101 results about "17 beta estradiol" patented technology

A composition of matter is provided having a mixture of active estrogenic compounds. The mixture is present in chemically pure form. The mixture includes salts of conjugated estrone, conjugated equilin, conjugated Δ8,9-dehydroestrone, conjugated 17α-estradiol, conjugated 17α-dihydroequilin, conjugated 17β-dihydroequilin, conjugated 17β-estradiol, conjugated equilenin, conjugated 17α-dihydroequilenin, and conjugated 17β-dihydroequilenin. The mixture also contains the same essential estrogenic compounds present in naturally derived equine conjugated estrogens. Drug products including the composition of matter are also provided, as are methods of using these drug products to treat mammals in need of treatment. Methods of analyzing mixtures containing conjugated estrogens are also provided.

The formulation comprises a combination of three estrogens and selected amount of other elements. The three estrogens include 2-hydroxyestrone, 17-beta estradiol, and estriol. The amount of 17-beta estradiol is substantially less than the amounts of 2-hydroxyestrone and estriol, both which are approximately equal in amount. The amounts of pyridoxine, folic acid, selenium and cobalt are therapeutically effective amounts.

A pharmaceutical dosage unit for oral administration to a human female comprising a therapeutically effective amount of 17β-estradiol-3-lower alkanoate, most preferably 17β-estradiol-3-acetate, and a pharmaceutically acceptable carrier is disclosed. Also disclosed is a method for treating a human female in need of 17β-estradiol and a contraceptive method by oral administration of the pharmaceutical dosage unit and a method of preparing a pharmaceutical composition that may be used to form the pharmaceutical dosage unit of the invention.

The invention relates to a detection technology of endocrine disrupters in water environment, in particular relating to a method for detecting seven substances such as oestrone, 17 beta-estradiol, estriol, 17 alpha-ethinyl estradiol, nonyl phenol, octylphenol and bisphenol A and the like in a water environment sample by virtue of liquid chromatogram-tandem mass spectrometry. The method comprises the following steps: concentrating and gathering the estrogen, the nonyl phenol, the octylphenol and the bisphenol A in a water sample by utilizing a solid phase extraction column; eluting the estrogen, the nonyl phenol, the octylphenol and the bisphenol A from the solid phase extraction column by utilizing ethyl acetate; and analyzing the concentrations of the estrogen, the nonyl phenol, the octylphenol and the bisphenol A by virtue of liquid chromatogram-tandem mass spectrometry. The method has the advantages of environment friendliness and easiness for operation, ensures relatively high recovery, and can be used for fast analyzing the concentrations of the estrogen, nonyl phenol, octylphenol and bisphenol A in the water sample.

The invention relates to the technology for detecting trace estrogens in sludge, in particular to a method for detecting trace oestrone and 17beta-estradiol content in a sludge sample. The method comprises the following steps of: performing freeze drying on the collected sludge sample, adding NaN3, performing oscillation extraction by adopting acetone, performing quick solvent extraction, and merging the extract liquor; and concentrating and enriching estrogens in the extract liquor by adopting a solid phase extraction column, washing the estrogens off from the solid phase extraction column by adopting ethyl acetate, and analyzing the estrogen concentration by utilizing liquid chromatography tandem mass spectrometry. The method has the advantages of environmental protection, easy operation and high recovery rate, and can quickly analyze the trace oestrone and 17beta-estradiol content in the sludge sample.

The invention relates to a detecting technique of endocrine disrupters in water environment and particularly relates to a technique for quantitative analysis on oestrone, 17beta-estradiol, estriol, 17alpha-ethinyl estradiol, nonyl phenol, octylphenol and bisphenol A in a complex substrate water sample by adopting a liquid chromatogram-tandem mass spectrum combined technique. The method comprises the following steps: enriching a collected water sample by using a HLB (Hydrophile-Lipophile Balance) solid-phase extraction column and washing with carbinol; drying elution liquid nitrogen and purifying by using Florisil; and finally, analyzing by utilizing the liquid chromatogram-tandem mass spectrum combined technique. The method is environment-friendly, easy to operate and high in recovery rate. The method can quickly analyze the trace amount of oestrone, 17beta-estradiol, estriol, 17alpha-ethinyl estradiol, nonyl phenol, octylphenol and bisphenol A in the complex substrate water sample.

