered through a filter to remove undigested cell debris or cell clusters to obtain a testis single cell suspension; the testis single cell suspension was added to the percoll gradient solution for centrifugation to separate spermatogonia; the percoll gradient solution was changed from 22 to 30 % and 35~45% two concentrations of percoll solution; (2) spermatogonia are transferred to 3D culture dishes and cultured with sperm induction medium; sperm induction medium includes basal sperm medium and sex hormones, and further includes spermatogonia Melanin. The method optimizes and improves the separation, culture and induction conditions of spermatogonia, and improves the efficiency of in vitro cultured spermatogonia to produce functional sperm.

The invention discloses a marker and method for identifying single nucleotide polymorphism of a bostrichthys sinensis group and relates to a method for identifying a bostrichthys sinensis group. A single nucleotide polymorphism marker of the bostrichthys sinensis group is positioned on a 29th basic group at a 5' end of a praline tRNA gene in a bostrichthys sinensis mitochondrial genome. The method for identifying single nucleotide polymorphism of the bostrichthys sinensis group comprises the steps of: extracting a genome DNA of a bostrichthys sinensis; carrying out PCR (Polymerase Chain Reaction) amplification on the praline tRNA gene in the bostrichthys sinensis mitochondrial genome by using the DNA as a template, carrying out electrophoretic analysis on the PCR amplification products, determining that the sample is from the bostrichthys sinensis group in North of Pearl River Estuary when 330-bp PCR amplification products are tested, and determining that the sample is from the bostrichthys sinensis group in South of Pearl River Estuary when 330-bp PCR amplification products are not tested. The method for identifying single nucleotide polymorphism of the bostrichthys sinensis group is simple and easy, accurate and fast, impartial and subjective as well as economical and practical.

The invention provides a method for authenticating fresh and live bostrichthys sinensis with different sources. The method comprises the steps of shooting a photo for fresh and live bostrichthys sinensis to be measured, and obtaining traditional shape measurement data (body length, body height and the like) and frame measurement data (the spacing from the rostral side to the starting point of the first dorsal fin, the spacing from the starting point of the pelvic fin to the starting point of the first dorsal fin and the like) from the photo, and adopting the data as an original data source, conducting proportion processing on the traditional shape measurement data, conducting correction by making the frame measurement data divide the body length of the traditional shape measurement data, and substituting the result into an authentication formula so that the source of a bostrichthys sinensis sample can be effectively judged. According to the method for authenticating fresh and live bostrichthys sinensis with the different sources, through the mode of shooting the photo and obtaining the original data from the photo, the operation steps needed by authentication are effectively simplified, and the work efficiency of the authentication is improved. Meanwhile, the authentication method is high in accuracy, through the adoption of the authentication method, the authentication accuracy for fresh and live bostrichthys sinensis with a wild source, a mangrove ecological breeding source and an artificial pool breeding source can reach 93% or above.

The invention discloses a mixed bostrichthys sinensis feed which is prepared from the following raw materials in parts by weight: 70-15 parts of fish meal, 15-20 parts of puffing corn flour, 1-3 parts of soya bean lecithin, 3-5 parts of beer yeast, 3-5 parts of fish oil, 1-3 parts of rosemary powder, 0.3-0.5 part of complex vitamin, 0.3-0.5 part of complex mineral salt, 0.3-0.5 part of a growth promoting agent and 0.3-0.5 part of a complex phagostimulant. The mixed bostrichthys sinensis feed is reasonable in formula and good in water tolerance and food calling effect through mutual synergistic effect of each component. By utilizing the mixed bostrichthys sinensis feed, the growth of a fish body can be promoted, and the survival rate can be increased. The invention also provides a preparation method of the mixed bostrichthys sinensis feed, and the preparation method comprises the following steps: (1) weighing materials; (2) mixing the materials; (3) tempering and palletizing; and (4) drying and packaging. The preparation method is simple, and suitable for being used in industrial production and the nutrient loss of the mixed feed is low.

