49 results about "Arg-Gly-Ser" patented technology

The invention discloses polypeptide, a preparation method and an application thereof. An amino acid sequence of the polypeptide is shown as SEQID NO:1. The polypeptide provided by the invention is a bioactive polypeptide extracted from skins of northern leopard frogs, is leucine-valine-arginine-glycine-cysteine-tryptophan-threonine-lysine-serine-tyrosine-proline-proline-lysine-proline-cysteine-phenylalanine-valine-arginine polypeptide, can penetrate in unilamellar lipid vesicles with negative charges, inhibit formation of eosinophilic granulocyte and granulocyte-macrophage colonies and reduce activity of eosinophilic granulocyte for forming precursor cells and mast cells, and achieves the object of treating allergic rhinitis and asthma syndrome by combining active centers of tryptase to inhibit the activity of protease.

The invention relates to a milk caw PAG1 (Pregnancy-associated glycoprotein 1) polypeptide and a polyclonal antibody of the polypeptide. The amino acid sequence of the milk caw PAG1 polypeptide is Asn Asn Ile His Arg Leu Ile Gly Ala Ile Pro Arg Gly Ser Glu His Tyr Val Pro Cys Ser Glu Val Asn Thr. The polypeptide and a carrier protein are crosslinked and are then used for immunizing animals; the immunized animal blood is collected for preparing antiserum; separation and purification are performed to obtain the polyclonal antibody. The antibody is applied to milk caw pregnancy detection, and has the advantages of high specificity, high valence and great application prospects.

The invention belongs to the field of medicine materials, and particularly relates to a coordination copolymer nanometer material as well a preparation method and application thereof. The coordination copolymer nanometer material is characterized in that 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles are taken as a core, silicon dioxide is taken as a shell, and the surface of the shell is modified by a functional material. The preparation method comprises the following steps of: preparing the 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles by a direct precipitation method, then coating the silicon dioxide on the surface of the magnetic nanoparticles so that the surfaces of the 1, 1'-ferrocene dicarboxylic acid-gadolinium nanoparticles carry a quantity of amidogens while the 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles have water solubility, connecting to the surface of the 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles through rhodamine isothiocyanate B and an amidogen effect, and finally connecting arginine glycine aspartic acid tripeptide through the amidogens. The coordination copolymer nanometer material is uniform in particle size and has good dispersity, a very good magnetic resonance imaging effect of T1 and T2, luminescent property and a target function; the synthetic process is simple; raw materials are easily available; and environmental pollution is avoided.

The invention belongs to the field of preparation of composite micelle entrapped drugs, and particularly relates to a composite nano micelle for multi-mode treatment of nasopharyngeal carcinoma and apreparation method and application thereof. The composite nano micelle for multi-mode treatment of nasopharyngeal carcinoma is characterized by comprising Bangladesh rose red and an amphiphilic peptide nano micelle for encapsulating the Bangladesh rose red. According to the invention, the amphiphilic peptide of C18-GRRRRRRRRGDScontaining a tripeptide sequence of arginine, glycine and aspartic is self-assembled into a nano micelle, and the inside of the nano micelle is wrapped with Bangladesh red; the results show that the a combined therapy group has obvious inhibitory effect on CNE-2Z tumor during intravenous injection. During intratumor injection, a photodynamic therapy group (RBNs+Laser) with laser added alone, a sonodynamic therapy group (RBNs+US) with ultrasound added alone and a combination therapy group (RBNs+Laser+US) all have a significant inhibitory effect on tumor growth.

The invention relates to a hirudin polyion micelle composition of a targeted platelet. The compound contains hirudin, arginine-glycine-aspartic acid-polyethylene glycol grafted chitosan and a polyion initiator. The polyion micelle composition can be prepared by a simple method, has platelet targeting, enhances the hirudin anticoagulation and the thrombus proofing activity, effectively suppresses the platelet aggregation and obviously improves the partial thromboplasting time of plasma.