The present invention discloses a 17beta-estradiol visualization detection method based on DNA nano-structure, and a 17beta-estradiol visualization detection kit based on DNA nano-structure. The working principle is that 17b-estradiol interacts with aptamer, a strand displacement reaction is started, three groups of stem-loop DNA probes are constantly opened to form DNA nano-structures, a G tetramer having catalysis activity is formed on the terminals of the three DNA arms in the DNA nano-structures, the G tetramer is bound with hemin so as to form a compound having catalysis activity and similar to HRP, TMB is subjected to catalytic oxidation, and a blue substrate is produced, wherein the result is visible, and the concentration of 17b-estradiol is directly related to the blue shade. According to the present invention, the operation is simple, the whole reaction can be completed at the room temperature, the high sensitivity is provided, the detection limit on 17b-estradiol is 100 fM, the good specificity is provided, the detection result is directly visible without any detection equipment, and the method and the kit can be used for the on-site rapid detection of 17b-estradiol.

The invention relates to a method for preparing a photoelectric adapter sensor for detecting 17beta-estradiol. The photoelectric adapter sensor takes a prepared cadmium selenide nano particle modified titanium dioxide nano tube as a matrix electrode; after the surface of the electrode is functionalized by chitosan and glutaraldehyde, and a 17beta-estradiol adapter is dropwise added onto the surface of the electrode so as to prepare the 17beta-estradiol photoelectric adapter sensor. Compared with the prior art, the method combines the 17beta-estradiol photoelectric adapter sensor with specific recognition capability with a supersensitive photoelectric analysis technique for detecting an environmental internal-secretion interfering-substance 17beta-estradiol, so that not only is high sensitivity acquired, but also interference of a complex matrix environment is prevented, and high selectivity is achieved; when the method is used in low-concentration 17beta-estradiol detection, a wide working curve range of 5*10<-13> mol / L to 15*10<-12> mol / L is obtained, the minimum detection limit can be 3.3*10<-14> mol / L, and moreover, the 17beta-estradiol photoelectric adapter sensor is simple in preparation method, low in cost and good in stability and repeatability.

A hormone replacement therapy, comprising a plurality of daily doses of a pharmaceutical preparation, the doses being administered continuously and consecutively in alternating phases of three daily doses, a relatively dominant estrogenic activity phase comprising three daily doses of a substance exhibiting estrogenic activity equivalent to about 1 mg per day of 17beta-estradiol per day, and a relatively dominant progestagenic activity phase of a combination of a substance exhibiting estrogenic activity equivalent to about 1 mg per day of 17beta-estradiol and a substance exhibiting progestogenic activity equivalent to about 90 mug per day of norgestimate.

The invention discloses a preparation method for a 17 beta-estradiol molecular imprinting film electrochemical sensor. In the method, the 17 beta-estradiol molecular imprinting film electrochemical sensor is prepared through the steps in sequence as follows: pretreating an electrode, modifying the electrode by nanometer platinum particles, modifying the electrodes by a molecular self-assembly film, carrying out an electropolymerization reaction and eluting template molecules. The molecular imprinting electrochemical sensor prepared by the invention has high selectivity, rapid response, high sensitivity, high stability and high tolerance, is capable of realizing high-efficiency, sensitive and real-time detection on trace 17 beta-estradiol in a water sample and has an important significance in monitoring environmental pollutants.

The invention discloses a derivatization method of environmental estrogens (estrone, 17beta-estradiol, estriol, 17alpha-ethynylestradiol), which is a treatment method and a core technology before a gas chromatography-mass spectrum (GC-MS) is used for analyzing the samples of the substances. The method comprises the following steps: firstly, leading high-purity nitrogen in at a room temperature to dry a standard mixed solution containing environmental estrogens; adding derivative reagents N, O-BSTFA and pyridine to react at room temperature (20 DEG C)-50 DEG C to obtain derivative products; drying; adding a sampling solvent and an internal standard; sampling and analyzing. The method has the advantages of simple and convenient experimental operation, time saving and low energy consumption and cost, simultaneously derivates the four environmental estrogens without generating side products, has favorable linear correlation among various derivative products and achieves an instrument detection limit up to 0.5pg / muL, thereby being applied to the GC-MS detection of environmental estrogens in water bodies, sediment, biological samples, and the like.

