51results about How to "Suitable for standardization" patented technology

The invention discloses a high-yield s-linalool genetic engineering strain and a building method, which belong to the field of genetic engineering. Through the metabolic engineering reformation, linalool synthetase from actinidiaarguta is expressed in saccharomyces cerevisiae CEN.PK2-1 in a heterologous way, the terpene synthesis path-mevalonic acid (MVA) path carbon metabolizing flow is increased, and the high-yield s-linalool genetic engineering strain CEN. PK2 linalool is obtained. Compared with an initial strain CEN. PK2-1C, the modified genetic engineering strain CEN. PK2 linalool can metabolize and produce s-linalool, in addition, the feedback inhibition of the s-linalool is obviously reduced, the yield of the s-linalool can reach 12 mg / L, and the genetic engineering strain has good application prospects.

The invention discloses a method for improving gluconobacter oxydans for producing 2-keto-L-gulconic acid, and belongs to the technical field of genetic engineering. On the basis of genetic engineering transformation, adaptor protein SH3 and ligand protein SH3lig of the adaptor protein, a CutA gene and a key enzyme gene in saccharic acid transformation are subjected to fusion expression, and G.oxydans is transformed to produce 2-KLG in a one-step fermentation manner, so that the yield of 2-KLG is finally increased to be 40.3 g / L, that is, the yield is increased by 24.4% when being compared with that of a one-step engineering bacterium of which CutA is not expressed; in addition, due to the thermal resistance of foreign protein, the thermal resistance of the engineering bacterium is further improved. Enzyme cross-linking inside cells can be achieved by expressing the foreign protein CutA, continuous catalytic reaction of a plurality of enzymes can be facilitated, and the method has significance in is one-step fermentation production of vitamin C.

The invention discloses a method for measuring bisphenol A in a product by gas chromatography-mass spectrometry. The method comprises the following steps of: soaking and extracting bisphenol A in a sample with an organic solvent, and then carrying out silanization and derivatization; detecting the bisphenol A in the sample with a gas chromatography-mass spectrometer: setting the temperature of a sample injection port to be 250-300 DEG C, a flow rate to be 0.8-1.5ml / min and the temperature of a port to be 280-300 DEG C; enabling the sample to enter the sample injection port, carrying out temperature programming on the temperature of the chromatographic column from 40-60 DEG C to 250-320 DEG C; and enabling the sample to enter a mass spectrometric detection part through the port to carry out mass spectrometric detection, wherein an electron impact ion source is adopted as the ion source of the mass spectrometric detection, the impact energy of the ion source is 70eV, and the temperature of the ion source is set to be 230-250 DEG C. The method for measuring the bisphenol A in the product has low detection limit, good detection effect, little consumption of analysis solvent and low detection cost.

The invention provides automatic installation equipment and an installation method for a cantilever of a high-speed rail overhead line system. The automatic installation equipment comprises a cantilever assembly template, wherein the cantilever assembling template comprises a template platform, a flat cantilever mounting assembly and an inclined cantilever mounting assembly are arranged on the upper portion of the template platform, the flat cantilever mounting assembly and the inclined cantilever mounting assembly each comprise a clamping piece, and each clamping piece comprises an insulatorclamping piece body used for clamping an insulator and a cantilever clamping piece body used for clamping a cantilever; two cantilever fixing supports, wherein each cantilever fixing support is fixedon a supporting column and comprises a base which connected with the supporting column, and a double-lug clamping device corresponding to a single-lug connecting hole in the insulator is arranged on the base; and a mechanical arm used for carrying the cantilever assembling template to a stand column to be fixedly connected with the double-lug clamping devices of the cantilever fixing supports. Theautomatic installation equipment has the main advantages of labor saving, accurate installation, automatic alignment and high mechanization level.

The invention discloses a complete dropper prefabrication platform for a high speed railway overhead line system. The complete dropper prefabrication platform includes a rotatable fixing rod, a hydraulic clamp, a fixing block, a graduated scale, wire clippers, a sliding module and extrusion flat steel, the tip of the hydraulic clamp is welded and fixed at the top of the platform, the rotatable fixing rod is positioned on one side of the hydraulic clamp, the graduated scale is positioned on the other side of the hydraulic clamp, the sliding module is positioned on one side of the graduated scale and parallel to the graduated scale, the wire clippers are positioned on the side, far from the graduated scale, of the sliding module, and the wire clippers are fixed on the side, far from the rotatable fixing rod, of the hydraulic clamp. Through the reasonable layout of the hydraulic clamp, the wire clippers, the fixing block, the graduated scale, the sliding module and other functional modules on the platform, a complete dropper for the overhead line system is fabricated conveniently and accurately; since the whole prefabrication process is simple and easy to learn, an ordinary worker is capable of mastering the crimping essentials in 10 min and performing prefabrication independently, so that the efficiency and quality of the complete dropper prefabrication construction are enhanced. The complete dropper prefabrication platform is suitable for standardized and industrialized production.

The invention discloses a method for sealing a glass lamp tube and a metal electrode. The method includes the following steps that firstly, a metal ionic layer is implanted on the surface of the glass lamp tube; secondly, a layer of metal film is plated; thirdly, the glass lamp tube and the metal electrode are combined into a whole in a sealing mode through the high-temperature brazing technology with soldering flux. A lamp manufactured with the method has good mechanical strength and air tightness and is suitable for standard batch production.

