Preparation of enzyme linked immunosorbent assay kit used for detecting dihydropyridine drug residues

Abstract

The invention discloses preparation of an enzyme linked immunosorbent assay (ELISA) kit used for detecting dihydropyridine drug residues, and a detection method for detecting dihydropyridine drug residues. The enzyme linked immunosorbent assay kit is high in detection sensitivity, accuracy, speed, and specificity; operation is simple; and the detection method is suitable for large-scale sample detection. The enzyme linked immunosorbent assay kit comprises an enzyme labeled plate coated with dihydropyridine drug antigens, a dihydropyridine drug standard substance, a dihydropyridine drug antibody working solution, a dihydropyridine drug enzyme labeled second antibody working solution, a substrate solution A, a substrate solution B, a stopping solution, a concentrated sample diluent, and a concentrated washing liquid. Solid phase indirect competitive enzyme-linked immune response is taken as the principle of the enzyme linked immunosorbent assay kit used for detecting dihydropyridine drugs. According to the detection method, an extracted sample, the dihydropyridine drug enzyme labeled second antibody working solution, and the dihydropyridine drug antibody working solution are added into corresponding enzyme labeled holes for a certain time of incubation; after plate washing, the substrate solution A, and the substrate solution B are added, so that the color of an obtained mixture is changed to be blue under enzyme action; and the stopping solution is added, and the color is changed from blue to yellow. The shade of color developing and the content of dihydropyridine drugs in the standard substance or the sample are inversely related. The direction method can be used for directly detecting dihydropyridine drugs in health care products.

Description

technical field [0001] The invention relates to the technical field of enzyme-linked immunoassay detection, in particular to an enzyme-linked immunoassay kit for detecting dihydropyridine drugs. Background technique [0002] Dihydropyridines are a class of calcium antagonists with obvious dilation of blood vessels, and dihydropyridine calcium antagonists are cardiovascular drugs widely used in clinical practice. Long-term use and effective control of blood pressure, less likely to cause reflex tachycardia or orthostatic hypotension. For hypertensive patients with angina pectoris, hyperlipidemia, arrhythmia, and migraine, dihydropyridine calcium antagonists can be used as the drug of choice. It is the first-line drug for various degrees of hypertension, angina pectoris and arrhythmia. Compared with other types of drugs, it has an absolute advantage in its exact curative effect, wide range of effects and relatively low price. However, if it is added to health care products,...

AI Technical Summary

Problems solved by technology

However, if it is added to health care products, it will cause corresponding adverse reactions to normal people and endanger health.

[0003] At present, the determination methods of dihydropyridines mainly include high-performance liquid chromatography, gas chromatography, thin-layer chromatography, high-performance liquid chromatography-mass spectrometry, etc. The detection results are accurate and reliable, but the equipment is relatively expensive. And the sample pretreatment process is relatively complicated

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