214 results about "Chemogenetics" patented technology

The invention relates to a control method of the fingerprint spectrum quality of cordyceps sinensis powder raw material in botanical drug for strengthening vital qi and removing blood stasis, comprising the steps that: (1) cordyceps sinensis powder is extracted: 0.100g of cordyceps sinensis powder is taken, purified water is added, the ultrasonic extraction, the filtration and the sample injection are carried out; (2) the gradient elution with mobile phase is carried out: octadecyl silane bonded silica gel is taken as a filler, water and acetonitrile are taken as mobile phase to carry out the gradient elution for 0 to 30min and 0 to 7 percent B; (3) a standard fingerprint spectrum is established: the HPLC standard fingerprint spectrum of the cordyceps sinensis powder is determined, and 3 characteristic peaks are selected; (4) the quality control of the fingerprint spectrum is carried out: the relative retention time of No.2 peak uridine, No.3 peak guanosine and No.4 peak adenosine are 0.44 plus or minus 0.03, 0.68 plus or minus 0.03 and 1.00 respectively; the HPLC fingerprint spectrum of the sample is compared with the contrast fingerprint spectrum. The similarity calculated by the 5 common peaks is not less than 0.9, (5) the preparation of the cordyceps sinensis powder raw material is carried out; the control method has good repetitivity and can fully reflect the basic characteristics of nucleoside ingredients of the cordyceps sinensis powder.

The invention discloses a construction method for ion chromatography fingerprint spectrums of ganoderma lucidum spore powder polysaccharide, which comprises acid-enzyme partial hydrolysis of the ganoderma lucidum spore powder polysaccharide, the ion chromatography fingerprint analysis of monosaccharide components and oligosaccharide components of hydrolysis products of the ganoderma lucidum spore powder polysaccharide and the confirmation of standard fingerprint spectrums. By means of the high performance anion-exchange chromatography (HPAEC) analysis and the comparison of the fingerprint spectrums of polysaccharide contents in ganoderma lucidum spore powder samples from 20 different production places, the common fingerprint characteristics of the polysaccharide contents in the ganoderma lucidum spore powder samples are determined, the standard fingerprint spectrums of the monosaccharide components and the oligosaccharide components are obtained respectively, and 11 monosaccharide common characteristic peaks in monosaccharide spectrums, 4 oligosaccharide common peaks in the oligosaccharide spectrum and 1 polysaccharide peak are determined. According to the construction method for the ion chromatography fingerprint spectrums of the ganoderma lucidum spore powder polysaccharide, the method is stable, the precision degree is high, the reproducibility is good, the method is easy to master, quality conditions and production places of the ganoderma lucidum spore powder polysaccharide can be grasped from two aspects of fingerprint spectrums of the monosaccharide components and the oligosaccharide components of the acid-enzyme partial hydrolysis products which can reflect the components and structural characteristics of the ganoderma lucidum spore powder polysaccharide, and a new scientific method is provided for the quality control and the authenticity identification of the ganoderma lucidum spore powders.

The invention relates to a method for semi-quantitatively determining chloride, bromide and iodine ions by indicator displacement reaction. The method comprises chelating an indicator with a metal ion, and replacing the metal ion from the chelate by chloride, bromide or iodine ions through precipitation reaction of the chloride, bromide or iodine ions to the metal ion, so that the chelate returns to the initial color of the indicator. In the method, color imaging apparatuses (scanner, digital camera, etc.) are used for collecting the color change of the indicator; and a fingerprint spectrum of corresponding concentration of chloride, bromide or iodine ions is constructed according to the red, green and blue spectral data corresponding to the color change. In the sample testing process, a mathematical statistical method, such as clustering analysis or principal component analysis, is adopted to compare the sample spectrum with the fingerprint spectrum database, so as to carry out semi-quantitatively analysis on unknown concentrations of chloride, bromide or iodine ions.

