Method for detecting fingerprint spectrum of Shengbai Koufuye

A technology of Shengbai oral liquid and standard fingerprint, applied in the field of fingerprint detection of Shengbai oral liquid, can solve problems such as insufficient solution to multi-component complex system of traditional Chinese medicine preparations

Active Publication Date: 2020-12-01
HUBEI MONYAN PHARMA CO LTD
View PDF7 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both national standards and patents above only disclose the physical and chemical identification and content determination of some active ingredients. The methods in the prior art are not enough to solve the problem of multi-component complex system of traditional Chinese medicine preparations. Therefore, the fingerprint of Shengbai Oral Liquid is established The map will be able to more comprehensively reflect the types and quantities of chemical components contained in traditional Chinese medicine and its preparations, and then describe, control and evaluate the quality of medicines as a whole

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting fingerprint spectrum of Shengbai Koufuye
  • Method for detecting fingerprint spectrum of Shengbai Koufuye
  • Method for detecting fingerprint spectrum of Shengbai Koufuye

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] The preparation of embodiment 1 Shengbai oral liquid

[0107] Step (1) consists of 240g of epimedium, 120g of psoraleae, 80g of aconite (made), 240g of medlar, 240g of astragalus, 240g of Caulis Spatholobus, 240g of madder, 120g of angelica, 240g of reed root, 120g of Radix Ophiopogon japonicus and licorice 120g spare;

[0108] Step (2) First put in the prescription amount of Shiwei decoction pieces (excluding Epimedium), add water, heat and decoct until boiling, then add the prescription amount of Epimedium, continue heating and boiling until boiling, extract for 1 hour, filter, Obtain one extraction filtrate, add water to the medicinal dregs and extract once again, filter to obtain the second extraction filtrate, combine the two filtrates, and concentrate the filtrate to extract I with a relative density of 1.24-1.27 (25 ± 5°C);

[0109] Step (3) slowly add 90% ethanol according to extract I: ethanol=1:1~1.2 in extract I, stir while adding, extract extract is dispers...

Embodiment 2

[0111] The preparation of embodiment 2 Shengbai oral liquid

[0112] Step (1) Epimedium 200g, psoraleae 100g, aconite (manufactured) 60g, medlar 200g, astragalus 200g, Caulis Spatholobus 200g, madder 200g, angelica 100g, reed root 200g, Ophiopogon japonicus 100g and licorice 100g for later use ;

[0113] Step (2) First put in the prescription amount of Shiwei decoction pieces (excluding Epimedium), add water, heat and decoct until boiling, then add the prescription amount of Epimedium, continue heating and boiling until boiling, extract for 1 hour, filter, Obtain one extraction filtrate, add water to the medicinal dregs and extract once again, filter to obtain the second extraction filtrate, combine the two filtrates, and concentrate the filtrate to extract I with a relative density of 1.24-1.27 (25 ± 5°C);

[0114] Step (3) slowly add 90% ethanol according to extract I: ethanol=1:1~1.2 in extract I, stir while adding, extract extract is dispersed evenly, then add 90% ethanol...

Embodiment 3

[0116] The preparation of embodiment 3 Shengbai oral liquid

[0117] Step (1) Take Epimedium 300g, Psoralen 160g, Aconite (manufactured) 120g, Lycium barbarum 300g, Astragalus 300g, Caulis Spatholobus 300g, Rubia 300g, Angelica 160g, Reed root 300g, Ophiopogon japonicus 160g and Licorice 160g for later use ;

[0118] Step (2) First put in the prescription amount of Shiwei decoction pieces (excluding Epimedium), add water, heat and decoct until boiling, then add the prescription amount of Epimedium, continue heating and boiling until boiling, extract for 1 hour, filter, Obtain one extraction filtrate, add water to the medicinal dregs and extract once again, filter to obtain the second extraction filtrate, combine the two filtrates, and concentrate the filtrate to extract I with a relative density of 1.24-1.27 (25 ± 5°C);

[0119] Step (3) slowly add 90% ethanol according to extract I: ethanol=1:1~1.2 in extract I, stir while adding, extract extract is dispersed evenly, then ad...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Column lengthaaaaaaaaaa
The inside diameter ofaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for establishing a control fingerprint spectrum of Shengbai Koufuye, which comprises the following steps that 1, a test solution is prepared, namely, multiple batchesof qualified Shengbai Koufuye are put into a volumetric flask and the volume is fixed; 2, a reference substance solution is prepared, a reference substance icariin is taken, and the volume is fixed; 3, chromatographic conditions are that octadecylsilane chemically bonded silica is taken as a filler, acetonitrile is taken as a mobile phase A, formic acid water is taken as a mobile phase B, gradientelution of the mobile phase is carried out, the detection wavelength ranges from 200 nm to 300 nm, the column temperature is 25-35 DEG C, the flow velocity is 0.9 ml / min to 1.1 ml / min, and the numberof theoretical plates is not less than 10000 calculated according to icariin peaks; 4, in the determination method, the reference substance solution and the test solution are respectively absorbed, and the solutions are injected into an ultra-high performance liquid chromatograph for determination to obtain a chromatogram; and step 5, common peak confirmation is conducted: the chromatograms of the multiple batches of qualified drugs are processed through a computer model to form a unified chromatogram, and the chromatographic peak with the peak area accounting for more than or equal to 0.8% of the total peak area, good peak pattern and high separation degree is selected as a common peak.

Description

technical field [0001] The invention relates to the field of medicine quality control, in particular to a method for detecting fingerprints of raw white oral liquid. Background technique [0002] Traditional Chinese Medicine Shengbai Oral Liquid is a traditional Chinese medicine preparation for the treatment of leukopenia caused by cancer radiotherapy and chemotherapy. Made from Angelica, Reed Rhizome, Ophiopogon japonicus and Licorice, it has the functions of warming the kidney and strengthening the spleen, and nourishing qi and blood. The drug standardization standard (standard number: WS3-016(Z-002)-2000(Z)) discloses the TLC identification of psoralen, madder, astragalus, and licorice, the limit of aconitine and the high-efficiency solution of icariin Phase chromatographic determination. CN200410078100.2 discloses a quality control method for raw white effervescent granules, including TLC identification of wolfberry, TLC identification of angelica, content determinatio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张敏
Owner HUBEI MONYAN PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap