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Method for establishing animal model with Lingnan damp-heat syndrome

A technology of animal model and establishment method, which is applied in the field of establishment of Lingnan damp-heat syndrome animal model, and achieves the effects of simple modeling method, good modeling stability and repeatability, and easy operation and control

Active Publication Date: 2020-03-13
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Based on this, it is necessary to provide a method for establishing an animal model of Lingnan damp-heat syndrome in view of the problems existing in the existing techniques for establishing animal damp-heat syndrome

Method used

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  • Method for establishing animal model with Lingnan damp-heat syndrome
  • Method for establishing animal model with Lingnan damp-heat syndrome
  • Method for establishing animal model with Lingnan damp-heat syndrome

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Experimental program
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Embodiment 1

[0039] 1 Model preparation materials and methods

[0040] 1.1 Animals: 12 SPF grade BALB / c mice, male and female, 3 weeks old, weighing 20-24g, provided by the Guangdong Provincial Experimental Animal Center, experimental animal license number: SYXK (Guangdong) 2015-0109, the small The mice were placed in the SPF animal room for adaptive feeding for one week, and the conditions were that the temperature was 22° C., the humidity was 55%, and the light time was 12 hours before the experiment was carried out.

[0041] 1.2 SPF litter and SPF feed

[0042] 1.3 Reagents:

[0043] Glycogen PAS staining solution (Beijing Legen Biotechnology Co., Ltd.)

[0044] Lendrum's fluorescent pink tartrazine (Paneth cell) staining kit (Shanghai Gefan Biotechnology Co., Ltd.)

[0045] Absolute ethanol (Sinopharm Chemical Reagent Co., Ltd.)

[0046] Xylene (Sinopharm Chemical Reagent Co., Ltd.)

[0047] EDTA(PH8.0)(Biyuntian)

[0048] PBS buffer (Gibco)

[0049] Antigen Retrieval Solution ...

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Abstract

The invention discloses a method for establishing an animal model with Lingnan damp-heat syndrome. The method comprises placing a mouse in a damp-heat environment for modeling feeding, and raising themouse for 14-28 days to obtain an animal model, wherein the mouse is fed with feed and packing during feeding, drinking water and the packing are changed every day, the damp-heat environment conditions include a daytime temperature of 34-36 DEG C with humidity of 85-90% for 12 h, and nighttime temperature of 18-22 DEG C with humidity of 55-65% for 12 h. The method for establishing the animal model with Lingnan characteristic damp-heat syndrome is more suitable for the climate, geographical environment and custom culture of the Lingnan area, a modeling method is simpler, modeling stability isgood, repeatability is good, and the operation and control are easy.

Description

technical field [0001] The invention relates to an animal model experiment technology, in particular to a method for establishing an animal model of Lingnan damp-heat syndrome. Background technique [0002] Damp-heat syndrome is a common TCM syndrome type, and its main manifestations are heaviness in the head and body, laziness, lack of speech, loose stools and other discomforts. In addition to mild discomfort, it can also be seen in many diseases, becoming a syndrome combined with other diseases. According to its etiology, it can be divided into exogenous damp-heat and endogenous damp-heat. Exogenous damp-heat is generally caused by environmental factors, and endogenous damp-heat is generally caused by eating emotions. It is generally believed that the two have interrelated mechanisms and pathways. . In the Lingnan area of ​​southeastern China, due to the hot and humid geographical environment, long hours of sunshine, high temperature, and abundant rain, it is easy to cau...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 骆欢欢陈颂郑裕华郑锋玲周祎青王婷谢婧
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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