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Aeromonas strain R1, preparation method of aeromonas strain and application of aeromonas strain in algae lysing and microcystin degradation

A technology of Aeromonas and bacterial strains, applied in the application field of algae-degrading microcystins, which can solve the problems of single function and less research

Active Publication Date: 2018-05-08
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most studies only focus on the single function of microorganisms to dissolve algae or degrade algae toxins, and there are few studies on the actual ability of algae dissolution or degradation of algae toxins in complex polluted water bodies, especially in the case of heavy metal pollution.

Method used

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  • Aeromonas strain R1, preparation method of aeromonas strain and application of aeromonas strain in algae lysing and microcystin degradation
  • Aeromonas strain R1, preparation method of aeromonas strain and application of aeromonas strain in algae lysing and microcystin degradation
  • Aeromonas strain R1, preparation method of aeromonas strain and application of aeromonas strain in algae lysing and microcystin degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Screening and Identification of Strain R1

[0044] S1: Centrifuge the rotten algae bloom collected from Jinghu Lake, Jinghu District, Wuhu City, Anhui Province, and collect the supernatant;

[0045] S2: Inoculate 10 mL of the supernatant into 90 mL of beef extract-peptone liquid medium under aseptic conditions, and shake at 180 r / min and 37°C for 24 hours to obtain the bacterial liquid;

[0046]S3: Inoculate 0.1 mL of the bacterial solution onto beef extract peptone solid medium containing 10 mg / L cadmium, and incubate at 37°C for 72 days;

[0047] S4: The well-growing colony was purified and inoculated into the slant medium, stored at 2-6°C until use, and named as R1.

[0048] In the step S4, the purification method is to pick the colony on the beef extract peptone solid medium, connect the cadmium-containing beef extract peptone medium by the streaking method, culture the plate upside down, and then pick the colony and continue to repeat the previous steps, until th...

Embodiment 2

[0052] Algae lysis test

[0053] A ring of R1 strain was inoculated into 200mL beef extract-peptone liquid medium, cultured at 180r / min and 37°C for 30h to obtain R1 strain, which had an absorbance value of 1.0 at 620nm. The preparation method of the R1 bacterial solution involved in the following examples is the same.

[0054] The preparation method of the Microcystis aeruginosa algae liquid is as follows: the purchased algae are inserted into the BG11 medium, and the algae are shaken every day, and cultivated in a light incubator at 25° C., light intensity 2500 lx, and light-dark ratio 12h:12h.

[0055] The algae-dissolving test was designed according to Table 1.

[0056] Table 1 algae-dissolving test

[0057]

[0058]

[0059] Each group of experiments was repeated three times, cultured at 25°C, light intensity 2500lx, and light-dark ratio 12h:12h, observed the morphological changes of Microcystis aeruginosa cells and measured the content of chlorophyll a in algae c...

Embodiment 3

[0061] Degradation Test of Algal Toxins by R1 Strain

[0062] Put the R1 bacterial solution cultivated for 24 hours into the M9 medium with microcystin as the substrate according to the volume ratio of 5%, the initial concentration of algae toxin is 1.84mg / L, and the concentration of cadmium is 0.1mg / L; another experimental group, The M9 medium only contains algae toxin, the concentration is 1.84mg / L, and a negative control group is set up. Cultivate in a shaker at 37°C and 180r / min for 7 days, take a sample every 24 hours, and detect changes in the content of algal toxins with a microcystin detection kit produced by Beacon. Detection method: pipette 50 μl of enzyme markers into the small wells of the microwell plate; pipette 50 μl of the standard sample, sample, and negative control into the corresponding microwells; add 50 μl of antibody solution to each space, and incubate the film for 30 minutes with rapid shaking; wash the microwell with the cleaning solution Wells were ...

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Abstract

The invention discloses an aeromonas strain R1, a preparation method of the aeromonas strain and the application of the aeromonas strain in algae lysing and microcystin degradation. The strain R1 is obtained by being screened from rotten algal bloom in the mirror lake, the strain is high in cadmium resistance, can be applied to algae lysing and can degrade microcystins, and through 16SrDNA sequence comparison, analysis and identification, the R1 belongs to aeromonas. The strain has good tolerance to Cd2+, and can still grow normally in a medium containing 50 mg / L Cd2+; the strain lyses microcystis aeruginosa by secreting extracellular substances, and the substances are resistant to high temperature and refer to non-nucleic-acid substances, polysaccharides and other substances. In the presence of 0.1 mg / L Cd2+, the algae lysing capacity of the strain is enhanced; when the initial concentration of microcystins is 1.84 mg / L, the degradation rate is 43.5% within seven days.

Description

technical field [0001] The invention relates to an Aeromonas bacterial strain R1, a preparation method and its application in algae-degrading microcystin degradation. Background technique [0002] In recent years, water bodies such as rivers and lakes in many cities have been polluted. Not only the nitrogen and phosphorus exceeded the standard, but the eutrophication of the water body became more and more serious, and the cyanobacteria bloomed frequently, producing many kinds of toxins, including the most dangerous microcystin, and a large amount of heavy metals remained, which affected human health. [0003] For this kind of compound polluted water body, single physical and chemical methods are difficult to repair or control, and may cause secondary pollution to the water body. Microbial removal of cyanobacteria and microcystins has the advantages of high efficiency, strong safety and environmental friendliness. The algalytic microorganisms found in previous studies includ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/22
CPCC02F3/34C02F2101/22C12N1/20C12N1/205C12R2001/01
Inventor 刘爱民刘宇卢存龙董德武宛曼
Owner ANHUI NORMAL UNIV
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