Method for extracting suaeda salsa flavone and measuring content of flavones in suaeda salsa
A technology of Suaeda flavonoids and a determination method, which is applied in the field of seaside Chenopodiaceae plant-Suaeda salsa flavonoid extraction and content determination, can solve the problems of impurity interference, high determination content, poor stability of color development and the like, and achieves easy operation, Simple operation and the effect of improving the value of use
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[0023] 1) Extraction of flavonoids: collect seashore plant Suaeda salsa, select stems and leaves, dry and crush; dry at 80°C for 2 hours, add 20-30 times the weight of 70% ethanol to soak and extract for 30 minutes, and process in a microwave oven for 10 minutes. Suction filtration while hot, collect the extract, add petroleum ether with a volume ratio of 1:1 to the ethanol extract, extract 3 to 4 times, remove fat-soluble impurities, transfer the ethanol solution layer to a macroporous adsorption resin column, and mix the stock solution with The ratio of the amount of resin is 1:2, eluted successively with water and 80% ethanol until the solution turns from light yellow to colorless, collects the eluate from the ethanol solution, concentrates the eluate under reduced pressure at 50°C to obtain salt land Suaeda flavonoids extract.
[0024] 2) Prepare the solution to be tested: take the extracted extract of Suaeda salsa, dissolve and dilute it into 100 ml with 70% chromatograph...
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