Method for determining various types of abuse drugs in human whole blood

Abstract

The invention belongs to the technical field of drug detection and judicial identification, and in particular relates to the detection of seven abused drugs amphetamine, methamphetamine, methylenedioxyamphetamine, methylenedioxy The method for extracting and measuring methamphetamine, ketamine, pethidine and methadone uses methanol as a dispersant and a protein precipitation solvent, uses dichloromethane as an extraction solvent, and forms a microemulsion system of a three-solvent system in an aqueous solution of pH 13, With the assistance of ultrasound, it is further emulsified for liquid-liquid microextraction, and the extraction process does not need to evaporate the extraction solvent; this method has high enrichment multiple and recovery rate, simple and fast operation, high sensitivity, strong specificity, wide linear range and It has the advantages of low cost and environmental friendliness, and can meet the characteristics of urgent judicial appraisal tasks and high requirements for detection time. The detection system of the invention provides a new technical platform for drug detection.

Description

technical field [0001] The invention belongs to the technical field of drug detection and judicial identification, and relates to a method for measuring drugs in human whole blood, in particular to ultrasonic-assisted dispersive liquid-liquid microextraction (ultrasound-assisted dispersive liquid-liquid microextraction, UA-DLLME) for human whole blood This method has the advantages of high enrichment multiple and recovery rate, simple and fast operation, low cost, and environmental friendliness. Background technique [0002] According to reports, amphetamine (amphetamine, AMP), methamphetamine (methamphetamine, MAMP), methylenedioxyamphetamine (methylenedioxyamphetamine, MDA), methylenedioxymethamphetamine (methylenedioxymethamphetamine, MDMA), ketamine (ketamine), Pethidine (pethidine) and methadone (methadone) are drugs or drugs widely abused; AMP, MAMP, MDA and MDMA are all amphetamine-type stimulants, which can excite the central nervous system. Mainly, the main compone...

AI Technical Summary

Problems solved by technology

Practice has shown that although ELISA has no special requirements for sample pretreatment, its specificity is poor, it is difficult to distinguish structural analogs, and sometimes even false positives occur, so this method is often only used as an initial method. The means of sieving; using gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-mass spectrometry (LC-MS) has high specificity and sensitivity, and can further confirm the compound. -MS or LC-MS often need to extract and purify the sample before analyzing the biological sample, but the commonly used extraction method is mainly to use a large volume of organic solvents, such as ether, n-hexane, etc., so this method is used The extraction method often involves the need to evaporate the extract to achieve the effect of enrichment, but existing studies have shown that AMP and MAMP will evaporate rapidly with the evaporation of the extract, and the reproducibility and standard of analysis The linearity of the curve will be affected, making it difficult to accurately quantify the two substances described in the biological sample; although it has been reported that AMP can be avoided by adding acid or evaporating the sample under low temperature conditions or performing derivatization operations and the volatilization of MAMP, but these sample pretreatment methods are relatively cumbersome and time-consuming, and the amount of sample used is large, and the amount of organic solvent used is not friendly to the environment. The sampling volume has a large requirement

Images