Method and application for preparing tissue engineering corneal carrier stent by utilizing fresh porcine cornea

Abstract

The invention discloses a method and application for preparing a tissue engineering corneal carrier stent by utilizing fresh porcine cornea. The method comprises the following steps: cutting a fresh porcine cornea for swelling, repeatedly freeze thawing and breaking cells, removing residual nucleic acid substances through DNA-RNA enzyme treatment, cross-linking, airing, and finally performing irradiation sterilization through ionization rays, thereby obtaining the product. The method is applied to carrier stents of tissue engineering corneal epithelium, stroma, endothelium, front board layer half cornea, rear board layer half cornea and full-layer board layer half cornea, so that the requirement on batch production of tissue engineering corneas is met. The carrier stent serves as a substitute of human corneal stroma to be used for clinical transplant and treatment of un-accumulated whole layer keratohelcosis. The method is applied to large-scale production and clinical popularization and application. The prepared carrier stent has the characteristics of high transparency, dense structure, high mechanical property, high biocompatibility with human corneal seed cells and the like. Therefore, the method is suitable for batch production, so that the requirements on lots of carrier stents for tissue engineering corneas are met.

Description

technical field [0001] The invention relates to a method and application for preparing a tissue engineering cornea carrier bracket by using fresh pig cornea, and belongs to the preparation technology of the cornea carrier bracket in the field of biological tissue engineering. Background technique [0002] The cornea is a transparent membrane located at the very front of the eyeball, covering the iris, pupil, and anterior chamber, and provides most of the refractive power for the eye. Because the cornea is in direct contact with the external environment, it is extremely vulnerable to mechanical trauma, thermal burns, and microbial infections, which can cause corneal lesions. In mild cases, the transparency of the cornea will decrease and affect the patient's vision. In severe cases, it will cause corneal opacity and blindness. According to WHO statistics, there are currently nearly 60 million blind patients with corneal disease in the world, and nearly 5 million people in my ...

AI Technical Summary

Problems solved by technology

For example, the source of human-derived natural biological materials is limited, which cannot meet the needs of large-scale reconstruction of tissue-engineered corneas in vitro, and the tissue-engineered corneal carrier scaffolds made of natural biological materials such as heterogeneous (pig and other animal sources) corneal stroma after decellularization treatment have also been reported. There are many defects such as large structural damage, low transparency, and poor mechanical properties.

And some use detergents such as sodium lauryl sulfate, triton X-100 (Triton X-100) in the process of preparing acellular keratoma sheet, and some use neutral protease or pancreatin, and these materials are harmful to The action of corneal stromal slices is severe, which can destroy the collagen lamina and proteoglycans in the matrix, and may also cause inflammation after transplantation; while tissues made of natural biological macromolecules such as collagen, gelatin, and silk fibroin The engineered corneal carrier scaffold still has defects such as insufficient structural density, poor mechanical properties, and rapid degradation; the tissue engineered corneal carrier scaffold made of artificial synthetic materials has insufficient biocompatibility and high immunogenicity. shortcoming