Identification method of paris polyphylla

A technology of Aescinus aesculus and identification method, which is applied in the field of identification of Aescinus aescini, which can solve the problems of skyrocketing market prices, flooding of commodity markets with rhizomes, and constraints on sustainable development.

Inactive Publication Date: 2017-08-29
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Related companies such as Yunnan Baiyao Group, Sichuan Everbright Pharmaceutical Co., Ltd., and Tonghua Wantong Pharmaceutical Co., Ltd. have a large demand for Rhizoma Rhizome resources, over-exploiting wild resources, and market prices skyrocketing. At present, wild resources of Rhizome Rhizome are becoming endangered. At present, there is no high-quality and stable provenance supply market for artificial planting, which has caused the rhizomes of non-pharmacopoeia plant species to flood the commodity market, which has become a bottleneck restricting the sustainable development of the pharmaceutical industry. Conventional traits, microscopic, physical and chemical, thin-layer identification and other methods for accurate identification, therefore, it is particularly necessary to establish a new identification method for Aescin

Method used

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  • Identification method of paris polyphylla
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  • Identification method of paris polyphylla

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1 infrared fingerprint identification

[0055] Detection method:

[0056] Sample processing: Take the original medicinal material and grind it, pass it through a No. 5 sieve for later use. Take each sample powder and dry at 60°C for 6h. Take 4 mg of each sample separately (to make the light transmittance of the strongest absorption peak below 10), add 200 mg of KBr, mix and grind evenly under an infrared lamp, put it into a mold, press into tablets, and the spectral resolution is 4 cm -1 , measurement area 4000-400cm -1 , medium-speed scanning, scanning times 16 times, instant removal of moisture and CO during scanning 2 The background interference is to ensure the comparability of the maps. In order to reduce the error caused by sample loading, each sample was ground evenly and scanned in parallel 3 times, that is, after each measurement, the sample was poured out and reloaded into a tablet to ensure that the spectrum collection was representative, and t...

Embodiment 2 7

[0070] The composite identification method of embodiment 2 Aescin

[0071] Although no cases of inconsistency with the identification of infrared fingerprints have been found in the current experiments, in order to avoid omissions in the identification of medicinal materials as much as possible and ensure higher accuracy of identification, the present invention also uses a composite identification method, that is, infrared, X-ray diffraction, etc. methods used in combination.

[0072] Wherein, the infrared identification method is as in Example 1.

[0073] The X-ray powder diffraction method is as follows:

[0074] Detection method:

[0075] Sample processing: take a sample of the rhizome of Aesculus chinensis, crush it, dry it at 60°C for 6 hours, and pass it through a 100-mesh sieve for later use. Test conditions: CuKα radiation; scan mode: qualitative, step scan; voltage / current: 36kV / 20mA; scan speed: 8° / min; DS=SS=1°, RS=0.3mm; step width: 0.02°; scan Range 2θ: 5-65°....

Embodiment 3 7

[0088] The composite identification method of embodiment 3 Aescin

[0089] In addition to the method of combining infrared and X-ray powder diffraction identification in Example 2, UPLC can also be added in the present invention.

[0090] Infrared and X-ray powder diffraction identification methods are as in Example 2.

[0091] UPLC identification method is as follows:

[0092] Chromatographic conditions

[0093] ACQUITY HSS C18 column (2.1mm×75mm, 1.7μm); ELSD detector drift tube temperature 55°C, nitrogen pressure 40psi, gain 500, cooling mode; column temperature 40°C; flow rate 0.2mL min -1 ; Injection volume: 2 μL; The mobile phase is acetonitrile (A)-water (B), and the gradient elution program is shown in Table 4. Mixed reference substance chart see Figure 5 .

[0094] Table 4 Gradient elution program

[0095]

[0096] Preparation of the test solution

[0097] Grind the dried rhizome of Aescin, pass through a 40-mesh sieve, accurately weigh 0.500 g of Rhizome...

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Abstract

The invention provides a single and composite identification method of paris polyphylla. The method is realized by using infrared wide spectrum detection. By the method, a genuine product or a counterfeit product of the paris polyphylla can be identified and distinguished through comprising the similarity degree of the standard fingerprint spectrum with the fingerprint spectrum of medicinal materials to be inspected; the novel method is provided for the identification of the paris polyphylla.

Description

technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicinal materials, in particular to an identification method of Aescin. Background technique [0002] Aescin is the dried rhizome of P. polyphylla Smith var. chinensis (Franch.) Hara., the 2015 edition of the "Pharmacopoeia of the People's Republic of China" stipulates that saponins of pachyphylla are used as its quality evaluation standard. Regulated by the Pharmacopoeia as the official source of Chonglou. Steroid saponins are the main active ingredients of Chonglou, which have anti-tumor, anti-inflammatory, hemostatic, antibacterial, analgesic, sedative, anti-oxidant, immune regulation, angiogenesis-inhibiting, liver and kidney protection functions. [0003] Related companies such as Yunnan Baiyao Group, Sichuan Everbright Pharmaceutical Co., Ltd., and Tonghua Wantong Pharmaceutical Co., Ltd. have a large demand for Rhizoma Rhizome resources, over-exploiting wild resour...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/20G01N21/3563
CPCG01N21/35G01N21/3563G01N23/20G01N2021/3595G01N2223/056G01N2223/1016
Inventor 刘圆余孟杰张绍山黄钟杰
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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