The invention discloses a method suitable for live transport of Channa sinensis, relating to a live transport method for aquatic animals. Catch S. sinensis from the breeding pond and temporarily raise it in a cement pond. During the temporary cultivation period, the pool water is oxygenated and no feeding is made to empty the food in the digestive tract of S. sinensis. When transporting in summer and autumn, gradually reduce the water temperature. During transportation in winter and spring , no need to lower the water temperature; the transport device adopts a box body, and the box body is equipped with ice cube placement holes, and ice cubes can be placed as needed; it is transported by means of transportation, and the wet wipes are covered on the surface of the snakehead snakehead during transportation to keep the fish body Keep the skin moist and maintain a suitable temperature in the box; after arriving at the destination, unload and open the box, move the Chinese snakehead into low-salinity seawater to resuscitate, and adjust the temperature of the resuscitated seawater to be similar to the temperature in the box. It has the advantages of large transport quantity per unit volume, low transport cost, simple operation and high transport efficiency. It can maintain a high survival rate during long-distance transportation.

The invention discloses soft granulated feed for bostrichthys sinensis and a production process of the soft granulated feed. According to the technical scheme, the soft granulated feed is characterized by being prepared from 120-180 parts of freshwater spotted silver carp meat, 16-24 parts of water, 16-24 parts of lactic acid bacteria, 400-600 parts of marine fish compound feed and 1-3 parts of compound vitamin. The feed has good palatability and has the function of increasing the survival rate of bostrichthys sinensis.

The invention discloses a micro-granular feed for larva fish of bostrichthys sinensis. The micro-granular feed comprises the following components in parts by weight: 50-70 parts of animal nutritional ingredients, 15-25 parts of plant nutritional ingredients, 10-25 parts of functional ingredients, 0.5-2 parts of special vitamins, 0.5-2 parts of special trace elements, 0.5-1.5 parts of casein hydrolysate and 0.5-1.5 parts of lysine. A preparation method comprises the following steps: (1) manufacturing raw materials; (2) synthesizing a biological material; (3) converting to a cooked material; (4) puffing and granulating; (5) drying; (6) spraying and coating; and (7) performing vacuum drying. The feed disclosed by the invention adopts scientific matching and an advanced processing technology is used for assistance, so that the digestion and absorption rate of the feed is effectively improved, the growth of the larva fish of bostrichthys sinensis can be effectively promoted, and the occurrence of diseases during cultivation is reduced.

The invention discloses a granular feed for juvenile fish of bostrichthys sinensis. The granular feed comprises the following components in parts by weight: 40-60 parts of animal nutritional ingredients, 20-40 parts of plant nutritional ingredients, 15-30 parts of functional ingredients, 0.3-3 parts of special vitamins, 0.3-3 parts of special trace elements, 0.2-2 parts of casein hydrolysate and 0.2-2 parts of lysine. A preparation method comprises the following steps: (1) manufacturing raw materials; (2) synthesizing a biological material; (3) converting to a cooked material; (4) puffing and granulating; (5) drying; (6) spraying and coating; and (7) performing vacuum drying. The feed disclosed by the invention adopts scientific matching and an advanced processing technology is used for assistance, so that the digestion and absorption rate of the feed is effectively improved, the growth of the juvenile fish of bostrichthys sinensis can be effectively promoted, and the occurrence of diseases during cultivation is reduced.

The invention relates to the technical field of fluorescent quantitative PCR detection methods, in particular to a primer and a real-time fluorescent quantitative PCR method for detecting the Mn-SOD gene of Chinese black snakehead (Bostrychus sinensis), which uses real-time fluorescent quantitative PCR technology to analyze Chinese black snakehead The expression of Mn‑SOD gene in different tissues of Channa sinensis, using GAPDH as an internal reference gene, designing internal reference gene primers and target gene primers; using reverse transcribed cDNA as a template, the expression of the target gene on the ABI 7500 Fast Real‑time PCR system It can quickly and accurately detect the expression and distribution of Mn-SOD gene in different tissues of Channa sinensis, and provide a scientific basis for the utilization and research of this gene.