The related detection method for amino acid content in Xiasangju preparation comprises: (a) preparing reference solution; (b) preparing sample solution; (c) chromatogram condition: using octadecylsilicane chemically bonded silica as filler, using the gradient elution liquid with 0.05~0.10mol/L NaAc solution buffer and 20-80% acetonitrile solution as mobile phase with pH value as 4.50-5.05, 30-50Deg temperature, activation wavelength 250nm, emission wavelength 395nm, flow rate as 0.5-1.5mL .min-1, and 30-80min; (d) obtaining the fingerprint spectrum with HPLC. This invention is simple and stable, and has well repeatability and precision.

The invention relates to a gentiana macrophylla capsule fingerprint spectrum and an application of the spectrum to quality control and component analysis. According to the spectrum, a HPLC (high-performance liquid chromatography) fingerprint spectrum common mode of a gentiana macrophylla capsule is determined by the aid of a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system (2004A), 23 common peaks are calibrated, similarity is 0.966-0.982, and the content of loganic acid, shanzhiside methyl ester, 6'-O-beta-D-glucose gentiopicroside, swertiamarin,gentiopicroside, sweroside, isoorientin and isovitexin in 10 preparations is measured. According to the spectrum, a HPLC fingerprint spectrum method of the gentiana macrophylla capsule and a content measuring method are built, the method has good analysis evaluation ability and the advantages of accuracy, convenience, stability, reliability and the like, so that the method can serve as an effective evaluation method for the quality of the preparation.

The invention relates to a Tibetan picroside extract with a definite spectrum-activity relationship, in particular to a Tibetan picroside extract contained with picroside I (C24H28O11) and picroside II (C23H28O13), and the sum of the contents of the picroside I (C24H28O11) and the picroside II (C23H28O13) is not lower than 50 percent; and on the fingerprint for the extract, the peak area ratio between the picroside II and the picroside I is 1.30 to 2.40 calculated according to the total picroside chromatogram. The extract has the remarkable action of liver injury prevention and has good application prospect in the field of liver injury prevention medication.

The invention relates to an establishing method of rhizoma anemarrhenae HPLC-ELSD (High Performance Liquid Chromatography-Evaporative Light Scattering Detector) fingerprint and a standard fingerprint established by using the establishing method. The establishing method comprises chromatographic conditions, the preparation of a test substance solution, and the preparation of a control substance solution, wherein the chromatographic condition is a Thermo C8 chromatographic column (4.6mm*250mm, 5 microns); a mobile phase is an acetonitrile-0.2% acetic acid aqueous solution for gradient elution; the column temperature is 20 DEG C; the flow speed is 1mL.min<-1>; the flow speed of an ELSD atomizer is 2.6L.min<-1>; the temperature of a drift tube is 100 DEG C; and the sampling size is 20 microlitres. The establishing method provided by the invention has the advantages of good stability and repeatability, high precision, capability of simultaneous reflection of the characteristics of saponins and xanthones, which are the main chemical ingredients of the rhizoma anemarrhenae, and capability of reflecting the quality of the rhizoma anemarrhenae according to the overall characteristics of the chromatogram; and the establishing method can be used as one method for controlling the quality of the rhizoma anemarrhenae.

The invention provides methods for establishing fingerprints of cistanche extracts and identifying quality of drugs, comprising preparation of test solution, facture of fingerprints, determination of standard fingerprints and identification of cistanche drugs. HPLC fingerprints are established for methanol extracts of the cistanche drugs with different places of production, different quality or different storage time, and 21 common characteristic peaks are determined through analysis and comparison and form the fingerprint characteristics of the cistanche drugs, and the fingerprint characteristics can be used as the standard fingerprints of the cistanche drugs. The fingerprints of the cistanche drugs needing to be identified can be compared with the standard fingerprints and the common characteristic peaks are detected to identify the quality of the cistanche drugs. The methods are characterized by simpleness and convenience, good reproducibility, multiple characteristic peaks, accuracy, reliability and the like, and ensure the quality control of the cistanche drugs to be more perfect and scientific.

