49 results about "Arg-Gly-Ser" patented technology

All or a portion of a surface of an implantable sensor is covered with a biocompatible coating formed at least partially of a biomaterial matrix having properties that promote a substantially even growth of tissue cells over the surface of the coating. Additional materials, such as growth factors, agents that recruit endogenous stem cells, and cell adhesion motif arginine, glycine, aspartic acid may be included in the coating. Autologous cells may be added to the coating prior to implantation. The sensor surface may also be textured, by etching or abrading, in order to promote even tissue growth. Alternatively, the sensor surface may be covered with a coating having properties that inhibit the growth of tissue. These coatings may include a biomaterial, a biomaterial matrix having a drug, such as a sirolimus or a steroid, an active component, or a self assembled monolayer.

The invention relates to a peptide for high performance inhibition of angiogenesis and method for preparing same and use, wherein high performance blood vessel production inhibiting agent RGD-ED with integration compatibility is designed, the inhibiting agent comprises polypeptide polypeptide-valine-arginine-arginine-alanine-aspartate-arginine-alanine-alanine-valine-praline, its one or two ends are connected with polypeptides containing arginine-glycine-aspartic acid sequence. The RGD-ED provided by the invention can be synthesized. The invention also discloses the expression of one RGD-ED in bacillus coli through gene engineering method, wherein the RGD-ED is prepared through the steps of inclusion body protein segregation, dissolution and renaturation, and ion-exchange chromatography segregation and purification.

The invention relates to a high-performance angiogenesis inhibitor and a production method, which belongs to the field of the biological engineering pharmaceutical technology or protein polypeptide drugs. The invention designs a high-performance angiogenesis inhibitor RGD-ED with integrin compatibility, the inhibitor contains angiogenesis inhibition polypeptide isoleucine-valine-arginine-arginine-alanine-aspartate-arginine-alanine-alanine-valine-proline, and one or two ends of the inhibitor are respectively connected with polypeptides containing arginine-glycin-aspartate sequence. The RGD-ED of the invention can be synthesized. By the method of genetic engineering, the invention also expresses one of RGD-Eds in escherichia coli or other eukaryotic cells, and the RGD-ED is obtained by carrying out the separation, dissolution and renaturation of inclusion body protein and separation and purification by ion exchange chromatography. All the polypeptide sequences of the invention are modified by Polyethylene Glycol (PEG), heparin, dextran, polyvinylpyrrolidone (PVP), polyglycol-poly (amino acid) copolymer, palmitic acid, colominic acid and liposome, which includes liposome (REV), drying liposomal (DRV) and multivesicular liposome (Mvl). In vivo and in vitro experiments, the synthetic polypeptide sequence, product of genetic engineering and modified product of the invention can notably increase the effects of inhibiting the growth of endothelial cells, inhibiting angiogenesis and resisting tumor of the present angiogenesis inhibitors, and moreover, the high-performance angiogenesis inhibitor can be used as a drug curing solid tumors and rheumatoid arthritis.

A major part of medical high polymer materials are hydrophobic and do not have bioactivity, so it is very necessary to perform the biological functionalization modification of the surface of the medical high polymer materials so as to improve the biological compatibility of the materials. By taking poly(hydroxybutyrate-hydroxyvalerate) (PHBV) which is a non-immunogenic biological material for example, the inventor provides the surface biological functionalization method for the hydrophobic medical high polymer materials. Through ammonia plasma treatment or modifying dopamine, amino can be introduced onto the surface of the PHBV, and consequently the surface of the PHBV can be modified with polyethylene glycol molecules (NHS-PEG-MAL) with different functional groups at terminal and short peptides containing arginine-glycine-aspatic acid (RGD). In-vitro cell experiments and protein absorption experiments prove that the biological compatibility of the modified PHBV is improved obviously. The introduced RGD short peptides can promote the growth of the cells on the surface of the material. Meanwhile, the PEG molecules have nonspecific protein absorption resisting capability, and therefore can prevent the generation of inflammation and the formation of thrombi.

