Cold-coagulation-blood-stasis-syndrome-resistant differential metabolite metabolic pathway and study method of Chinese angelica-based cold-coagulation blood-stasis treatment decoction

Abstract

The invention discloses a cold-coagulation-blood-stasis-syndrome-resistant differential metabolite metabolic pathway and study method of a Chinese angelica-based cold-coagulation blood-stasis treatment decoction. The method comprises: detecting and analyzing endogenous metabolites changed at different time points after cold-coagulation blood-stasis symptoms forming of a female rat under an ice-water bath and epinephrine induction and Chinese angelica-based cold-coagulation blood-stasis treatment decoction intervention by using an ultra-high performance liquid chromatography tandem mass spectrometry so as to obtain a fingerprint spectrum; with a multivariate variable statistical analysis method, carrying out screening from a plurality of variables and identifying 21 significantly changed metabolites; and enriching eight metabolic pathways related to different development stages of the cold-coagulation blood-stasis symptom by using ametaboanalyst open-source online metabonomics analysiswebsite. According to the invention, the related metabolic changes in the occurrence and development process of the old-coagulation blood-stasis symptoms and the treatment process of the Chinese angelica-based cold-coagulation blood-stasis treatment decoction are studied by using a dynamic analysis method; the disease occurrence and development mechanisms at different time points can be revealed;and the abnormal metabolic network regulated and controlled by medicines in the treatment process can be clarified.

Description

technical field [0001] The invention relates to the technical field of medical analysis. More specifically, the present invention relates to Danggui Sini Decoction differential metabolite metabolic pathways and research methods for anti-cold coagulation and blood stasis syndrome. Background technique [0002] Metabonomics has the characteristics of "whole-dynamic-comprehensive", which is consistent with the pathogenesis of complex diseases and the onset characteristics of traditional Chinese medicine. Metabolomics is a new technology developed in recent years for qualitative and quantitative analysis of dynamic metabolite changes in biological systems. The change. The metabolomics research process usually includes biological sample collection, pretreatment, sample analysis, data processing, identification of markers, and interpretation of biological significance. Its technologies mainly include nuclear magnetic resonance (NMR), gas chromatography-mass spectrometry (GS / MS)...

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Problems solved by technology

Since the development of BSS is a slow and dynamic process, the intervention mechanism of DSD in the development of BSS has not been studied so far

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