The invention provides an electrochemical immunosensor for detecting 17 beta-estradiol. According to the immunosensor, an electrode surface is modified by a prepared graphene oxide-polyaniline (GO-PANI) compound through an electrode surface modification technology, a platinum-gold alloy (Au-PtNPs) layer is polymerized on the electrode surface with an electro-polymerization method, 17 beta-estradiol complete antigen is modified on the electrode surface, and an antibody compound (PDA-PtNPs-AuNPs-Ab-HRP) and a 17 beta-estradiol standard substance are simultaneously modified on the electrode surface, so that the complete antigen and the standard substance simultaneously compete and combine with an antibody on an antibody compound, a signal is generated by an HRP catalytic substrate H2O2 combined on the antibody compound on the electrode surface, a working curve is drawn according to the relation between signal changes before and after adding of the substrate H2O2 and standard substance concentration, and the 17 beta-estradiol is detected. A PDA-PtNPs-AuNPs nano material is synthesized for the first time, the conductivity of the GO-PANI compound is high, and the Au-PtNPs nano alloy has excellent biocompatibility and certain catalytic performance on H2O2; and the electrochemical immunosensor has the advantages of high sensitivity, high detection speed and short detection period, and an actual sample can be detected rapidly and conveniently.

A monophasic method of achieving contraception in a human female comprising orally administering to the human female a composition comprising 1.5 mg of 17-beta-estradiol and 2.5 mg of nomegestrol acetate for 24 days followed by a hormone-free period of 4 days.

The present invention relates to the preparation method and the application of a 17 beta-estradiol molecularly-imprinted polymer, which belongs to the analysis and measurement technology field of the 17 beta-estradiol of the biological samples and food samples. The present invention proposes a method for the preparation of 17 beta-estradiol molecularly-imprinted polymer as well as the separation, purifying and examination method of the 17 beta-estradiol molecularly-imprinted polymer in the biological samples and food samples. The 17 beta-estradiol molecularly-imprinted polymer generated by the present invention is of selective absorption. The present invention can separate, purify, concentrate and fast examine and analyze the 17 beta-estradiol molecularly-imprinted polymers in the environmental, biological samples and food samples.

The invention belongs to the field of materials science and engineering and environmental sciences, and particularly relates to a method for preparing an estradiol molecularly imprinted magnetic microsphere in a controllable living polymerization potful compositing mode. According to the method, a reversible addition-fracture chain transfer controllable living free radical polymerization and precipitation polymerization potful compositing mode is adopted, molecularly imprint with 17 beta estradiol as a template and a sensitive material are combined, and then the estradiol molecularly imprinted magnetic microsphere is obtained. The microsphere obtained through the method is high in adsorption capacity for 17 beta estradiol, has the high-speed dynamic character and the superior recognizing and selecting performance, is separated conveniently and quickly under the action of an applied magnetic field, and is good in reusing performance. According to the method, the character of reversible addition-fracture chain transfer controllable living polymerization is utilized fully, step-by-step feeding potful operation is adopted to prepare the core-shell structure printed microsphere, the surface printing process is effectively simplified, the experimental period is shortened, the recognizing speed is greatly improved, and the printing capacity is increased.

The invention relates to an electrochemical analyzing method for constructing a 17beta-estradiol aptamer sensor based on a dendritic gold modification boron-mixed diamond film BDD electrode and provides an electrochemical analyzing method high in sensitivity and high in selectiveness. The method comprises the steps of manufacturing special-morphology nanometer dendritic gold / BBD electrodes based on an electrochemistry method and further performing nucleic acid aptamer load assembly by adopting the surface characteristics of the modified electrode to construct the 17beta-estradiol aptamer sensor, wherein the sensor is used for detecting environmental internal-secretion interfering-substance, namely, 17beta-estradiol. Compared with the prior art, a dendritic gold structure with multiple levels of active sites is constructed on the BDD substrate electrode with a simple and novel double-template method, a nucleic acid aptamer capable of being combined with target substances in a peculiar mode is adopted at the same time, noise in the analyzing process is effectively lowered, the detection sensitivity and selectiveness of the sensor are greatly improved, the detection limit reaches 5*10<-15> mol / L, and the sensor has good reproducibility.

