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Device and method for detecting beta-agonists drug

A technology of agonists and drugs, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem that the simultaneous detection of a class of drugs cannot be realized, and achieve the effect of saving detection costs and shortening the detection time

Inactive Publication Date: 2012-08-22
SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2002, the Ministry of Agriculture, the Ministry of Health, and the State Food and Drug Administration announced again that the addition of clenbuterol hydrochloride and ractopamine and other seven kinds of clenbuterol in feed and animal drinking water was explicitly prohibited, but in actual operation only Detection of clenbuterol hydrochloride, ractopamine, salbutamol, etc., which are not included in the routine detection
That is, one antibody detects one β-agonist drug, and it is impossible to realize the simultaneous detection of one class of drugs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] 1) Preparation of test strips

[0028] Preparation of the sample pad: cut the cellulose membrane into strips with a size of 1.5×30cm, and set aside;

[0029] Preparation of nitrocellulose membrane: Cut the nitrocellulose membrane into 2.5×30cm strips, spray β-agonist drug antibody / receptor antibody or β-agonist drug antibody / Receptor secondary antibody (quality control line), β-agonist drug ligand (detection line), and dry at room temperature;

[0030] Preparation of absorbent pad: Cut the absorbent paper into 3.5×30cm strips for later use;

[0031] Assembly of test strips: Paste absorbent pads, nitrocellulose membranes, and sample pads on the bottom plate in sequence, cut into 5mm-wide test strips, and store in a dry place at 2-8°C.

[0032] 2) Preparation of reaction cup

[0033] According to the amount of 100 μL / well, add colloidal gold-labeled β-agonist drug antibody / receptor to the 96-well microplate;

[0034] Freeze at -80°C for 24 hours;

[0035] colloidal ...

example 2

[0041] Example 2 Immunochromatography detection device (test strip mode)

[0042] 1) Preparation of test strips

[0043] Preparation of the sample pad: cut the cellulose membrane into strips with a size of 1.5×30cm, and set aside;

[0044] Preparation of binding pad: Colloidal gold-labeled β-agonist drug antibody / receptor is sprayed on glass fiber paper, vacuum-dried, and set aside.

[0045] Preparation of nitrocellulose membrane: Cut the nitrocellulose membrane into 2.5×30cm strips, spray β-agonist drug antibody / receptor antibody, β-agonist drug ligand on different positions of the NC membrane (Detection line), dry at room temperature;

[0046] Preparation of absorbent pad: Cut the absorbent paper into 3.5×30cm strips for later use;

[0047]Assembly of test strips: Paste absorbent pads, marker binding pads, nitrocellulose membranes, and sample pads on the bottom plate in sequence, cut into 5mm-wide test strips, and store them dry at 2-8°C.

[0048] 2) Detection of β-agoni...

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PUM

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Abstract

The invention relates to the field of biological detection and discloses a detection device and a detection method of a large class of beta-agonists drugs by applying an immune chromatography technology. According to the invention, an antibody / receptor which is reacted with a large class of the beta-agonists drugs is used for preparing an immune chromatography test strip to realize the simultaneous detection of a large class of the beta-agonists drugs. Compared with the prior art, the detection on a large class of the beta-agonists drugs for one time can be realized, the detection cost is low and the method is convenient and fast.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a detection method and a device for detecting beta-agonist drugs using immunochromatography technology. Background technique [0002] β-agonists, also known as β-stimulants, are a class of synthetic drugs, mainly used to prevent and treat bronchial asthma and bronchospasm in humans and animals, and are called β-adrenergic stimulants in medicine. This kind of medicine is added to the feed and drinking water of livestock and poultry to promote the growth of lean meat and inhibit the growth of fat meat, commonly known as clenbuterol. Because such drugs will accumulate in the liver, lungs and other internal organs, the eaters are prone to poisoning symptoms such as nausea, dizziness, muscle tremors, and palpitations, which seriously affect the health of consumers and even threaten people's lives. Countries such as Europe and the United States have already legislated to prohibit t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
Inventor 朱海付辉林季敏李颖王西丽李金峰蒋原薛峰
Owner SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
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