The invention discloses an inorganic silicate heat insulating refractory and a preparation method thereof. The inorganic silicate heat insulating refractory comprises the following components in part by weight: 5-20 parts of attapulgite, 50-80 parts of meerschaum, 500-700 parts of water, 5-20 parts of ceramic fiber, 5-20 parts of bentonite, 5-20 parts of sodium sulfosuccinate, 5-20 parts of aluminum dihydrogen phosphate, 5-20 parts of sodium silicate, 1-10 parts of light magnesium carbonate, 1-10 parts of titanium oxide, 15-70 parts of expanded perlite, 5-20 parts of cement and 15-20 parts of sodium methyl silicate. The preparation method for the inorganic silicate heat insulating refractory comprises the following steps: stirring the water, the sodium sulfosuccinate and the ceramic fiber at the rotating speed of 20-30 rpm for 5-8 minutes; adding the meerschaum, stirring the mixture at the rotating speed of 50-70 rpm for 30-35 minutes and then adding the aluminum dihydrogen phosphate, the attapulgite, the bentonite, the sodium silicate, the light magnesium carbonate and the titanium oxide; and after 25 minutes, adding rest components and stirring for 10-15 minutes to obtain the required sizing.

The invention discloses a method for detecting a carcinogenic dye in a plastic packaging material, and the method combines high performance liquid chromatography and mass spectrometry. The method adopts the plastic packaging material as an object, sample pretreatment is simple, no special purification treatment is required, the concentration of the high content carcinogenic dye in a target productcan be accurately determined, and the method is suitable for standardization.

The invention belongs to the field of detection of saccharides and sugar alcohols in the food. An HPLC (high performance liquid chromatography) evaporative light scattering detection process for separating and detecting thirteen saccharides and sugar alcohols simultaneously comprises the following steps: gradiently eluting and separating a mixed standard solution of thirteen saccharides and sugar alcohols on an amino column through a mixed solution of acetonitrile and water as a separated flow phase with the eluting speed of 0.8 to 1.2ml / min; detecting the mixed standard solution after separation through an evaporative light scattering detector; and the detection conditions of the evaporative light scattering detector are as follows: the temperature of a drift tube is 40 to 70 DEG C, the carrier gas is N2, the flow velocity is 0.8 to 1.5l / min, and the gain is 1. The invention provides an analytic process for simultaneously separating and detecting multiple saccharides and sugar alcohols with high efficiency, convenience, low detection limit and high popularity.

The invention discloses a complete dropper prefabrication platform for a high speed railway overhead line system. The complete dropper prefabrication platform includes a rotatable fixing rod, a hydraulic clamp, a fixing block, a graduated scale, wire clippers, a sliding module and extrusion flat steel, the tip of the hydraulic clamp is welded and fixed at the top of the platform, the rotatable fixing rod is positioned on one side of the hydraulic clamp, the graduated scale is positioned on the other side of the hydraulic clamp, the sliding module is positioned on one side of the graduated scale and parallel to the graduated scale, the wire clippers are positioned on the side, far from the graduated scale, of the sliding module, and the wire clippers are fixed on the side, far from the rotatable fixing rod, of the hydraulic clamp. Through the reasonable layout of the hydraulic clamp, the wire clippers, the fixing block, the graduated scale, the sliding module and other functional modules on the platform, a complete dropper for the overhead line system is fabricated conveniently and accurately; since the whole prefabrication process is simple and easy to learn, an ordinary worker is capable of mastering the crimping essentials in 10 min and performing prefabrication independently, so that the efficiency and quality of the complete dropper prefabrication construction are enhanced. The complete dropper prefabrication platform is suitable for standardized and industrialized production.

The invention discloses a method for derivatization chromatographic analysis of residue semicarbazide in flour and a flour product. The method comprises the following steps: (1) enabling semicarbazide and a derivatization reagent to react, using HPLC / UV (High Performance Liquid Chromatography / Ultra Violet) to detect, and drawing to obtain a standard curve, wherein the standard curve takes the concentration of semicarbazideas an x-coordinate and takes the characteristic peak area of a derivatization product as a y-coordinate; (2) taking a to-be-detected sample, implementing ultrasonic extraction by using an extraction agent, centrifuging to obtain a supernate, adding the derivatization reagent and a precipitator into the supernate, centrifuging to obtain a supernate, filtering the supernate through an organic filter membrane to obtain a filtrate; (3) detecting the filtrate obtained in the step (2) through HPLC / UV, reading the characteristic peak area of the derivatization product, and contrasting with the standard curve to obtain the content of the semicarbazide in the to-be-detected sample. Compared with the prior art, the derivatization reagent strengthens the ultraviolet absorption of the derivatization product, and the detection is facilitated; in addition, a detection instrument is simple and common, the detection cost is low, the rapid detection of the semicarbazide is facilitated, and the method is applicable to large-scale sample detection and is suitable for standardization.

The invention relates to a curved-surface bow-shaped folded-plate tube shell type heat exchanger. The invention comprises a heater exchanger tube. The heater exchanger tube is connected with tube plate, and is provided inside with a the tube bundle passing through a group of tube holes of the bow-shaped folded plate and are fixed between the tube plates in the way of being parallel to axis; the folded plate is evenly fixed by the drawbar and the distance-fixed tube along the axis parallelly; the drawbar and the distance-fixed tube are fixed on the tube plate, the outer side of which is connected with tube box; the bow-shaped folded plate is the curved-surface bow-shaped folded plate, the outer convex side of which is directed at the inlet of the shell-process fluid; a group of bow-shaped folded plate gaps is arranged in a staggered way at intervals from top to bottom horizontally or from left to right vertically in order to form the circuitous flow channel of the fluid inside the tube. The heat exchanger of the invention has the advantages of improving the flow-speed distribution of the shell-process fluid of the bow-shaped folded plate, effectively increasing the circulation area, reducing 'dead zone', obviously improving the efficiency of the heat exchange and reducing the drop of the pressure in comparison to the flat-plated type bow-shaped folded plate.