The invention belongs to the technical field of assisted reproduction, and particularly relates to a oocyte in-vitro maturity nutrient solution and a preparation method thereof. The oocyte in vitro manturation culture medium is based on commercialized tissue nutrient solution TCM199, and accurately added commercialized constituents comprise 0.075-0.10 IU / ml FSH and 0.5-0.8 IU / ml HCG, 0.1-0.2 microgram / ml 17 beta-estradiol, and human commercialized replaced serum or human albumin with the volume percent being of 10-20 percent. The oocyte in-vitro maturity nutrient solution is stable in constituents and quality, can be industrially produced. By adding commercialized human replaced serum and serum albumin, improving the concentration of the estradiol, FSH and HCG in the culture medium and simulating in-vivo mature environment in human oocyte, a good clinical effect is obtained, the in vitro maturity rate of oocyte reaches 72.08 percent, and the clinical pregnancy rate reaches 23.71 percent.

Pseudomonas fluorescens capable of degrading estrogen substances is named as SJTE-2, and a preservation number is CGMCC No.6587. The pseudomonas fluorescens can use natural estrogen or environmental estrogen as the only carbon source to grow and can degrade estrogen substance oestrone, 17-Beta-estradiol, estriol and polycyclic aromatic hydrocarbon substance naphthalene, luxuriant, bisphenol A, and can be used for decomposing and removing an estrogen substance and a polycyclic aromatic hydrocarbon substance in an environment. Under the culture condition that the estrogen or the polycyclic aromatic hydrocarbon is used as the only carbon source, a bacterial strain SJTE-2 can degrade oestrone, 17-beta-estradiol and estriol within 24 hours, wherein the initial concentration of the oestrone, 17-beta-estradiol and estriol is 1 mg / L, and degradation rate of naphthalene, luxuriant and bisphenol A is over 90%, wherein the concentration of the naphthalene, luxuriant and bisphenol A is 50 mg / L. The bacterial strain can degrade oestrone, 17-beta-estradiol and estriol for more than 90% in 7 days, wherein the initial concentration of oestrone, 17-beta-estradiol and estriol is 50 mg / L, and degradation rate of naphthalene, luxuriant and bisphenol A is larger than 80%, wherein the concentration of naphthalene, luxuriant and bisphenol A is 500 mg / L.

The invention relates to a detecting technique for an endocrine disrupter in a water environment and especially relates to a co-detection method for an estrogen coalition, Estrone-3-sulfate, E1-3S, Estradiol-3-sulfate, E2-3S, Estradiol-3-glucuronide, E1-3G and17beta-Estradiol-3-glucuronide, E2-3G in a water sample by adopting a liquid chromatogram-tandem mass spectrum technique. The method comprises the steps of: enriching the E1-3S, E2-3S, E1-3G and E2-3G in a collected water sample by using a HLB (Hydrophile-Lipophile Balance) solid-phase extraction column; connecting an activated NH2 column under the HLB column and washing with carbinol; and lastly, eluting with 2% of ammonia water carbinol solution and analyzing by adopting the liquid chromatogram-tandem mass spectrum technique. The method has the advantages of environmental protection, easiness in operation, high recovery rate and quick analysis for the trace amount of estrogen coalition E1-3S, E2-3S, E1-3G and E2-3G in the watersample.

The invention discloses a nutrient solution for maturation of oocyte in vitro and a method for culturing oocyte using the nutrient solution, and relates to a nutrient solution for nutrient solution for oocyte and a method for culturing the oocyte with the nutrient solution. The nutrient solution and the culturing method using the nutrient solution solve the problem that the viability of oocyte culturing in vitro is low. The nutrient solution for maturation of oocyte in vitro is composed of normal nutrient solution of oocyte, vitamin B12, fetal calf serum, FSH, LH, 17 betas estradiol, sodium pyruvate, EGF, penicillin and streptomycin sulphate. The oocyte is placed into the utrient solution for maturation of oocyte in vitro, the maturation culture on the oocyte lasts for 20-24 hours in the environment in which the volume concentration of CO2 is 5%, and a mature oocyte is acquired. The nutrient solution for maturation of oocyte in vitro can decrease stress injury caused by the external environment, the cytoplasm maturing of the oocyte is promoted, and the parthenogenetic activation of the matured oocyte matured in vitro and the embryos developmental potential after being fertilized are improved.