The invention discloses polypeptide, a preparation method and an application thereof. An amino acid sequence of the polypeptide is shown as SEQID NO:1. The polypeptide provided by the invention is a bioactive polypeptide extracted from skins of northern leopard frogs, is leucine-valine-arginine-glycine-cysteine-tryptophan-threonine-lysine-serine-tyrosine-proline-proline-lysine-proline-cysteine-phenylalanine-valine-arginine polypeptide, can penetrate in unilamellar lipid vesicles with negative charges, inhibit formation of eosinophilic granulocyte and granulocyte-macrophage colonies and reduce activity of eosinophilic granulocyte for forming precursor cells and mast cells, and achieves the object of treating allergic rhinitis and asthma syndrome by combining active centers of tryptase to inhibit the activity of protease.

The invention relates to a milk caw PAG1 (Pregnancy-associated glycoprotein 1) polypeptide and a polyclonal antibody of the polypeptide. The amino acid sequence of the milk caw PAG1 polypeptide is Asn Asn Ile His Arg Leu Ile Gly Ala Ile Pro Arg Gly Ser Glu His Tyr Val Pro Cys Ser Glu Val Asn Thr. The polypeptide and a carrier protein are crosslinked and are then used for immunizing animals; the immunized animal blood is collected for preparing antiserum; separation and purification are performed to obtain the polyclonal antibody. The antibody is applied to milk caw pregnancy detection, and has the advantages of high specificity, high valence and great application prospects.

The invention belongs to the field of medicine materials, and particularly relates to a coordination copolymer nanometer material as well a preparation method and application thereof. The coordination copolymer nanometer material is characterized in that 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles are taken as a core, silicon dioxide is taken as a shell, and the surface of the shell is modified by a functional material. The preparation method comprises the following steps of: preparing the 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles by a direct precipitation method, then coating the silicon dioxide on the surface of the magnetic nanoparticles so that the surfaces of the 1, 1'-ferrocene dicarboxylic acid-gadolinium nanoparticles carry a quantity of amidogens while the 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles have water solubility, connecting to the surface of the 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles through rhodamine isothiocyanate B and an amidogen effect, and finally connecting arginine glycine aspartic acid tripeptide through the amidogens. The coordination copolymer nanometer material is uniform in particle size and has good dispersity, a very good magnetic resonance imaging effect of T1 and T2, luminescent property and a target function; the synthetic process is simple; raw materials are easily available; and environmental pollution is avoided.

The invention provides an antifreeze polypeptide and preparation method thereof. The method takes cow leather collagen as raw material and obtains the specific antifreeze polypeptide by screening and optimizing the enzymolysis condition of alkali protease, separating and purifying. The antifreeze polypeptide has the molecular weight of 2107Da and the amino acid full sequence of Gly-Glu-Arg-Gly-Phe-Pro-Gly-Glu-Arg-Gly-Ser-Pro-Gly-Ala-Gln-Gly-Leu-Gln-Gly-Pro-Arg. The invention breaks through the research thinking and method of antifreeze protein at home and abroad, overcomes the limitation of the number of purified antifreeze protein in natural organism and the safety worry of international FDA organization for transgene antifreeze protein in the food application, obtains the food-source high-efficiency antifreeze polypeptide, and lays the theoretical foundation for developing the antifreeze polypeptide based on the food source and exploring the wide application of the antifreeze polypeptide in the aspects of food and medical science.

