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Method for extracting nucleic acid from bee pollen and purifying

A bee pollen and nucleic acid technology, applied in the field of biochemistry, can solve the problems of large residual amount of harmful substances, unable to meet the needs of the human body, and low nucleic acid extraction rate, and achieve the effects of low cost, short production cycle and high extraction rate

Inactive Publication Date: 2013-05-15
大兴安岭绿源蜂业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The purpose of the present invention is to provide a method to make the human body absorb more nutritional components of bee pollen, solve the problem of low nucleic acid extraction rate, large residual amount of harmful substances, limited application range, and daily diet that cannot meet the needs of the human body

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0020] The extraction method of the present invention is as follows: freezing the bee pollen after screening, removing impurities and drying for ultrafine pulverization, the particle size reaches 40-60 mesh, adding 0.14mol / L sodium chloride solution with a weight ratio of 1:8-10, Homogenize, separate the organelles by differential centrifugation, and ultrasonicate at 20-30 kHz for 30-40 minutes to cleavage it to obtain DNA-protein, then add 1mol / L sodium chloride solution with a weight ratio of 1:6-8 , homogenate, and ultrasonic at 20-30 kHz for 30-40 minutes to crack it to obtain RNA-nucleoprotein, add an appropriate amount of sodium dodecylsulfonate to separate protein and nucleic acid, add concentrated potassium acetate to make dodecyl Sodium sulfonate-protein complex is precipitated, and the excess sodium dodecyl sulfonate is converted into a potassium salt with low solubility and precipitated at the same time, repeated extraction, and a pure nucleic acid solution is obtain...

Embodiment 2

[0022] The extraction method of the present invention is as follows: freezing the bee pollen after screening, removing impurities and drying for ultrafine pulverization, the particle size reaches 20-40 mesh, adding 0.14mol / L sodium chloride solution with a weight ratio of 1:8-10, Homogenize, separate the organelles by differential centrifugation, and ultrasonicate at 20-30 kHz for 30-40 minutes to cleavage it to obtain DNA-protein, then add 1mol / L sodium chloride solution with a weight ratio of 1:6-8 , homogenate, and ultrasonic at 20-30 kHz for 30-40 minutes to crack it to obtain RNA-nucleoprotein, add an appropriate amount of sodium dodecylsulfonate to separate protein and nucleic acid, add concentrated potassium acetate to make dodecyl Sodium sulfonate-protein complex is precipitated, and the excess sodium dodecyl sulfonate is converted into a potassium salt with low solubility and precipitated at the same time, repeated extraction, and a pure nucleic acid solution is obtain...

Embodiment 3

[0024] The extraction method of the present invention is: freezing the bee pollen after screening, removing impurities and drying for ultrafine pulverization, the particle size reaches 20-60 mesh, adding 0.14mol / L sodium chloride solution with a weight ratio of 1:10-12, Homogenize, separate the organelles by differential centrifugation, and ultrasonicate at 20-30 kHz for 30-40 minutes to cleavage it to obtain DNA-protein, then add 1mol / L sodium chloride solution with a weight ratio of 1:6-8 , homogenate, and ultrasonic at 20-30 kHz for 30-40 minutes to crack it to obtain RNA-nucleoprotein, add an appropriate amount of sodium dodecylsulfonate to separate protein and nucleic acid, add concentrated potassium acetate to make dodecyl Sodium sulfonate-protein complex is precipitated, and the excess sodium dodecyl sulfonate is converted into a potassium salt with low solubility and precipitated at the same time, repeated extraction, and a pure nucleic acid solution is obtained by ultr...

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Abstract

The invention belongs to the technical field of biochemistry, and provides a method for extracting nucleic acid from bee pollen and purifying. The specific method is as follows: freezing screened, purified and dried bee pollen, and ultrafine grinding until the particle size achieves 20-60 meshes, adding a 0.14mol / L sodium chloride solution with weight ratio of 1: (8-10), homogenating, separating organelle through differential centrifugation, and ultrasonically treating for 30-40 minutes at 20-30 KHz, cracking to obtain DNA-proteins, then adding a 1mol / L sodium chloride solution with weight ratio of 1: (6-8), homogenating, ultrasonically treating for 30-40 minutes at 20-30 KHz, cracking to obtain RNA-nucleoproteins, adding a proper amount of sodium dodecyl sulfate to separate the proteins from nucleoproteins, adding concentrated potassium acetate, precipitating a sodium dodecyl sulfate-protein complex, transforming spare sodium dodecyl sulfate as sylvite with small solubility and simultaneously precipitating, repeatedly extracting, obtaining a nucleic acid pure solution through ultracentrifugation or ion-exchange column chromatography, and performing vacuum freezing and drying on the nucleic acid pure solution to obtain the pure nucleic acid substance.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, in particular to a method for extracting and purifying nucleic acid from bee pollen. [0002] The raw material bee pollen used in this product comes from the primeval forest of Daxing'an Mountains. It is located between 50 degrees and 53 degrees north latitude. It is a natural pure land with a large temperature difference between day and night, which is suitable for the growth of wild flowers. The vegetation here is lush and rich in species. On the 84,000 square kilometers of land, there are thousands of wild plants, including 59,850 mu of honey, powder, and glue source plants, and the annual reserves of bee products can reach 840,000 tons. The raw materials of this product are all in line with the organic food production and processing standards of the EU organic food certification organization, and it is a safe functional nutritional food. Background technique [0003] Bee pollen is com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H21/00C07H1/08
Inventor 徐春华孟庆生
Owner 大兴安岭绿源蜂业有限公司
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