The invention discloses a method for detecting 17beta-estradiol by employing a colorimetric method based on a nucleic acid aptamer. The method comprises the following steps: drawing a standard curve, namely preparing a 17beta-estradiol standard system, recording absorbance values of a standard solution and a contrast solution under the conditions of wavelengths of 650nm and 520nm, plotting by virtue of the specific value difference Delta A between the relative absorbance corresponding to the standard solution and the contrast solution, and drawing a standard curve; detecting a 17beta-estradiol sample, namely adding a to-be-detected sample to a buffer solution containing the nucleic acid aptamer, mixing evenly, incubating and simultaneously adding water to obtain the contrast solution; adding a cationic polymer solution, mixing evenly and incubating; adding a nano gold solution, mixing evenly and incubating; and calculating the 17beta-estradiol content in the to-be-tested sample, namely determining the concentration of the 17beta-estradiol in the to-be-tested sample according to the standard curve corresponding to the difference value Delta A between A650 / A520 measured by the sample and the A650 / A520 of a blank control group.

The invention provides a 17beta-estradiol molecular imprinted silver-doped TiO2 nanotube and a preparation method thereof. The material can selectively adsorb 17beta-estradiol and degrade 17beta-estradiol and is doped with silver to powerfully kill certain pathogenic microorganisms. The TiO2 nanotube comprises a TiO2 nanotube with two open ends, a molecular imprinting polymer and Ag simple substance, which are formulated in a mass ratio of 58:33:9, wherein the molecular imprinting polymer contains methacrylic acid, trimethylolpropane trimethylacrylate and azo-biscyanopentanoic acid, which are formulated in a mole ratio of 124:123:1, and the molecular imprinting polymer is loaded with carbonyl functional groups; the TiO2 nanotube is 90 nm in diameter, 600 nm in length, 0.51 cm3 / g in porosity and 290 m2 / g in specific surface area ; and the mole ratio of functional monomer methacrylic acid to template molecule 17beta-estradiol is 124:26. The 17beta-estradiol molecular imprinted silver-doped TiO2 nanotube has a good 17beta-estradiol adsorbing effect, can degrade 17beta-estradiol with high degradation rate up to 98%, can powerfully kill certain pathogenic microorganisms, and is significant in improving the quality of reclaimed water from sewage.

The invention relates to a pseudomonas citronelloalis strain and application thereof in degrading estrogen, wherein the pseudomonas citronelloalis strain has the preservation number of CGMCC No.3634, can grow by using natural or synthetic estrogen as a unique carbon source, can efficiently degrade theelin (E1), 17beta-estradiol (E2) and 17alpha-ethinyl estradiol (EE2) and can be used for a micro-biological degradation technology for removing the estrogen. Under the culture condition of using the estrogen as the unique carbon source, the theelin and the 17beta-estradiol with the initial concentration of 2mg / L can be respectively degraded by 99 percent by the SS-2 strain within 36 hours, the 17alpha-ethinyl estradiol with the initial concentration of 4mg / L can be degraded by 93.6 percent within 168 hours, and estriol has no remarkable degradation phenomenon. The theelin is generated in the process of degrading the 17beta-estradiol, and the theelin is also gradually degraded. Because the pseudomonas citronellolis SS-2 strain has the characteristics of effectively degrading the natural and synthetic estrogen in an environment, the pseudomonas citronellolis SS-2 strain can be applied to an estrogen-contained waste sewage treatment technology or a soil remediation technology.

The invention relates to an oat acidovorax avenae strain capable of biodegrading natural estrogen and the application thereof, and the preservation code is CGMCC No. 3633; natural estrogen serves as the only one carbon source and is grown, can degrade estrone, 17Beta-estradiol and estriol with high efficiency, and can be used for a microbial technology for removing estrogen. The SS-1 strain can degrade the estrone with the initial concentration of 1mg / L by 99 percent within 12h, and degrade the 17Beta-estradiol and estriol with the initial concentration of 1mg / L by 99 percent within 24h. Estrone is generated in the degradation process of 17Beta-estradiol, and the estrone is also gradually degraded. Because the acidovorax avenae SS-1 strain has the property of effectively degrading the natural estrogen in the environment, and thereby can be applied in a technology for controlling wastewater and sewage which contain estrogen or a soil restoration technology.

The invention discloses compound bacteria capable of rapidly degrading 17-beta-estradiol as well as a preparation method and application of the compound bacteria. The compound bacteria contains rhodococcus rhodococcus DSH with a preservation number of CGMCC No.12392 and comamonas testosterone QYY with a preservation number of CGMCC No.15223, wherein the sequences of rhodococcus rhodococcus DSH and comamonas testosterone QYY are respectively presented by SEQ ID NO:1 and SEQ ID NO:2. The preparation method comprises the steps of respectively inoculating the strain DSH and the strain QYY into an inorganic salt culture medium, carrying out culturing so as to prepare DSH bacteria liquid and QYY bacteria liquid, and mixing the DSH bacteria liquid with the QYY bacteria liquid in a volume ratio of 1.5 to 1, so as to obtain the compound bacteria capable of rapidly degrading 17-beta-estradiol. The provided compound bacteria can rapidly degrade 17-beta-estradiol and has strong adaptive capacity to external environments. The compound bacteria is continuously cultured in the inorganic salt culture medium utilizing 17-beta-estradiol as a unique carbon source for 72 hours, so that 17-beta-estradiol with a concentration lower than 50mg / L can be rapidly and efficiently degraded, and the degradation rate reaches above 98%.

