141 results about "Drug assay" patented technology

The present invention discloses a human bile duct cancer cell line and applications thereof, wherein the human bile duct cancer cell line is preserved in the China Center for Type Culture Collection, and has the preservation number of CCTCC NO:C201452. The human bile duct cancer cell line applications are the human bile duct cancer cell line is used for preparing the reagent for producing bile duct cancer in immunodeficient mammal. According to the present invention, the human bile duct cancer line has stable character, can be stably and repeatedly passaged, has tumorigenicity in animals, can successfully prepare bile duct cancer animal models, can be used for analyzing in vitro drug sensitivity and drug resistance and drug sensitivity and drug resistance of in vivo animal experiments so as to establish the in vitro and in vivo associated anti-tumor drug activity testing platform, is the ideal human primary bile duct cancer cell line used for basic research and preclinical application, and provides the ideal material for the further tumor occurrence mechanism research and drug testing.

The invention discloses preparation of an enzyme linked immunosorbent assay (ELISA) kit used for detecting dihydropyridine drug residues, and a detection method for detecting dihydropyridine drug residues. The enzyme linked immunosorbent assay kit is high in detection sensitivity, accuracy, speed, and specificity; operation is simple; and the detection method is suitable for large-scale sample detection. The enzyme linked immunosorbent assay kit comprises an enzyme labeled plate coated with dihydropyridine drug antigens, a dihydropyridine drug standard substance, a dihydropyridine drug antibody working solution, a dihydropyridine drug enzyme labeled second antibody working solution, a substrate solution A, a substrate solution B, a stopping solution, a concentrated sample diluent, and a concentrated washing liquid. Solid phase indirect competitive enzyme-linked immune response is taken as the principle of the enzyme linked immunosorbent assay kit used for detecting dihydropyridine drugs. According to the detection method, an extracted sample, the dihydropyridine drug enzyme labeled second antibody working solution, and the dihydropyridine drug antibody working solution are added into corresponding enzyme labeled holes for a certain time of incubation; after plate washing, the substrate solution A, and the substrate solution B are added, so that the color of an obtained mixture is changed to be blue under enzyme action; and the stopping solution is added, and the color is changed from blue to yellow. The shade of color developing and the content of dihydropyridine drugs in the standard substance or the sample are inversely related. The direction method can be used for directly detecting dihydropyridine drugs in health care products.

The invention discloses a method for detecting residue of sodium pentachlorophenate, which belongs to the technical field of drug detection. The method for detecting the residue of the sodium pentachlorophenate comprises the following steps: extracting residue of sodium pentachlorophenate in a sample by utilizing sulfuric acid and normal hexane, and then detecting by utilizing a high performance liquid chromatography-mass spectrometry, wherein the concentration of the sulfuric acid solution is 6 percent. The method has the beneficial effects that the method is suitable for detecting the residue of various matrix sodium pentachlorophenate, the used reagent is low in cost and small in toxicity, impurities can be effectively removed, and the operation steps can be simplified; and by adopting the high performance liquid chromatography-mass spectrometry, compared with the single high performance liquid chromatography, the method has the advantages of high sensitivity, good repetition and low detection limit.

This invention provides a system for modeling neurodegenerative and other diseases through somatic gene transfer. In addition, methods of multiple gene transfer, disease analysis and drug testing are provided for.

The invention provides a novel all-in-one sampling detector for DNA sample collection and saliva alcohol and drug detection. The all-in-one sampling detector consists of a sampling detection rod and adrug detection card, wherein the sampling detection rod is used for saliva and DNA sample collection and alcohol detection. According to the invention, a detection card which can be used for detection of a drug in a saliva sample is prepared based on colloidal gold competitive inhibition method immunochromatography. The all-in-one sampling detector provided by the invention is convenient to use and simple to operate, and can complete DNA sample collection and detection of three indicators of saliva alcohol content while completing drug saliva detection. This product is simple in structure andsmall in size, is suitable for on-site use during law enforcement, and is also suitable for individuals and families to use at any time; painless collection is realized without any trauma or invasiveness, so that a user does not feel any discomfort; the detector is disposable, and has no risk of cross-infection. The collected DNA sample is suitable for genetic analysis and detection and other medical examination.

The invention belongs to the technical field of drug detection, and particularly relates to a method for directly determining three degradation products of tryptophan in a compound amino acid injection. The three degradation products of tryptophan refer to dioxindole alanine, kynurenine and 2-hydroxytryptophan. The method comprises the following specific steps: (1) preparing the following chromatographic conditions of a liquid chromatograph: a chromatographic column being an octadecylsilane chemically bonded silica chromatographic column; a mobile phase comprising a mobile phase A being a phosphate buffer solution and a mobile phase B being acetonitrile; the flow rate being 0.95-1.05 mL / min; the column temperature being 25-35 DEG C; the sample size being 5-100 [mu]L; gradient elution; andthe detection wavelength being 254 nm; (2) preparing a test solution; (3) preparing a reference substance solution; and (4) determining. The method has the advantages of strong specificity, high sensitivity and good repeatability, solves the technical problems of interference of other amino acid components in the prescription, inaccurate determination result caused by derivative reaction in otherdetection methods, and the like, and ensures the product quality.

The invention provides a tubular tissue structure body and a constructing method thereof. The method comprises the following steps: A, preparing printing ink; B, culturing cells for printing in vitroto obtain a cell culture solution; and C, respectively connecting the printing ink and the cell culture solution with different spray heads of a biological printer, jointly printing the printing ink and the cell culture solution on a winding rod to form seamless tubular tissue, and then removing the winding rod to obtain the hollow tubular tissue structure body. According to the method, a tubularstructure with a controllable structure and cell / material components is formed through a horizontal winding type cell 3D printing technology. The tubular structure is close to a human vessel in shapeand size, has mechanical and biological properties meeting the requirements of a human body, has clinical application potential, and can be applied to the aspects of drug detection, tissue engineering, regenerative medicine, in-vitro physiological model / pathological model / pharmacological model construction, tissue / organ / human body chips and the like.