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Gene, protein, polyclonal antibody and application of vitellogenin

A thorny sea car and polyclonal antibody technology, which is applied in the fields of application, genetic engineering, immunoglobulin, etc., can solve the problems of marine environmental pollution indication, and achieve the effects of slow movement, strong sensitivity and specificity

Active Publication Date: 2022-05-20
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, most of the researches on thorny sea pens have focused on prevention, hunting, medicinal value, and analysis of nutritional components, but little research has been done on the indication of marine environmental pollution.

Method used

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  • Gene, protein, polyclonal antibody and application of vitellogenin
  • Gene, protein, polyclonal antibody and application of vitellogenin
  • Gene, protein, polyclonal antibody and application of vitellogenin

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Embodiment 1

[0025] 1. Acquisition of Vg full-length cDNA

[0026] The full-length cDNA sequence of the target gene Vg was cloned by RACE technology, with a total of 6074bp, encoding a protein of 220KDa, and the length of the open reading frame (Open Reading Frame, ORF) was 5805bp. DNAMAN, Phylogenetic, InterViewer and other bioinformatics software were used to identify the sequence in terms of homology comparison, cluster analysis, and amino acid primary structure alignment, etc., and it was proved that the sequence was the target gene, the Vg gene sequence See SEQ IP NO.1.

[0027] 2. Preparation of polyclonal antibody against P. spinosa Vg

[0028] Since the Vg gene sequence is as long as 6074bp, and its protein molecular weight exceeds 220KDa, it is difficult to construct a prokaryotic expression vector for recombinant expression. Therefore, we synthesized Vg polypeptide as an antigen, and then prepared polyclonal antibodies.

[0029] 2.1 Preparation of polypeptides:

[0030] Analy...

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Abstract

The present invention relates to the gene, protein, polyclonal antibody and application of the vitellogenin of the spiny sea plate, and belongs to the field of molecular biology. The gene sequence of the spinous sea plate vitellogenin is SEQ ID NO: 1, and the protein is as follows: Described in SEQ ID NO:2. Utilize the ELISA method of the Vg antibody of the spiny carp Vg, by identifying the Vg content in the organism, and then warn the pollution status of EEs in the marine ecosystem, the detection kit prepared by using the spiny spiny Vg antibody can detect a certain The degree of estrogen pollution in the sea area is accurate and reliable.

Description

technical field [0001] The invention belongs to the detection field of marine pollutants, and in particular relates to a gene, protein, polyclonal antibody and application of encoding vitellogenin of the spiny sea disc. Background technique [0002] Vitellogenin (Vg) is a high-molecular-weight protein containing sugar, phosphorus and lipid that is specifically present in oviparous non-mammalian sexually mature animals. Its main function is to serve as the precursor of vitellin, providing nutrients and functional substances such as amino acids, fats, carbohydrates, vitamins, phosphorus and sulfur for embryonic development. In addition, it also has the functions of immune defense, promoting the growth and differentiation of oocytes, carrier transport and enzyme activity. There is an estrogen response element (Estrogen Receptor Element, ERE) in the regulatory region of the Vg gene. After estrogen binds to the receptor, it forms a dimer, recognizes and binds this sequence, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/12C07K14/435C07K16/18C07K16/06G01N33/68
CPCC07K14/43504C07K16/18C07K16/065G01N33/68G01N2333/43504Y02A50/30
Inventor 李风铃于永兴姚琳江艳华杜美荣李兆杰王联珠曲梦
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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