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Tibet picrorhiza rhizome composition with specific spectrum effect relationship

A technology of Tibetan rhizoma rhizoma and picloroside, applied in the field of Tibetan rhizoma rhizoma composition with a clear spectrum-effect relationship, can solve problems such as difference in symptom scores

Active Publication Date: 2009-05-13
CHENGDU SINO STRONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after one week of treatment, there was a significant difference in the symptom score (decrease) of the Picroliv group and that of the control group (P<0.05)

Method used

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  • Tibet picrorhiza rhizome composition with specific spectrum effect relationship
  • Tibet picrorhiza rhizome composition with specific spectrum effect relationship
  • Tibet picrorhiza rhizome composition with specific spectrum effect relationship

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Preparation of Huhuanglian Extract

[0047] 1. Take 13kg of Huhuanglian medicinal material, crush it, and set it aside.

[0048] 2. Put the Coptis chinensis coarse powder into a small multi-functional extraction tank, add 8 times the amount (V / W) of 85% ethanol, heat and reflux for extraction for 2 hours, release the extract, and filter. Extract twice more in the same way, combine the extracts into a concentration tank, concentrate in vacuo (temperature: 60°C; vacuum degree: 0.08MPa) to a relative density of 1.10-1.20g / ml, and dry under reduced pressure at 60°C to obtain crude ethanol extract .

[0049] 3. Crush the crude extract, add 6 times the amount (V / W) of acetone, stir and extract at room temperature for 3 times, 20 minutes each time, filter, discard the insoluble matter, combine the acetone extract, and dry under reduced pressure at 60°C to obtain acetone Purified.

[0050] 4. Dissolve the purified acetone with 10 times the amount (V / W) of water, put the solu...

Embodiment 2

[0054] Quality Control of Extract of Coptis chinensis from Tibet

[0055] 【Hairmaking】Refer to the invention section.

[0056] 【Properties】This product is light yellow to light brown powder with extremely bitter taste.

[0057] [Identification] (1) Take 0.1g of the powder of this product, add 5ml of water, shake to dissolve, add 2 drops of 5% ferric chloride ethanol solution, and a dark green precipitate will form.

[0058] (2) Take 0.1g of this product powder, add 5ml of water, shake to dissolve, add 2 drops of 5% α-naphthol ethanol solution, shake well, and form yellow-white turbidity, slowly add 0.5ml of sulfuric acid solution along the tube wall, A purple ring appears at the junction of the two liquids, and the color becomes darker after shaking, and it is diluted with water to form a dark purple precipitate.

[0059] (3) Take this product, add methanol to dissolve and make a solution containing 5mg per 1ml, as the test solution; take another Pipericillin I, II reference...

Embodiment 3

[0071] Anti-hepatic damage effect of extract of Huhuanglian from Tibet

[0072] Table 3. Protective effect of Tibet Huhuanglian extract on liver injury in mice modeled with carbon tetrachloride

[0073]

[0074] Note:" ΔΔ ” means P<0.01 compared with the blank control group.

[0075] " * "" ** ” means P<0.05 compared with the model group; P<0.01.

[0076] From the data analysis in the table, it can be seen that after modeling, except for TB and HBDH, all other indicators in the model group have significant changes, especially the most sensitive index TBA in the current clinical liver function testing items. success. Each Tibet Huhuanglian extract administration group (10-40mg / kg) has different improvement effects on liver function indicators, mainly manifested in the reduction of LDH and HBDH levels in the 10mg / kg group (P<0.05); the change in TB in the 20mg / kg group level (P<0.05); reduce AST, ALT activity (P<0.05).

[0077] The positive drug bifendate mainly change...

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Abstract

The invention relates to a Tibetan picroside extract with a definite spectrum-activity relationship, in particular to a Tibetan picroside extract contained with picroside I (C24H28O11) and picroside II (C23H28O13), and the sum of the contents of the picroside I (C24H28O11) and the picroside II (C23H28O13) is not lower than 50 percent; and on the fingerprint for the extract, the peak area ratio between the picroside II and the picroside I is 1.30 to 2.40 calculated according to the total picroside chromatogram. The extract has the remarkable action of liver injury prevention and has good application prospect in the field of liver injury prevention medication.

Description

technical field [0001] The invention relates to the research and development of a natural medicine extract and the application of the extract in the field of pharmacy. Background technique [0002] Huhuanglian is a perennial low-growing herb of the family Scrophulariaceae, and it is a commonly used medicinal material. It has the effects of clearing heat, dehumidification, improving eyesight, nourishing liver, benefiting gallbladder, and killing insects. Indications for tuberculosis-heat cough, damp-heat diarrhea, malnutrition in children, red eyes and other diseases. In the past, we relied on imports from India, Indonesia, Sikkim, Nepal and other countries to solve domestic medicines. However, due to resources and other reasons, the annual imports are very small, which is far from meeting the needs of disease prevention. Through the census of medicinal materials, it was found that Nyalam, Jilong, Yadong, Cuona, Luozha and other places in Tibet all grow, and the output is r...

Claims

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Application Information

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IPC IPC(8): A61K36/80A61K9/20A61K9/48A61K9/16A61K9/08A61K9/14G01N30/02A61P1/16
Inventor 关文捷谷道昭
Owner CHENGDU SINO STRONG PHARMA
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