Quality detection method for tripterygii hypoglauci medicinal materials

A quality detection method, the technology of Begonia medicinal materials, applied in the direction of measuring device, material separation, analysis of materials, etc., can solve problems such as lack of quality detection methods

Active Publication Date: 2013-04-10
GUANGZHOU CHEN LI JI PHARMA FACTORY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The quality testing methods for Kunming Begonia medicinal materials are limited to simple traits and microscopic identification, without qualitative or quantitative identification of the main active ingredients, and the lack of quality testing methods

Method used

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  • Quality detection method for tripterygii hypoglauci medicinal materials
  • Quality detection method for tripterygii hypoglauci medicinal materials
  • Quality detection method for tripterygii hypoglauci medicinal materials

Examples

Experimental program
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Effect test

experiment example 1

[0060] Experimental Example 1: Methodological Experiment

[0061] 1. Selection of extraction solvent

[0062] Get 1g of Kunming mountain crabapple medicinal powder, add 80ml each of ethyl acetate and methanol respectively, measure according to the method described in Example 1 of the present invention, the results show that the ethyl acetate extract has fewer impurities than methanol, and the fat-soluble components are extracted except for No. 32 peak Except for methanol, the other components such as tripterine and triptolide A are equivalent, while the water-soluble components are fully extracted by methanol. After weighing the pros and cons, methanol is finally selected as the extraction solvent. See attached Figure 1 and two .

[0063] 2. Comparison of extraction times

[0064] Take 1g of Kunming mountain crabapple medicinal material powder, extract it with methanol three times, 80ml each time, and measure according to the law. The result shows that one extraction can...

experiment example 2

[0067] Experimental Example 2: Stability Test

[0068] Take the test solution, inject samples at different time points of 0, 1.5, 3, 4.5, 6, 7.5, 24, 48, and 72 hours, and import the obtained chromatogram into the similarity calculation software to calculate the cosine similarity of the included angle. The similarities of the points are all above 0.99, and the spectra are consistent, indicating that the stability is good, see the attached Figure five .

experiment example 3

[0069] Experimental Example 3: Repeatability Test

[0070] Take Kunming mountain crabapple medicinal material powder, weigh five copies, measure according to the method described in Example 1 of the present invention, import the obtained chromatogram into the similarity calculation software, and calculate the cosine similarity of the included angle, the results are all above 0.99, and the 5 samples The spectra are consistent, indicating good repeatability, see appendix Figure six .

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Abstract

The invention aims to disclose a quality detection method for a fingerprint spectrum of tripterygii hypoglauci medicinal materials. An acetonitrile-methyl alcohol-0.02% of phosphoric acid solution is used as a moving phase; gradient elution is carried out; and the injection temperature is 20 DEG C. With the adoption of the fingerprint spectrum provided by the invention, not only can the content of triptolide, tripterine and wilforlide be determined, but also the microcosmic components of the tripterygii hypoglauci medicinal materials are analyzed, and a common mode of the fingerprint spectrum is formulated, so that the most advanced level can be reached in qualitative and quantitative aspects.

Description

technical field [0001] The invention relates to a quality detection method of traditional Chinese medicinal materials, in particular to a fingerprint quality detection method of Kunming mountain crabapple medicinal materials. Background technique [0002] The use of chromatographic fingerprints in the quality detection of traditional Chinese medicines is in line with the complex multi-component characteristics of traditional Chinese medicines. In some cases where the chemical structure is not clear, it can comprehensively control the stability of Chinese medicinal materials. Compared with traditional identification, fingerprints can better reflect the overall quality information of traditional Chinese medicine (plant medicine), and it is convenient and easy to analyze with the help of modern analytical instruments and other advanced means. [0003] The US FDA and WHO have passed the regulations that "fingerprints are allowed to determine the consistency of samples between ba...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 莫国强谢培山郭忠慧颜玉贞何风雷谢琳
Owner GUANGZHOU CHEN LI JI PHARMA FACTORY
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