98 results about "NUCLEIC ACID SUBSTANCE" patented technology

The invention provides a method and a system for digital quantitative analysis of nucleic acid amplification based on micro-droplet. The method comprises the following steps: preparing a to-be-detected nucleic acid amplification reaction liquid which includes a to-be-detected nucleic acid template, a reaction buffer water solution, deoxyribonucleoside triphosphate, a primer, polymerase and a product marking substance; loading the prepared to-be-detected nucleic acid amplification reaction liquid in a micro-pipeline of which the two ends are both provided with an opening, wherein the micro-pipeline is arranged above an open container, and the open container contains an oily liquid containing a surfactant; enabling the opening of one end of the micro-pipeline to do up-and-down reciprocating vibration on the surface of a liquid in the open container or do leftward-and-rightward reciprocating vibration below the liquid surface so as to generate a plurality of droplets which are flatly laid at the bottom of the open container; performing nucleic acid amplification reaction on the plurality of droplets in the open container; acquiring product signals generated after the nucleic acid amplification reaction is ended, and performing quantitative analysis on the nucleic acid template. The digital nucleic acid amplification analysis method can be used for implementing quantitative detection on low-concentration nucleic acid substances in a micro-system and is simple and convenient to operate, high in efficiency and low in cost.

The invention discloses a method and application for preparing a tissue engineering corneal carrier stent by utilizing fresh porcine cornea. The method comprises the following steps: cutting a fresh porcine cornea for swelling, repeatedly freeze thawing and breaking cells, removing residual nucleic acid substances through DNA-RNA enzyme treatment, cross-linking, airing, and finally performing irradiation sterilization through ionization rays, thereby obtaining the product. The method is applied to carrier stents of tissue engineering corneal epithelium, stroma, endothelium, front board layer half cornea, rear board layer half cornea and full-layer board layer half cornea, so that the requirement on batch production of tissue engineering corneas is met. The carrier stent serves as a substitute of human corneal stroma to be used for clinical transplant and treatment of un-accumulated whole layer keratohelcosis. The method is applied to large-scale production and clinical popularization and application. The prepared carrier stent has the characteristics of high transparency, dense structure, high mechanical property, high biocompatibility with human corneal seed cells and the like. Therefore, the method is suitable for batch production, so that the requirements on lots of carrier stents for tissue engineering corneas are met.

The invention relates to the blood cell detection field and discloses a blood cell detection reagent, a blood cell processing method and a blood cell identification method. The blood cell detection reagent comprises fluorescent dye, a sphericized component and organic alcohol, wherein the fluorescent dye has cell permeability and can specially stain the nucleic acid substance in the cell; the sphericized component can sphericize red blood cells, keep the red cell membrane to be intact and cannot damage the internal structure of the white blood cell; the organic alcohol can enhance the cell permeability and aids the fluorescent dye to enter the cells. The invention also discloses a blood cell processing method and a blood cell identification method. According to the blood cell detection reagent and the method, one kind of reagent can detect different blood cells at one time, and can particularly detect juvenile white blood cells and reticulated red blood cells simultaneously, therefore, the detection cost is greatly saved, the detection speed is increased and the instrument complexity is reduced.

The present invention relates to a process for the concentration and / or isolation of nucleic acids or nucleic acid-containing species from a nucleic acid-containing solution, and a kit therefor. In one embodiment, the invention relates to the concentration and / or isolation of DNA and RNA from nucleic acid-containing solutions.

The invention discloses a double labelling Nano-Au probe and a preparation method and application thereof. The probe comprises a Nano-Au particle as a core, the surface of which is connected with antibody and oligonucleotide simultaneously. The preparation method of the double labelling Nano-Au probe includes the following steps: (1) coupled reaction: adding oligonucleotide solution and antibody solution in Nano-Au sol with certain concentration and uniformly mixing the solutions to lead the Nano-Au particle, the antibody and nucleic acid to fully act in the solution; and (2) stabilizing and blocking reaction: preparing PBS buffer solution with the content of SDS being 5-10 percent and then adding the PBS buffer solution in the mixed solution prepared in step (1) and adding sealant to form nano probe sol. The double labelling Nano-Au probe can convert the antigenic detection into the detection of nucleic acid matter and is applied to the detection of tumor marker, hormone and pathogene micro-protein.