The method produces a lactose-free oral contraceptive composition containing a combination of a gestagen and an estrogen together with one or more pharmaceutically acceptable auxiliary agents and / or excipients. The contraceptive composition is a tablet, powder, or capsule that contains the gestagen and estrogen, filler material such as microcrystalline cellulose and a binder such as hydroxypropylcellulose, but no lactose. Preferably the gestagen is dienogest, chlormadinone acetate, or levonorgestrel and the estrogen is ethinylestradiol, 17β-estradiol, or estradiol valerate. A method is provided for improving the prophylaxis of lactose intolerance in women taking oral contraceptives. The oral contraceptive preparations for a standard 28-day cycle or for long-term use contain at least 21 daily dose units of the gestagen and the estrogen in a low-dosage but without lactose and at most 7 daily dose units containing no active ingredient or a placebo.

The invention relates to a water estrogen pollution detection method based on oryzias melastigma vitellogenin, and belongs to the field of environmental pollution detection. Currently, no marine fish is used for environment estrogen biological detection, and biological detection technology of seawater estrogen pollution is insufficient. According to the invention, euryhalic male oryzias melastigma with strong adaptability is selected as an in-vivo model; a primer is designed; the expression of vitellogenin (VTG1) is detected by a real-time quantitative PCR method; and 18S ribosome RNA (18S) is used as reference genes to establish a detection system of the VTG1 transcriptional level. Male fish is exposed to 17-beta-estradiol and clean artificial seawater, which are used as a positive and a negative control of the system, and appropriate exposure time is explored. The detection method can be used for detection of environment estrogen pollution degrees of seawater samples, river estuary samples, and saltwater internal lake water samples.

The invention relates to a preparation method of a photoelectrochemical 17 beta-estradiol aptamer sensor based on indium sulfide and cadmium sulfide co-sensitized cerium-doped titanium dioxide, and belongs to the field of photoelectrochemical sensors. According to the method, cerium-doped titanium dioxide is obtained by using a simple hydrothermal method to serve as a substrate material, and a light current is obtained; and for the cerium-doped titanium dioxide after indium sulfide sensitization, the photoelectric conversion efficiency is greatly improved, and the light absorption range is enlarged. Under the co-sensitization of cadmium sulfide, the positions of conduction bands of the three are reduced in a stepped mode, so that rapid transfer of electrons is facilitated, and an ideal light current signal is provided. The high-sensitivity detection of 17 beta-estradiol is realized by combining the specific recognition effect of an aptamer and the 17 beta-estradiol; the detection limitof the 17 beta-estradiol is 3.98 fg / mL; and the method has important significance in analysis and detection of the 17 beta-estradiol.

The present invention belongs to the field of auxiliary reproduction technology. Said invention described culture solution containing human mature follicular fluid is used for external culture technique of human immature ovum, and its composition is formed from Ham'e F-10 or TCM199, human serum substitute SSS, rFSH, HCG, 17 beta-estradiol and human mature follicular fluid. The in-vitro mature (IVM) technique of immature ovum can be used to take out the immature ovum of sterility woman with ovum maturation disturbance, and the culture solution prepared by said invention is used to culture it, and make it mature and make in-vitro fertilization and embryo transplantation to help gestation. It can obtain 65% of ovum mature rate and 33.3% of gestatino rate.

The invention provides a 17 beta estradiol-containing piperazine eutectic crystal as well as a preparation method and an application thereof. The X-ray powder diffraction analysis, thermogravimetic analysis, differential scanning calorimetry analysis and the like prove that the 17 beta estradiol-containing piperazine eutectic crystal provided by the invention has excellent physical chemistry property and medicine forming property. The preparation method of the eutectic crystal, provided by the invention, is simple to operate, easy to control and good in repeatability and can be used for obtaining the target eutectic crystal.