The invention belongs to the field of preparation of composite micelle entrapped drugs, and particularly relates to a composite nano micelle for multi-mode treatment of nasopharyngeal carcinoma and apreparation method and application thereof. The composite nano micelle for multi-mode treatment of nasopharyngeal carcinoma is characterized by comprising Bangladesh rose red and an amphiphilic peptide nano micelle for encapsulating the Bangladesh rose red. According to the invention, the amphiphilic peptide of C18-GRRRRRRRRGDScontaining a tripeptide sequence of arginine, glycine and aspartic is self-assembled into a nano micelle, and the inside of the nano micelle is wrapped with Bangladesh red; the results show that the a combined therapy group has obvious inhibitory effect on CNE-2Z tumor during intravenous injection. During intratumor injection, a photodynamic therapy group (RBNs+Laser) with laser added alone, a sonodynamic therapy group (RBNs+US) with ultrasound added alone and a combination therapy group (RBNs+Laser+US) all have a significant inhibitory effect on tumor growth.

The invention relates to a hirudin polyion micelle composition of a targeted platelet. The compound contains hirudin, arginine-glycine-aspartic acid-polyethylene glycol grafted chitosan and a polyion initiator. The polyion micelle composition can be prepared by a simple method, has platelet targeting, enhances the hirudin anticoagulation and the thrombus proofing activity, effectively suppresses the platelet aggregation and obviously improves the partial thromboplasting time of plasma.

The invention belongs to the field of biomedical materials, in particular to a composite film for guiding tissue repair and a preparation method and application thereof. The composite film for guidingtissue repair comprises a protection layer prepared from chitosan and polyethylene glycol and a tissue repairing layer prepared from hyaluronic acid, sodium alga acid, an arginine-glycine-aspartic acid sequence and nanoparticles, the protection layer achieves a protection effect, and the tissue repairing layer is used for guiding tissue cell growth. The protection layer degradation time is proper, the tissue repairing layer below can be effectively protected, chitosan and chitosan oligosaccharide ingredients are contained, and external bacterium intrusion can be stopped well. The tissue repairing layer of the composite film can continuously release cell factors, and the cells are guided to grow towards the tissue repairing layer; the arginine-glycine-aspartic acid sequence is contained, adhesion of the cell can be improved, and then repairing of injured tissue is promoted.

A method of treating seizures, epilepsy or loss of brain function in an individual comprising the steps of preparing a composition composed of an all-D amino acid peptide and a pharmaceutically acceptable carrier.The D peptide has the general structure: A-B-C-D-E in whichA is Ser, Thr, Asn, Glu, Ile.B is Ser, Thr, Asp, Asn,C is Thr, Ser, Asn, Arg, Lys, Trp,D is Tyr, andE is Thr, Ser, Arg, Gly.And wherein all amino acids in the D peptide are the D stereoisomeric configuration and said peptide composition is administered in a therapeutically effective dose wherein said composition acts to suppress inflammation underlying the loss of brain function. The D peptide may be esterified, glycosylated, or amidated at E to enhance tissue distribution by promoting egress from the circulation and penetration into the brain.

The invention relates to an injectable active bone-repair material and a preparation method thereof. The injectable active bone-repair material is prepared from calcium sulfate hemihydrate and arginine, glycine and aspartic acid (RGD)-modified porous hydroxyapatite by adopting a high-energy ball milling dispersing technology. The injectable active bone-repair material provided by the invention has the characteristics of being good for the ingrowth of cells and the formation of new bones and being beneficial to promoting the anchorage-dependent growth of osteoblasts in a human body and accelerating the formation of new bones. In addition, the injectable active bone-repair material has the advantages of being injectable and incapable of causing immunological rejection reaction and can meet the needs of clinical treatment of bone defects.

The invention relates to a peptide for high performance inhibition of angiogenesis and method for preparing same and use, wherein high performance blood vessel production inhibiting agent RGD-ED with integration compatibility is designed, the inhibiting agent comprises polypeptide polypeptide-valine-arginine-arginine-alanine-aspartate-arginine-alanine-alanine-valine-praline, its one or two ends are connected with polypeptides containing arginine-glycine-aspartic acid sequence. The RGD-ED provided by the invention can be synthesized. The invention also discloses the expression of one RGD-ED in bacillus coli through gene engineering method, wherein the RGD-ED is prepared through the steps of inclusion body protein segregation, dissolution and renaturation, and ion-exchange chromatography segregation and purification.