The invention relates to a lumbricus extract possessing antithrombotic effect, which takes lumbricus decoction pieces as a raw material. The preparation method comprises the following steps: coarse cracking the raw material of the lumbricus decoction pieces, immersing for 4-5 hours by adding 5-15 times water by weight, adding immersion liquid together in a pulverizer to crush by a wet method so as to obtain slurry with average particle fineness of D50<=100 mum, centrifuging and taking a supernatant, passing through a ceramic membrane with average aperture of 0.45 mum, refrigerating a filtrateat the temperature less than or equal to minus 20 DEG C for 2 hours, and then refrigerating and drying the filtrate under the condition of minus 4 DEG C-minus 6 DEG C of temperature and 0.1mbr-0.05mbr of absolute pressure. The lumbricus extract is capable of extracting amino acid and nucleic acid substances, and keeping the activity of effective chemical ingredients of protein and polypeptide with maximum limit. The lumbricus extract has dual effects of anticoagulation and thromboclasis, animal experiments show that the lumbricus extract has the advantages of good antithrombotic effect and simple extraction method with high efficiency.

The invention discloses a virus detection system and a micro-fluidic chip thereof. The micro-fluidic chip comprises a liquid storage unit used for storing a liquid used or generated in the detection process, a nucleic acid purification unit communicated with the liquid storage unit and used for purifying a sample liquid to obtain a nucleic acid substance, and an amplification detection unit comprising a main pipeline, a plurality of reaction tanks and a plurality of branch pipelines, wherein the amplification detection unit is communicated with the nucleic acid purification unit through the main pipeline, the reaction tanks are communicated with the main pipeline through the branch pipelines, and the reaction tanks are used for amplifying and detecting nucleic acid substances of differentsample solutions. The multifunctional micro-fluidic chip integrates nucleic acid extraction, nucleic acid purification and nucleic acid amplification and detection. The requirements of all steps of nucleic acid amplification and detection can be automatically met on the premise of one-time sample introduction, and a plurality of reaction tanks are arranged so that amplification and detection of aplurality of sample solutions can be achieved at the same time and the detection efficiency is high.

The invention discloses a nucleic acid substance carrier containing a degradable imine linkage. The structural formula of the nucleic acid substance carrier containing the degradable imine linkage is described in the specification, wherein x is 1-20, y is 1-20, and n is 1-20. The invention also discloses a preparation method of the nucleic acid substance carrier containing the degradable imine linkage, an application in nucleic acid medicine delivery and a complex. Compared with the prior art, the nucleic acid substance carrier which contains an imine linkage structure and can degrade low-molecular-weight PEI (polyether imide) derivatives is simple in structure and easy to synthesize, shows higher transfection activity in different cell strains and has lower cell toxicity, so that the nucleic acid substance carrier is a high-efficiency and low-toxicity gene delivery carrier.

The invention provides a micro-volume cell nucleic acid amplification method which comprises the following steps: a) putting micro liquid droplets with a small amount of cells inside into a small-sizecontainer with an oil phase supplied in advance, and performing centrifugation to settle down the micro liquid droplets; b) putting cell lysis buffer droplets into the small-size container through micro-volume injection below the liquid level of the oil phase, performing centrifugation to settle down the cell lysis buffer droplets, and fusing the cell lysis buffer droplets with cell droplets so as to achieve splitting of cells and release of nucleic acid substances; c) adding splitting termination droplets through micro-volume injection, performing centrifugation, fusing the splitting termination droplets with the split cell droplets, and neutralizing or terminating the splitting reaction; d) adding one or more amplification reaction liquid through micro-volume injection, performing centrifugation fusion, and performing amplification on genomes, transcriptome or specific nucleotide sequences at an appropriate temperature. The micro-volume cell nucleic acid amplification method provided by the invention is simple in operation, low in cost and high in flux, and the reaction volume can be reduced to a nano liter grade.

The invention relates to a circulation free DNA and blood cell preservation medium which comprises the following components: 1) one or more protection agents; 2) one or more chelation stabilizers; 3)one or more metabolic inhibitors; 4) one or more enzyme inhibitors; 5) one or more anti-adsorption agents; 6) a buffer solution. The preservation medium has a remarkable circulation free DNA and cellprotection effect, is capable of preserving a blood preparation or a biological sample for a long time at a normal temperature and is free of free aldehyde substance, anaphylactic reactions and toxicreactions of human bodies in contact with liquid samples and biological samples with the preservation medium are reduced, potential carcinogenic risks of the preservation medium are reduced, the completeness of nucleic acid in a product preparation and a biological sample can be maintained, accuracy and scientificity of nucleic acid substance detection can be ensured, and detection results approximate to reality can be provided.