The invention relates to folium artemisiae argyi extracts, methods and application and particularly relates to a folium artemisiae argyi extract extracted by ethyl acetate and preparation and detection methods and application thereof. Effective ingredients of the folium artemisiae argyi extract consist of flavonoids and sterols. The invention provides application of the folium artemisiae argyi extract in the preparation of anti-tumor drugs, and provides an analysis method for constructing a folium artemisiae argyi extract fingerprint by using an ultrahigh performance liquid chromatography-mass spectrometry (UPLC-MS) analysis technology and analysis results. The method comprises the step of acquiring the UPLC-MS analysis conditions for the folium artemisiae argyi extract, features of chromatographic peaks, compound structure information provided by mass spectrums of chromatographic components, and the like. The fingerprint and technology thereof disclosed by the invention can be applied to the species identification of folium artemisiae argyi and the quality monitoring on the folium artemisiae argyi extracts.

This invention provides a non-invasion for testing liver cancer, which sets up a liver cancer protein set fingerprint atlas model used in the serum test. Said model is composed of a HAS set mass spectrum atlas and an artificial nerve net analysis method used in early serology test. The atlas is composed of the protein with five M/Z. The method for setting up the atlas model includes: 1, preparation of serum, 2, mass spectrum test and collection of data for the serum specimen, 3, artificial nerve net analysis of the spectrum data, which can test the liver cancer before there is not any pathologic change appearing to the cell.

The invention provides a fingerprint spectrum detection method for a low-sugar intensified loquat distillate, relating to the technical field of drug detection. The fingerprint spectrum detection method comprises the following steps: (1) establishing a component standard characteristic spectrum of the low-sugar intensified loquat distillate; (2) determining a sample characteristic spectrum of a to-be-detected low-sugar intensified loquat distillate by virtue of the same method; and (3) making comparison on the sample characteristic spectrum of the to-be-detected low-sugar intensified loquat distillate and a standard characteristic spectrum of the low-sugar intensified loquat distillate, and judging the quality and authenticity of the low-sugar intensified loquat distillate. The fingerprint spectrum detection method has the beneficial effects that the integrality, the macroscopic characteristic and the fuzziness are realized; the problem that the real adding situation of a product is difficulty reflected by the content determination of a single component is overcome; a novel method and measures are provided for the complete and accurate evaluation of the quality of the low-sugar intensified loquat distillate; the similarity and difference of spectra are visually identified; and the authenticity and quality level of the sample are quantized by virtue of semi-quantitative indexes.

The invention provides an establishment method of a baphicacanthus cusia HPLC fingerprint. The method includes following steps: preparing a to-be-tested sample solution, determining chromatographic conditions, and performing detection with an HPLC instrument to obtain a HPLC fingerprint of a baphicacanthus cusia medicine and extracts thereof. Overall chemical information of the baphicacanthus cusia can be comprehensively expressed and fingerprint characteristics of the baphicacanthus cusia can be determined. The fingerprint is simple in method, is good in repeatability, is many in characteristic peaks and is accurate and reliable. An established HPLC fingerprint can effectively controls quality of the baphicacanthus cusia medicine.

The invention discloses a cold-coagulation-blood-stasis-syndrome-resistant differential metabolite metabolic pathway and study method of a Chinese angelica-based cold-coagulation blood-stasis treatment decoction. The method comprises: detecting and analyzing endogenous metabolites changed at different time points after cold-coagulation blood-stasis symptoms forming of a female rat under an ice-water bath and epinephrine induction and Chinese angelica-based cold-coagulation blood-stasis treatment decoction intervention by using an ultra-high performance liquid chromatography tandem mass spectrometry so as to obtain a fingerprint spectrum; with a multivariate variable statistical analysis method, carrying out screening from a plurality of variables and identifying 21 significantly changed metabolites; and enriching eight metabolic pathways related to different development stages of the cold-coagulation blood-stasis symptom by using ametaboanalyst open-source online metabonomics analysiswebsite. According to the invention, the related metabolic changes in the occurrence and development process of the old-coagulation blood-stasis symptoms and the treatment process of the Chinese angelica-based cold-coagulation blood-stasis treatment decoction are studied by using a dynamic analysis method; the disease occurrence and development mechanisms at different time points can be revealed;and the abnormal metabolic network regulated and controlled by medicines in the treatment process can be clarified.