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Method for detecting residual quantity of amoxicillin and clavulanic acid in tissues

A technology of amoxicillin and clavulanic acid, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of lack of drug detection methods

Inactive Publication Date: 2020-03-27
内蒙古联邦动保药品有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the maximum residue limits of amoxicillin and clavulanic acid in animal tissues (including but not limited to muscle, liver, kidney and fat) have been regulated accordingly, but how to establish a fast, accurate, effective and Robust methods for the determination of amoxicillin and clavulanic acid residues are yet to be developed, and methods for the detection of both drugs in fat are still lacking

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  • Method for detecting residual quantity of amoxicillin and clavulanic acid in tissues
  • Method for detecting residual quantity of amoxicillin and clavulanic acid in tissues
  • Method for detecting residual quantity of amoxicillin and clavulanic acid in tissues

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Embodiment

[0082] 1. Materials

[0083] 1.1 Reagents and medicines

[0084] Test drug: amoxicillin sodium and potassium clavulanate for injection, specifications (amoxicillin sodium: potassium clavulanate, 0.6g: 0.15g), batch number: 161017, provided by Inner Mongolia Federal Animal Health Pharmaceutical Co., Ltd.

[0085] Amoxicillin reference substance: (batch number: 130409-201512, content ≥ 86.90%) was purchased from China National Institutes for Food and Drug Control, and stored in the dark at 4°C;

[0086] Clavulanic acid reference substance: (batch number: 130429-201307, content ≥ 95.00%) was purchased from China National Institutes for Food and Drug Control, and stored at 4°C in the dark;

[0087] Methanol: chromatographic grade pure, American MERCK company, batch number: 10894707725;

[0088] Acetonitrile: chromatographic grade pure, American MERCK company, batch number: JA055130;

[0089] Formic acid: liquid phase mass spectrometry grade, American Honeywell Company, batch nu...

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Abstract

The invention relates to the field of drug detection, in particular to a method for detecting the residual quantity of amoxicillin and clavulanic acid in tissues, which comprises the following steps:1) preparing an amoxicillin and clavulanic acid reference substance solution and a to-be-detected tissue extract sample solution; 2) carrying out high performance liquid chromatography-tandem mass spectrometry detection, and quantifying by an external standard method or qualifying by a contrast method; wherein the chromatographic conditions are as follows: a chromatographic column is ACQUITYHSS T3, a mobile phase A is acetonitrile, and a mobile phase B is 0.1% formic acid water; wherein the mass spectrum conditions of amoxicillin detection comprise multi-reaction monitoring, positive ion scanning and capillary voltage of 3kV; mass spectrum conditions of clavulanic acid detection include multi-reaction monitoring, negative ion scanning and capillary voltage of-3kV. The method has good sensitivity, accuracy and precision, and qualitative and quantitative detection of amoxicillin and clavulanic acid residues is effectively realized.

Description

technical field [0001] The invention relates to the field of drug detection, in particular to a method for detecting residues of amoxicillin and clavulanic acid in tissues. Background technique [0002] Amoxicillin (Amoxicillin) belongs to β-lactam antibiotics, is a semi-synthetic acid-resistant broad-spectrum penicillin antibiotics, also known as amoxicillin. The drug has a wide antibacterial spectrum, strong bactericidal power, and rapid action. Proteus, etc. are sensitive, and are used in various clinical bacterial infection diseases. Clavulanic acid (Clavulanic acid, also known as clavulanic acid), was independently discovered by Brown et al. and Napier et al. in 1976 and 1981 respectively. Its structure is very similar to the mother nucleus of penicillin. It has a good inhibitory effect on the β-lactamase produced by Staphylococcus aureus and many Gram-negative bacteria. It is a "suicide" inhibitor of the natural lactamase produced by bacteria. . Amoxicillin is acid...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/047G01N2030/062
Inventor 刘红池闫旭李德乐
Owner 内蒙古联邦动保药品有限公司
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