The invention provides a micro-fluidic chip and a nucleic acid extraction and purification method therewith. The micro-fluidic chip has a four-layer membrane structure including: a liquid path layer, a gas path control layer, a magnet control layer and a glass substrate layer. The gas path control layer is arranged below the liquid path layer. The substrate layer is arranged below the gas path control layer. The magnet control layer is arranged below the glass substrate layer. The method, with the micro-fluidic chip, includes the steps of: a) introducing a target sample; b) introducing superpara-magnetic silicon beads for nucleic acid purification; and c) extracting and purifying nucleic acid from the mixed sample. In the invention, introduction of the sample, cleaning of non-nucleic acid substances and collection and purification of the nucleic acid are integrated on one micro-fluidic chip, so that the nucleic acid extraction and purification process is optimized and extraction and purification efficiency is greatly improved. The method simplifies manual operations and achieves temporal-spatial resolution information which is difficult in conventional methods, and has excellent nucleic acid extraction and purification effects.

The invention relates to the technical field of nucleic acid extraction, in particular to a rapid nucleic acid extraction device and a method for extracting nucleic acid. The rapid nucleic acid extraction device comprises a rapid nucleic acid extractor, a lysis solution, a washing solution and an eluent, the eluent is used for eluting and collecting nucleic acid substances adsorbed on the rapid nucleic acid extractor, and the washing solution is one or a mixture of more of silicone oil, paraffin oil, C5-C15 alkane oil and halogenated C1-C15 alkane oil. The washing solution does not contain alcohol, does not need to be aired, does not influence nucleic acid, and ensures quick and effective detection.

The invention provides a method and a system for digital quantitative analysis of nucleic acid amplification based on micro-droplet. The method comprises the following steps: preparing a to-be-detected nucleic acid amplification reaction liquid which includes a to-be-detected nucleic acid template, a reaction buffer water solution, deoxyribonucleoside triphosphate, a primer, polymerase and a product marking substance; loading the prepared to-be-detected nucleic acid amplification reaction liquid in a micro-pipeline of which the two ends are both provided with an opening, wherein the micro-pipeline is arranged above an open container, and the open container contains an oily liquid containing a surfactant; enabling the opening of one end of the micro-pipeline to do up-and-down reciprocating vibration on the surface of a liquid in the open container or do leftward-and-rightward reciprocating vibration below the liquid surface so as to generate a plurality of droplets which are flatly laid at the bottom of the open container; performing nucleic acid amplification reaction on the plurality of droplets in the open container; acquiring product signals generated after the nucleic acid amplification reaction is ended, and performing quantitative analysis on the nucleic acid template. The digital nucleic acid amplification analysis method can be used for implementing quantitative detection on low-concentration nucleic acid substances in a micro-system and is simple and convenient to operate, high in efficiency and low in cost.

To provide a new technique by which efficient transfection is ensured in delivering a target gene into a cell, disclosed is a nucleic acid construct for nuclear import, which comprises a ternary complex consisting of a nucleic acid substance containing a gene to be delivered into the nucleus of a cell, an importin protein (for example, importin-β) capable of passing through the nuclear pore and involved in the nuclear transport, and a binding substance (for example, polyethyleneimine) bound to both of the nucleic acid substance and the importin protein. Nucleic acid transport from outside of a cell into the cell nucleus can be particularly promoted by administering the nucleic acid construct bound to a cell membrane receptor binding factor and / or a membrane fusing substance, or administering the nucleic acid construct encapsulated in a non-viral vector (for example, Sendai virus envelope) having cell membrane permeability and membrane fusing properties.

The invention discloses a method for efficiently extracting high-quality nucleic acid substances from olive leaves. The operation steps mainly include sampling, grinding, pyrolysis, impurity removal,precipitation, purification, dissolution and the like. Aiming at the characteristics that the olive is rich in impurities such as polyphenols, polysaccharides and proteins, on the basis of a traditional CTAB method, deep optimization is carried out on the characteristics of olive leaves, systematic improvement is carried out on sample dosage, reagent types, reagent concentration, reaction time, reaction volume, operation steps and the like, and a method suitable for high-efficiency and high-quality extraction of nucleic acid substances of olive tender leaves, mature leaves or old leaves is developed. The method can select and extract olive leaf genome DNA or total RNA according to actual requirements, has wide applicability and strong practicability, and has good application value.