The invention provides in-situ injectable hydrogel for treating central nervous injury and a preparation method of the in-situ injectable hydrogel. The hydrogel is formed by performing click chemical coupling on X-Y through functional groups on arginine-glycine-aspartic acid modified multi-arm polyethylene glycol-X and multi-arm polyethylene glycol-Y, nano / micron particles and growth factors of slow-release immunoregulation and / or antioxidant drugs loaded on the hydrogel through chemical coupling reaction are coated in the hydrogel. The injection hydrogel disclosed by the invention can be used for precisely and slowly releasing the immunomodulatory and / or antioxidant drug and the growth factor locally at an injured part, so that the formation of a cystic cavity after spinal cord injury is prevented, secondary injury caused by neuroinflammation is relieved, residual spinal cord nervous tissues and axons are protected, and the formation of glial scar tissues is reduced; a penetrable extracellular matrix environment is provided for regeneration of body nerve axons, further recovery of electrophysiology and motor functions is promoted, and the method can be used for repairing injury of soft tissues such as spinal cords and the like.

The invention provides an antifreeze polypeptide and preparation method thereof. The method takes cow leather collagen as raw material and obtains the specific antifreeze polypeptide by screening and optimizing the enzymolysis condition of alkali protease, separating and purifying. The antifreeze polypeptide has the molecular weight of 2107Da and the amino acid full sequence of Gly-Glu-Arg-Gly-Phe-Pro-Gly-Glu-Arg-Gly-Ser-Pro-Gly-Ala-Gln-Gly-Leu-Gln-Gly-Pro-Arg. The invention breaks through the research thinking and method of antifreeze protein at home and abroad, overcomes the limitation of the number of purified antifreeze protein in natural organism and the safety worry of international FDA organization for transgene antifreeze protein in the food application, obtains the food-source high-efficiency antifreeze polypeptide, and lays the theoretical foundation for developing the antifreeze polypeptide based on the food source and exploring the wide application of the antifreeze polypeptide in the aspects of food and medical science.

A thrombus target fusion mA5UKB with both anticoagulating and thrombolytic functions is prepared from the mutant mAnxA5 of AnnexinA5 having anti-coagulating function and the urokinase B chain (UKB) having thrombolytic function through fusing. It can be used to prepare the thrombolytic medicine.

The invention relates to the technical field of biology and particularly discloses a method for artificial synthesis of antimicrobial peptides. The method comprises step S1 of taking an appropriate amount of amino acid raw materials, adding vegetable oil and phosphoric acid, baking the mixture for 2 h at 150 DEG C, wherein the volume ratio of vegetable oil to phosphoric acid is (17-30):1; and step S2 of taking out a polymer and using absolute ethyl alcohol to perform washing, centrifugation and drying to obtain the product antimicrobial peptides. The amino acid raw materials contain lysine, leucine and cysteine and one or more of arginine, glycine and serine; and the ratio of cysteine to lysine to leucine is 9:(7-9):(4.5-14) and is a weight ratio. The method for artificial synthesis of the antimicrobial peptides is simple in production process, short in synthesis time and low in cost and can be used for large-scale production, and the antimicrobial peptides obtained through synthesis have broad-spectrum antimicrobial activity.

The invention relates to preparation of polysaccharide-active protein / polypeptide-based active hydrogel microspheres with high cell affinity. Specifically, the hydrogel active microspheres adopt a two-component system, namely (1) water-soluble polysaccharide with a plurality of carboxyl functional groups; and (2) a water-soluble protein or polypeptide chain segment with an arginine-glycine-asparaginic acid sequence (RGD peptide segment) and two or more lysine (amino functional groups) as a cross-linking agent. Through an emulsion method, according to different properties of a gel forming component solution, under the catalytic action of a condensing agent such as 4-(4, 6-dimethoxytriazine-2-yl)-4-methylmorpholine hydrochloride (DMTMM) or 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride / N-hydroxysuccinimide (EDC / NHS) and the like, the gel forming component solution is subjected to a condensation reaction in the presence of a catalyst, so that the gel forming component solution is prepared. The two components can be subjected to amidation covalent crosslinking (-(C = O)-N-) in an emulsion system to form hydrogel microspheres. The polysaccharide-active protein / polypeptide-based hydrogel disclosed by the invention has the functions of supporting cell growth, stimulating generation of collagen secreted by fiber cells, promoting generation of normal tissues and the like; the system material is simple in preparation process and excellent in biocompatibility, and can be endowed with multiple functions.