The invention belongs to the field of biomedical materials, in particular to a composite film for guiding tissue repair and a preparation method and application thereof. The composite film for guidingtissue repair comprises a protection layer prepared from chitosan and polyethylene glycol and a tissue repairing layer prepared from hyaluronic acid, sodium alga acid, an arginine-glycine-aspartic acid sequence and nanoparticles, the protection layer achieves a protection effect, and the tissue repairing layer is used for guiding tissue cell growth. The protection layer degradation time is proper, the tissue repairing layer below can be effectively protected, chitosan and chitosan oligosaccharide ingredients are contained, and external bacterium intrusion can be stopped well. The tissue repairing layer of the composite film can continuously release cell factors, and the cells are guided to grow towards the tissue repairing layer; the arginine-glycine-aspartic acid sequence is contained, adhesion of the cell can be improved, and then repairing of injured tissue is promoted.

The invention relates to preparation of polysaccharide-active protein/polypeptide-based active hydrogel microspheres with high cell affinity. Specifically, the hydrogel active microspheres adopt a two-component system, namely (1) water-soluble polysaccharide with a plurality of carboxyl functional groups; and (2) a water-soluble protein or polypeptide chain segment with an arginine-glycine-asparaginic acid sequence (RGD peptide segment) and two or more lysine (amino functional groups) as a cross-linking agent. Through an emulsion method, according to different properties of a gel forming component solution, under the catalytic action of a condensing agent such as 4-(4, 6-dimethoxytriazine-2-yl)-4-methylmorpholine hydrochloride (DMTMM) or 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) and the like, the gel forming component solution is subjected to a condensation reaction in the presence of a catalyst, so that the gel forming component solution is prepared. The two components can be subjected to amidation covalent crosslinking (-(C = O)-N-) in an emulsion system to form hydrogel microspheres. The polysaccharide-active protein/polypeptide-based hydrogel disclosed by the invention has the functions of supporting cell growth, stimulating generation of collagen secreted by fiber cells, promoting generation of normal tissues and the like; the system material is simple in preparation process and excellent in biocompatibility, and can be endowed with multiple functions.

The invention relates to fullerene freeze-dried powder with repairing and anti-aging effects, which is characterized by being prepared from one or more of the following components in percentage by mass: 0.04 to 0.1 percent of fullerene, 3.0 to 5.0 percent of mannitol, 0.1 to 0.2 percent of trehalose, 0.2 to 0.3 percent of glucose, 0.1 to 1.0 percent of histidine, 0.2 to 1.0 percent of arginine, 0.2 to 1.0 percent of glycine, 0.4 to 0.5 percent of polyglutamic acid, 0.1 to 0.2 percent of hyaluronic acid, 1.0 to 2.0 percent of pullulan, 1.0 to 3.5 percent of hydrolyzed collagen and the balance of water. And 90.04 to 91.06% of water. A protective agent and a transdermal absorption promoting agent are added into the freeze-dried powder, so that the fullerene is effectively protected from denaturation and inactivation, and the fullerene can really exert the functions of resisting oxidation, promoting collagen hyperplasia, assisting wound healing and the like.

The invention discloses squalene chidamide prodrug self-assembled nanoparticles, and a preparation method and application thereof; firstly, squalene acid and chidamide are dissolved in methylformamide respectively; N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloric acid are used as catalysts; the two are mixed and react to prepare squalene chidamide prodrug molecules; then, squalene acid and folic acid-polyethylene glycol-amino or p-methoxybenzamide-polyethylene glycol-amino are dissolved in methylformamide respectively; N-hydroxysuccinimide (NHS) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCI) are used as catalysts; the two are mixed and react to obtain a small molecule ligand; and the small molecule ligand, arginine-glycine-aspartic acid and the squalene chidamide prodrug molecules are self-assembled in water to form the nanoparticles. According to the invention, the defects of low permeability, poor targeting property and large toxic and side effects of chidamide in the aspect of solid tumour treatment can be effectively overcome.

The invention discloses an oligopeptide with breast cancer resisting activity. A sequence of the oligopeptide is Phe-Thr-Gln-Tyr-Pro-Lys-Arg-Gly-Ser. The oligopeptide is capable of selectively inhibiting the growth of in vitro breast cancer cells at a lower concentration without influencing the growth of normal breast cells. In vivo experiments indicate that the oligopeptide has a strong function of inhibiting the growth and the proliferation of cancer cells, and shows a remarkable dose-effect relationship. Due to a safe, efficient and perfect cancer resisting effect, the oligopeptide can be used for preparing an anti-cancer medicine, and has a better application prospect.