The present teachings are generally directed to methods for normalizing at least one species of small nucleic acid that is present in a population of small nucleic acid species, wherein the relative concentration of at least one small nucleic acid species is substantially greater than the relative concentration of at least one other small nucleic acid species in the population. At least one small nucleic acid species is normalized using a multiplicity of primers comprising degenerate sequences. In some embodiments, a small nucleic acid species is identified by inserting at least part of an extension product from a normalized population into a vector and subsequently sequencing the insert. In some embodiments, a small nucleic acid species is identified by determining the sequence of at least part of an extension product.

The invention discloses an aeromonas strain R1, a preparation method of the aeromonas strain and the application of the aeromonas strain in algae lysing and microcystin degradation. The strain R1 is obtained by being screened from rotten algal bloom in the mirror lake, the strain is high in cadmium resistance, can be applied to algae lysing and can degrade microcystins, and through 16SrDNA sequence comparison, analysis and identification, the R1 belongs to aeromonas. The strain has good tolerance to Cd2+, and can still grow normally in a medium containing 50 mg / L Cd2+; the strain lyses microcystis aeruginosa by secreting extracellular substances, and the substances are resistant to high temperature and refer to non-nucleic-acid substances, polysaccharides and other substances. In the presence of 0.1 mg / L Cd2+, the algae lysing capacity of the strain is enhanced; when the initial concentration of microcystins is 1.84 mg / L, the degradation rate is 43.5% within seven days.

The invention provides a novel method for extracting phage genome DNA. The novel method comprises the following steps: after amplifying phage lysate, removing cell debris and nucleic acid substances of host bacteria, adding PEG6000 to settle phage, after resuspending by TM buffer liquid, avoiding chloroform extraction, directly adding nuclease, removing the genome of the remaining host bacteria, then replacing protease K by using urea, after degeneration of coat protein, separating the protein from the genome DNA by agarose gel electrophoresis, and then recycling the genome DNA of the phage byusing a freeze thawing and recycling method. The method is simple and convenient to operate, is particularly suitable for phage sensitive to chloroform, and is wide in application range, experiment costs are greatly reduced, and extracting time is shortened.

The invention provides application of Wnt2 to preparation of a medicament for inhibiting the esophageal cancer, and provides a medicament for inhibiting the development of the esophageal cancer. The medicament comprises a component for inhibiting the action of a Wnt2 gene or / and a Wnt2 protein. The component comprises one or more a polypeptide substance capable of enclosing the Wnt2 gene, an antibody / ligand capable of enclosing the Wnt2, a lethal medicament or a nucleic acid substance carried by an antibody / ligand capable of inhibiting or killing the Wnt2 and a small interfering ribonucleic acid (siRNA) or a miRNA (micro Ribonucleic Acid) or a shRNA (short hairpin Ribonucleic Acid) capable of interfering / blocking the Wnt2 gene. The invention has the advantage: the application of the Wnt2 gene to the preparation of a medicament for inhibiting the esophageal cancer is provided, a WNT signal conduction path in a WNT2 activated tumor cell is blocked under the silencing action or the neutralizing action of an antibody, so that the effect of inhibiting the growth of tumors is achieved.

The invention belongs to the technical field of biochemistry, and provides a method for extracting nucleic acid from bee pollen and purifying. The specific method is as follows: freezing screened, purified and dried bee pollen, and ultrafine grinding until the particle size achieves 20-60 meshes, adding a 0.14mol / L sodium chloride solution with weight ratio of 1: (8-10), homogenating, separating organelle through differential centrifugation, and ultrasonically treating for 30-40 minutes at 20-30 KHz, cracking to obtain DNA-proteins, then adding a 1mol / L sodium chloride solution with weight ratio of 1: (6-8), homogenating, ultrasonically treating for 30-40 minutes at 20-30 KHz, cracking to obtain RNA-nucleoproteins, adding a proper amount of sodium dodecyl sulfate to separate the proteins from nucleoproteins, adding concentrated potassium acetate, precipitating a sodium dodecyl sulfate-protein complex, transforming spare sodium dodecyl sulfate as sylvite with small solubility and simultaneously precipitating, repeatedly extracting, obtaining a nucleic acid pure solution through ultracentrifugation or ion-exchange column chromatography, and performing vacuum freezing and drying on the nucleic acid pure solution to obtain the pure nucleic acid substance.

