A method for identifying live and fresh Channa sinensis from different sources
A technology of Chinese black pond snakehead and source, applied in the application, fish farming, animal husbandry and other directions, can solve the problems of ecological breeding of Chinese black pond snakehead nutritional value and economic value difference, difficult to realize artificial breeding, low identification accuracy and other problems , to achieve the effect of no professional requirements, high accuracy, simple and convenient operation
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Embodiment 1
[0072] Samples and sources: In September 2016, a total of 84 live Snakena chinensis were collected, 28 from wild sources, 28 from mangrove ecological culture sources and artificial pond culture, of which:
[0073] Wild populations were purchased from Shanxin Village, Jiangping Town, and fishermen caught them with centipede cages.
[0074] The mangrove ecological breeding population was provided by the Guangxi Mangrove Research Center Pearl Harbor Coastal Ecological Experiment Station located in Shijiaobu, Jiangshan Township. The seedlings were released in April 2016. The size of the seedlings was 27 / 500g, and the source of the seedlings was wild. The breeding method is as follows: a pipeline is buried in the soil under the mangrove forest, and there is long flowing water in the pipeline. Part of the bait for cultured Chinese snakehead comes from artificial feeding, and the rest comes from natural small fish, shrimp and crab shells in the natural water flowing through the pipeli...
Embodiment 2
[0099] Samples and sources: In April 2017, 51 living bodies of Channa sinensis were collected, including 11 from wild sources, 20 from mangrove land pipeline ecological breeding, and 20 from artificial pond breeding. The specific breeding methods of each sample are the same as in Example 1 .
[0100] 1. Anesthesia:
[0101] The fish used in the experiment were transported back to the laboratory in a foam box to moisturize and dry, and they were raised temporarily in semi-seawater with a salinity of 15‰, and were processed and measured within 24 hours. Anesthetize the fish to be tested with semi-seawater containing 60-100 mg / L ethyl m-aminobenzoate methanesulfonate (MS-222) before measurement for 8-10 minutes, and measure after the water on the surface of the fish is blotted with absorbent paper. The salinity of semi-sea water is 12-20‰.
[0102] 2. Sample shooting:
[0103] Fix the digital camera (Canon G12) on the top of the table with a tripod, make the lens vertical to t...
Embodiment 3
[0123] Samples and sources: In May 2017, 87 live Snakena chinensis were collected, including 27 from wild sources, 30 from mangrove ecological culture, and 30 from artificial pond culture. The specific breeding methods of each sample are the same as in Example 1.
[0124] 1. Sample shooting:
[0125] Use a selfie stick to fix the mobile phone (HUAWEI MATE8) with camera function above the table, make the lens perpendicular to the table, place the fresh and alive Chinese snakehead to be tested side up on the table, and align the middle of the side of the fish with the center of the lens , adjust the distance between the lens and the table so that the complete fish body in the photo occupies about three-quarters of the entire picture, take a photo, and place a label marked with the sample number next to each fish.
[0126] 2. Morphometric measurements
[0127] Input the photo of the sample into the computer, and use Image-Pro Plus6.0 software to perform morphological measurement...
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