Human bile duct cancer cell line and applications thereof

A technology for cancer cells and cholangiocarcinoma, applied in the field of cell lines, can solve the problems of low biodiversity and large differences in biological characteristics of cholangiocarcinoma, and achieve the effect of stable characteristics

Active Publication Date: 2016-01-20
SHANGHAI LIDE BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a new human cholangiocarcinoma cell line in view of the technical problem that the existing human cholangiocarcinoma cell lines have low biodiversity and are quite different from the clinical biological characteristics of cholangiocarcinoma. systems and their applications

Method used

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  • Human bile duct cancer cell line and applications thereof
  • Human bile duct cancer cell line and applications thereof
  • Human bile duct cancer cell line and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1L

[0043] Example 1 Establishment of LIPF155C cell line

[0044] SCID mice: 5 SCID mice, female, weighing 16.0±1.0 g, aged 5 weeks, raised in SPF environment. SCID mice were provided by Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd.

[0045] A fresh excision specimen of clinical cholangiocarcinoma (female, 79 years old, primary cholangiocarcinoma, nationality: Han, native of Anhui) was obtained from Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine. The pathological diagnosis was: intrahepatic cholangiocarcinoma, low medium differentiation), immediately immersed in pre-chilled sterile HBSS buffer (containing 500 U / mL penicillin G, 500 μg / mL streptomycin sulfate, and 1.25 μg / mL amphotericin B). In a biological safety cabinet, the specimens were washed with fresh sterile HBSS buffer, cut into small pieces of 20-50 mg, and inoculated subcutaneously in the back of the armpit of SCID mice. After 20 days of inoculation, it was found by touch t...

Embodiment 2L

[0049] Example 2 Biological characteristics and application of LIPF155C cells

[0050] In the present invention, the RPMI1640 medium containing fetal bovine serum is used to culture the LIPF155C cells obtained in Example 1, so that they can grow in vitro for a long time and be stably passaged. When the cells were passed to more than 15 generations, the cell characters gradually stabilized, and related biology, genetics and tissue source identification were carried out, and the cells had the same stable characters until the 30th generation. The experimental observation and verification showed that the LIPF155C cells grown in vitro were epithelial-like. Under the inverted microscope, the cells grew adherently, uniformly dispersed, varied in size, irregular polygonal in shape, lost contact growth inhibition, and showed malignant growth. The LIPF155C cells can form tumors in nude mice and have tumorigenicity. The LIPF155C cells form a corresponding relationship with the clinical ...

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Abstract

The present invention discloses a human bile duct cancer cell line and applications thereof, wherein the human bile duct cancer cell line is preserved in the China Center for Type Culture Collection, and has the preservation number of CCTCC NO:C201452. The human bile duct cancer cell line applications are the human bile duct cancer cell line is used for preparing the reagent for producing bile duct cancer in immunodeficient mammal. According to the present invention, the human bile duct cancer line has stable character, can be stably and repeatedly passaged, has tumorigenicity in animals, can successfully prepare bile duct cancer animal models, can be used for analyzing in vitro drug sensitivity and drug resistance and drug sensitivity and drug resistance of in vivo animal experiments so as to establish the in vitro and in vivo associated anti-tumor drug activity testing platform, is the ideal human primary bile duct cancer cell line used for basic research and preclinical application, and provides the ideal material for the further tumor occurrence mechanism research and drug testing.

Description

technical field [0001] The present invention relates to the field of cell lines, in particular to a human cholangiocarcinoma cell line and its application. Background technique [0002] Primary cholangiocarcinoma is a type of tumor originating from epithelial cells with late diagnosis and poor prognosis. Due to the lack of specific clinical manifestations and diagnostic indicators in the early stage, most of the primary cholangiocarcinomas found clinically are intermediate and advanced cases. Cholangiocarcinoma accounts for about 3% of gastrointestinal malignancies. The average age of onset is about 50 years, and the incidence in men is about 1.5 times that in women. Asians are twice as likely as blacks and whites. In the past three decades, the incidence of cholangiocarcinoma has been increasing worldwide, but its pathogenesis remains unclear. Risk factors for cholangiocarcinoma are known to be related to chronic inflammation of the bile duct, viral infection, congenita...

Claims

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Application Information

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IPC IPC(8): C12N5/09C12Q1/02A01K67/027A61K49/00C12R1/91
Inventor 王科欧阳可栋秦宵然赵刚闻丹忆张伟朱纯超
Owner SHANGHAI LIDE BIOTECH CO LTD
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