The invention discloses a method for comprehensively judging an autolysis condition of beer yeast and belongs to the field of evaluation of yeast quality. According to the method, the (A260 / A280) / death rate is used as an index for judging the autolysis condition of the yeast; based on a basic ultraviolet spectrophotometry and a cytometry, the method is simple, accurate and high in repetitiveness. Compared with the conventional reported yeast autolysis judgment method, the method disclosed by the invention has the advantages that an experiment result can be obtained within extremely short time, so that the workload and the working time are greatly reduced, and the experiment result is comprehensive and real. The A260 / A280 reflects the proportion of protein substances to nucleic acid substances in an autolysis solution and relevant content of the protein substances and the nucleic acid substances and is an extremely comprehensive index, the conventional single-index boundedness is overcome, and the manmade operation error is small. Meanwhile, due to the final judgment index (A260 / A280) / death rate, the influence, caused by different death rates, on the result is avoided; the experiment result is objective and real. The method disclosed by the invention can be used for evaluating the autolysis degree of the same type of yeast and comparing the autolysis performance of different types of yeasts.

The invention relates to dsRNA in an organism or tissue and a method for extracting the dsRNA. The method comprises the following steps: pre-treating, centrifuging to obtain supernate, carrying out chromatography on the supernate, carrying out segmented elution, and collecting eluent for post-treatment. According to the method, organic reagent treatment and cellulose column chromatography are combined, firstly, an organism or tissue is treated to be broken, and nucleic acid substances are released into a liquid phase; and by means of reversible adsorption of filler in cellulose column chromatography on liquid-phase medium-concentration nucleic acid substances, dsRNA and other nucleic acid substances can be effectively separated through segmented elution, especially interference of DNA degradation substances can be remarkably reduced, and the purity of the final product dsRNA can be improved.

The invention relates to the technical field of biological materials, in particular to a preparation method of decellularized heterogeneous small blood vessels. By adopting a method for combining ultrasound, perfusion, detergent and enzyme, cells in a biological material matrix are broken through a cavitation effect of high-frequency ultrasonic waves, then the detergent is pressed into a materialin a perfusion mode to further wash cell components and part of immunogenic substances; after the primary action of ultrasound and the detergent, the cells are basically broken, most of cell components are washed away, most of nucleic acid substances are further removed under the action of DNase-I and RNase-A, and the residual quantity is less than 100 ng / mg; and finally, a good decellularizationeffect is achieved through repeated sterile PBS solution lavage.

The invention provides a biological detection method based on an excitation light source. The biological detection method comprises the following steps of: injecting a sample into a sample adding holeformed in a sample adding layer; connecting a gasket arranged below the sample adding layer with a pipeline layer at the lower end through a buckle structure, during use, pulling out the gasket alonga rail, pressing the sample adding layer downwards to connect the pipeline layer with the sample adding layer in a clamped mode, sequentially mixing a row of pricking needles arranged on the pipelinelayer with a reagent of the sample adding layer, and carrying out extraction and purification reaction in the pipeline layer, then introducing nucleic acid substances in the sample into an amplification bin arranged on the pipeline layer; enabling an excitation light module to emit exciting light with a preset wavelength to an amplification bin, carrying out reaction, then acquiring fluorescenceinformation through a fluorescence sensor to complete detection, and controlling the temperature in the amplification bin to be within a preset range by the temperature control module, thus enabling areagent of a chip device to reach an optimal reaction state.

The invention provides a eugenol combination liquid as well as a preparation method and an application of the eugenol combination liquid. The combination liquid comprises the following raw materials by volume: 99.8555%-99.9711% of water, 0.01%-0.05% of Tween 80 solution, and 0.0189%-0.0945% of eugenol; and the invention provides the method for preparing the eugenol combination liquid. The eugenol combination liquid inhibits the growth of staphylococcus aureus, and can even be used as a bactericide when the concentration of the eugenol is higher. The eugenol combination liquid affects the molecular structures of an SDH enzyme and an MDH enzyme of staphylococcus aureus, and the staphylococcus aureus is changed, so that the conformation is changed, and the activity of the enzyme is reduced. After the eugenol combination liquid acts on the staphylococcus aureus, the nucleic acid substances related to the synthesis of protein are leaked because of the change of cell permeability, the synthesis of the protein is further inhibited, and finally the cell death is caused.