A formulation and method for treating or reducing ocular surface inflammation and associated diseases and disorders. The formulation includes targeted micelles that encapsulate tacrolimus within a pharmaceutically acceptable carrier, wherein the formulation is coated in arginine-glycine-aspartic acid peptide.

The invention relates to fullerene freeze-dried powder with repairing and anti-aging effects, which is characterized by being prepared from one or more of the following components in percentage by mass: 0.04 to 0.1 percent of fullerene, 3.0 to 5.0 percent of mannitol, 0.1 to 0.2 percent of trehalose, 0.2 to 0.3 percent of glucose, 0.1 to 1.0 percent of histidine, 0.2 to 1.0 percent of arginine, 0.2 to 1.0 percent of glycine, 0.4 to 0.5 percent of polyglutamic acid, 0.1 to 0.2 percent of hyaluronic acid, 1.0 to 2.0 percent of pullulan, 1.0 to 3.5 percent of hydrolyzed collagen and the balance of water. And 90.04 to 91.06% of water. A protective agent and a transdermal absorption promoting agent are added into the freeze-dried powder, so that the fullerene is effectively protected from denaturation and inactivation, and the fullerene can really exert the functions of resisting oxidation, promoting collagen hyperplasia, assisting wound healing and the like.

The present invention discloses one new type of double function RGD glucokinase molecule and its preparation process and use. The double function RGD glucokinase molecule is glucokinase or its derivative with the amino acids in places 35, 36 and 37 substituted successively into arginine R, glycine G and aspartic acid D. The preparation process of the mutant includes the steps of: site-directed mutation of glucokinase gene, connection to the expression vector, high efficiency expression in colibacillus, separation and purification, etc. The glucokinase mutant of the present invention may be used as thrombolytic medicine and has both thrombolytic and anticoagulant activity.

The invention discloses a thrombus targeting long circulating polycation micelle, and a preparation method and applications thereof. Through an EDC / NSH esterification system, poly(2-ethyl-2-oxazoline) is used to graft chitosan so as to prepare the polyion micelle; modification is performed on chain ends by using arginine-glycine-aspartic acid sequence short peptide; and through the carrying of anticoagulant and antithrombotic drugs, the thrombus targeting long circulating polycation micelle can be obtained. Through the prepared thrombus targeting polyion micelle, the purposes of improving protein drug stability, targeting thrombus, prolonging the action time in vivo and reducing immunogenicity can be achieved. The preparation method is feasible and reliable in operation; the obtained carrier is high in drug loading; the drugs have obvious thrombus targeting performance and are long in action time in vivo; and therefore, theoretical basis can be provided for the research of a novel thrombus targeting delivery system.

The present invention discloses a novel composition of the elution mobile phase for virus purification by immunoaffinity chromatography which is consisting of one or more amino acids: L-serine, L-asparagine, or L-glutamine, or their salts with pharmaceutically acceptable acids; one or more auxiliary ingredients: L-arginine, glycine or imidazole, or their salts with pharmaceutiacally acceptable acids; one or more pharmaceutically acceptable pH adjusting agents for correcting the pH value of the mobile phase from pH=6.0-8.0; and purified water, up to 100% w / w of the mobile phase composition. The invention provides the use of immunoaffinity chromatography as a key step in the production of viral vaccines and / or viral vectors, in separation of infectious from non-infectious viral particles, and for enrichment of the viral suspension in infectios viral particles.

