Folium artemisiae argyi extract extracted by ethyl acetate and preparation and detection methods and application thereof

A technology of ethyl acetate extraction and ethyl acetate of mugwort leaves, which is applied in the field of detection, ethyl acetate extract of mugwort leaves and its preparation, and can solve problems such as undetermined specific components and the like

Inactive Publication Date: 2014-04-09
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

201210117611.5 is an anti-hepatitis B virus drug, this invention ...

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  • Folium artemisiae argyi extract extracted by ethyl acetate and preparation and detection methods and application thereof
  • Folium artemisiae argyi extract extracted by ethyl acetate and preparation and detection methods and application thereof
  • Folium artemisiae argyi extract extracted by ethyl acetate and preparation and detection methods and application thereof

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Experimental program
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Embodiment 1

[0027] Ethyl acetate extract of Artemisia argyi

[0028] It is prepared by the following method: pulverize mugwort leaves, pass through a 35-mesh sieve to obtain mugwort leaf particles with a particle size of 250 μm, weigh 500 g of mugwort leaf granules, percolate with 2000 ml of petroleum ether, soak in petroleum ether for 18 hours, until the color of the percolation liquid becomes lighter (percolation) The filtrate is about 2500ml), recover the residue, evaporate the petroleum ether in the residue, extract twice with 70% ethanol, each time the amount of ethanol is 2500ml, and each extraction time is 2 hours, the combined extracts are concentrated under reduced pressure to No alcohol smell, relative density is 1.15-1.20, extracted with ethyl acetate, collected the upper layer extract, evaporated to dryness to obtain ethyl acetate extract.

Embodiment 2

[0030] Ethyl acetate extract of Artemisia argyi

[0031] It is prepared by the following method: take mugwort leaves and crush them, pass through a 20-mesh sieve to obtain mugwort leaves particles with a particle size of 850 μm, weigh 500 g of mugwort leaves particles, soak them in 1500 ml of petroleum ether for 24 hours, and obtain residues and leachate (the volume of leachate is about 2000), and The residue was evaporated to dry petroleum ether, extracted twice with 80% ethanol, the amount of ethanol used each time was 1500ml, and the extraction time was 2 hours each time. The two extracts were combined and concentrated under reduced pressure to a relative density of 1.15-1.20 to obtain concentrated ethanol. liquid, extracted with ethyl acetate to obtain the ethyl acetate extract of the upper layer, and dried to obtain the ethyl acetate extract.

Embodiment 3

[0033] Ethyl acetate extract of Artemisia argyi

[0034] It is prepared by the following method: take mugwort leaves and grind them, pass through a 30-mesh sieve to obtain mugwort leaves particles with a particle size of 450 μm, percolate the mugwort leaves with petroleum ether, soak them in 2000ml petroleum ether for 36 hours, and obtain residue and leachate (the volume of leachate is about 2500ml ), evaporate the petroleum ether in the residue, extract with 90% ethanol, extract 3 times, the amount of ethanol is 2000ml each time, the extraction time is 3 hours, combine the extracts, and then concentrate until there is no alcohol smell, and the relative density is 1.15-1.20 , extracted with ethyl acetate, take the ethyl acetate extract of the upper layer, and dry to obtain the ethyl acetate extract, and the obtained Artemisia argyi leaf extract after drying is brown-black.

[0035] The Artemisia argyi leaf extract obtained in the invention has preventive and therapeutic effect...

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Abstract

The invention relates to folium artemisiae argyi extracts, methods and application and particularly relates to a folium artemisiae argyi extract extracted by ethyl acetate and preparation and detection methods and application thereof. Effective ingredients of the folium artemisiae argyi extract consist of flavonoids and sterols. The invention provides application of the folium artemisiae argyi extract in the preparation of anti-tumor drugs, and provides an analysis method for constructing a folium artemisiae argyi extract fingerprint by using an ultrahigh performance liquid chromatography-mass spectrometry (UPLC-MS) analysis technology and analysis results. The method comprises the step of acquiring the UPLC-MS analysis conditions for the folium artemisiae argyi extract, features of chromatographic peaks, compound structure information provided by mass spectrums of chromatographic components, and the like. The fingerprint and technology thereof disclosed by the invention can be applied to the species identification of folium artemisiae argyi and the quality monitoring on the folium artemisiae argyi extracts.

Description

[technical field] [0001] The present invention relates to the extract, method and application of mugwort leaves, in particular to an ethyl acetate extract of mugwort leaves and its preparation, detection method and use. [Background technique] [0002] Artemisia argyi is the dry leaf of Artemisia argyi Lévl.et Vant. It has the effects of warming the meridians to stop bleeding, expelling cold and relieving pain. It is clinically used for vomiting blood, epistaxis, cold pain in the lower abdomen, irregular menstruation, cold uterine infertility, and external use to treat skin itching. It mainly contains volatile oil, flavonoids, triterpenoids, tannins and other ingredients. It has various physiological activities such as hemostasis, antibacterial and antiviral, antitumor, scavenging free radicals, and analgesia. [0003] Domestic Invention 200810052604.5 discloses that Artemisia argyi is extracted with a mixture of ethyl acetate and ethanol as a solvent; the extract is subjec...

Claims

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Application Information

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IPC IPC(8): A61K36/282A61K36/9066G01N30/02A61P35/00
Inventor 陈士林黄林芳任伟光林森森徐宏喜杨大坚卞兆祥陈新滋
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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