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Method for extracting suaeda salsa flavone and measuring content of flavones in suaeda salsa

A technology of Suaeda flavonoids and a determination method, which is applied in the field of seaside Chenopodiaceae plant-Suaeda salsa flavonoid extraction and content determination, can solve the problems of impurity interference, high determination content, poor stability of color development and the like, and achieves easy operation, Simple operation and the effect of improving the value of use

Inactive Publication Date: 2013-04-10
YANCHENG INST OF IND TECH
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  • Summary
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  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows creating an extract that contains specific compounds called hesperones or luteolin found naturally within suckled seeds such as Saussurea involucrata. By performing these processes on it's own roots without burning them down like traditional methods, they make more efficient use of resources while producing higher-quality products than existing techniques.

Problems solved by technology

This patented describes methods for extracting specific compounds like flavones from suckerskseas root pods grown throughout Japan. These extracted materials were highly valued because these roots had unique qualifications including being very rare and expensive items. However, current analysis tools require complicated procedures and may result in errors due to factors related to sample preparations. Therefore, new analytical processes aim towards more accurate quantitative measurements while reducing complexity and improving efficiency over existing approaches.

Method used

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  • Method for extracting suaeda salsa flavone and measuring content of flavones in suaeda salsa
  • Method for extracting suaeda salsa flavone and measuring content of flavones in suaeda salsa
  • Method for extracting suaeda salsa flavone and measuring content of flavones in suaeda salsa

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Embodiment Construction

[0023] 1) Extraction of flavonoids: collect seashore plant Suaeda salsa, select stems and leaves, dry and crush; dry at 80°C for 2 hours, add 20-30 times the weight of 70% ethanol to soak and extract for 30 minutes, and process in a microwave oven for 10 minutes. Suction filtration while hot, collect the extract, add petroleum ether with a volume ratio of 1:1 to the ethanol extract, extract 3 to 4 times, remove fat-soluble impurities, transfer the ethanol solution layer to a macroporous adsorption resin column, and mix the stock solution with The ratio of the amount of resin is 1:2, eluted successively with water and 80% ethanol until the solution turns from light yellow to colorless, collects the eluate from the ethanol solution, concentrates the eluate under reduced pressure at 50°C to obtain salt land Suaeda flavonoids extract.

[0024] 2) Prepare the solution to be tested: take the extracted extract of Suaeda salsa, dissolve and dilute it into 100 ml with 70% chromatograph...

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Abstract

The invention relates to a method for extracting suaeda salsa flavone and measuring content of flavone in suaeda salsa. The method comprises the following steps: 1), collecting the suaeda salsa, drying the suaeda salsa and crushing the suaeda salsa; with ethanol as an extractant, soaking the suadeda salsa and carrying out microwave-assisted extracting; filtering; extracting with petroleum ether; transferring the ethanol layer to a macroporous adsorption resin layer; and eluting with water and ethanol aqueous solution sequentially; collecting the eluant, and pressurizing and concentrating the eluant to obtain flavone compound extract; 2), with rutin as a contrast, preparing standard solution with different concentration gradients; measuring the chromatographic peak area at the wavelength of 360nm through a high efficiency liquid chromatography to form flavone content contrast reference; measuring the chromatographic peak area of the to-be-measured solution; obtaining the flavone content of the to-be-measured solution according to the flavone content contrast reference; and obtaining the content of the flavone in the suaeda salsa according to the dilution multiple of the to-be-measured solution. According to the method provided by the invention, soaking and microwave-assisted extracting are adopted to extract flavone, and the content of the flavone is measured by chromatography, so that the method is simple and the solvent is easily available. Besides, the obtained flavone is high in content, and has the physiological and pharmacological activities such as antiviral activity, antioxidant activity, anti-ageing activity and the like.

Description

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Claims

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Application Information

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Owner YANCHENG INST OF IND TECH
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