The invention provides a method of enhancing the action of a pharmaceutical agent selected from the group consisting of the anti-infective agents, the group comprising of the antimicrobial agents, the anthelmintic agents and the anti-ectoparasitic agents, but excluding coal tar solution and H1-antagonist antihistamines, and from the group consisting of the CPNS agents selected from the group of compounds acting on the central or peripheral nervous system, but excluding coal tar solution and H1-antagonist antihistamines and also excluding anti-inflammatory, analgesic and antipyretic agents and also provides an enhanced method for the administration of a nucleic acid substance to the cells of an animal, a plant or a micro-organism. The method is characterized in that the agent or nucleic acid substance is formulated with an administration medium which comprises a solution of nitrous oxide gas in a pharmaceutically acceptable carrier solvent for the gas and which administration medium includes at least one fatty acid or ester or other suitable derivative thereof selected from the group consisting of oleic acid, linoleic acid, alpha-linolenic acid, gamma-linolenic acid, arachidonic acid, eicosapentaenoic acid [C20:5ω3], decosahexaenoic acid [C22:6ω3], ricinoleic acid and derivatives thereof selected from the group consisting of the C1 to C6 alkyl esters thereof, the glycerol-polyethylene glycol esters thereof and the reaction product of hydrogenated natural oils composed largely of ricinoleic acid based oils, such as castor oil with ethylene oxide. The formulations of such agents or substances form part of the invention.

The invention discloses a magnetic bead mixed liquid split charging device and method for nucleic acid substance extraction. The device comprises a shell, a stirring device is arranged in the shell, the stirring device comprises a stirring part for stirring, the stirring part is arranged at the middle lower part of an inner cavity of the shell, the stirring part is arranged in the shell, the upper end of the stirring part is fixedly connected with the lower end of a suction pipe of a tubular structure, the upper end of the suction pipe is communicated with a negative pressure device through a conveying pipe, a rotating device used for providing rotating force power is arranged on the suction pipe, the negative pressure device and the rotating device are arranged outside the shell, the rotating device drives the stirring part to rotate through the suction pipe, and the suction pipe is used for discharging the magnetic bead mixed liquid in the shell. By means of the split charging device and the split charging method, automatic even mixing and automatic split charging of the magnetic bead mixed liquid can be achieved, the adsorption performance of the magnetic beads can be guaranteed under the low-temperature condition, time and labor are saved, operation is standard, cost is low, and the device and the method are particularly suitable for industrial production.

The invention discloses a double-labeled nano-gold probe, its preparation method and application. The probe includes: the gold nanoparticle is the core, and the surface is simultaneously connected with antibodies and oligonucleotides. The preparation method of the double-labeled nano-gold probe includes the following steps: 1. Ligation reaction: add a concentration of oligonucleotide solution and antibody solution to the nano-gold sol, mix evenly, and make the nano-gold particles and antibody in the solution 2. Nucleic acid can fully function to make a mixed solution; ② Stabilization and blocking reaction: prepare a PBS buffer solution containing 5-10% of SDS, then add it to the mixed solution made in step ①, add a blocking agent, and form a nano Sol of the probe. The double-labeled nano-gold probe can transform the detection of antigens into the detection of nucleic acid substances, and is applied to the detection of tumor markers, hormones, and trace proteins of pathogens.

The invention relates to the field of biological equipment manufacturing engineering and discloses a biological sample extraction device and method. The device comprises a nucleic acid storage area, a mobile phase storage area, a chromatographic column, a temperature control component, a DNA collecting area and an RNA collecting area, wherein the nucleic acid storage area and the mobile phase storage area are connected with an inlet of the chromatographic column, an outlet of the chromatographic column is connected with the DNA collecting area through a switch I and connected with the RNA collecting area through a switch II, and the temperature control component wraps the chromatographic column. With the adoption of the device and the method, the manual operation involvement degree is decreased, the automatic operation degree is high, and large-scale nucleic acid substance separation is facilitated.