The invention discloses drinking liquid for increasing estrus rate of weaning sows and application thereof. Each L of the drinking liquid contains 20-40 g of lysine, 10-20 g of methionine, 4-10 g of tryptophan, 10-20 g of threonine, 10-20 g of valine, 10-15 g of histidine, 10-20 g of arginine, 20-40 g of glycine, 5-10 g of histidine, 20-40 g of glutamic acid, 5-15 g of vitamin B1, 5-15 g of vitamin B2, and 5-10 g of vitamin B12. All the amino acids and vitamins are of food grade, and solution is obtained by preparing in a scientific way. The drinking liquid is high in safety; post-weaning estrus rate of the weaning sows feeding on 30-50 ML of the liquid each day can be increased significantly, false pregnancy period of the sows is decreased, and conception rate is increased.

The invention belongs to the field of medicine materials, and particularly relates to a coordination copolymer nanometer material as well a preparation method and application thereof. The coordination copolymer nanometer material is characterized in that 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles are taken as a core, silicon dioxide is taken as a shell, and the surface of the shell is modified by a functional material. The preparation method comprises the following steps of: preparing the 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles by a direct precipitation method, then coating the silicon dioxide on the surface of the magnetic nanoparticles so that the surfaces of the 1, 1'-ferrocene dicarboxylic acid-gadolinium nanoparticles carry a quantity of amidogens while the 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles have water solubility, connecting to the surface of the 1,1'-ferrocene dicarboxylic acid-gadolinium nanoparticles through rhodamine isothiocyanate B and an amidogen effect, and finally connecting arginine glycine aspartic acid tripeptide through the amidogens. The coordination copolymer nanometer material is uniform in particle size and has good dispersity, a very good magnetic resonance imaging effect of T1 and T2, luminescent property and a target function; the synthetic process is simple; raw materials are easily available; and environmental pollution is avoided.

The invention discloses a tumor targeted cell-penetrating cyclodextrin derivative as well as a preparation method and application thereof. The cyclodextrin derivative comprises a targeting cell-penetrating peptide R6RGD and a carrier material carboxymethyl-beta-cyclodextrin, and the carboxymethyl-beta-cyclodextrin is beta-cyclodextrin which is modified by cyclodextrin, has strong hydrophilicity and contains a carboxyl terminal. The targeting cell-penetrating peptide R6RGD is a polypeptide fragment which has a cell-penetrating capability and is specifically targeted to tumor cells and is formedby combining a hexaarginine (R6) cell-penetrating peptide having a cell-penetrating capability with a tripeptide of RGD (arginine-glycine-aspartic acid) with a tumor cell targeting function. The targeting cell-penetrating peptide R6RGD is bonded with carboxymethyl-beta-cyclodextrin amido bonds with outer hydrophilicity and inner hydrophobicity to form a stable nano inclusion material. The sustained release preparation of the tumor targeted cell-penetrating cyclodextrin derivative has good biocompatibility and stability, has the advantages of low in-vivo toxic and side effects, strong membranepenetrating capability, good tumor targeting and the like, and is simple in preparation method, convenient to operate and high in yield.

The invention discloses squalene chidamide prodrug self-assembled nanoparticles, and a preparation method and application thereof; firstly, squalene acid and chidamide are dissolved in methylformamide respectively; N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloric acid are used as catalysts; the two are mixed and react to prepare squalene chidamide prodrug molecules; then, squalene acid and folic acid-polyethylene glycol-amino or p-methoxybenzamide-polyethylene glycol-amino are dissolved in methylformamide respectively; N-hydroxysuccinimide (NHS) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCI) are used as catalysts; the two are mixed and react to obtain a small molecule ligand; and the small molecule ligand, arginine-glycine-aspartic acid and the squalene chidamide prodrug molecules are self-assembled in water to form the nanoparticles. According to the invention, the defects of low permeability, poor targeting property and large toxic and side effects of chidamide in the aspect of solid tumour treatment can be effectively overcome.